A5101728370- Plant nutrient uptake and metabolism
- Plant Stress Responses and Tolerance
- Plant Molecular Biology Research
- Mycorrhizal Fungi and Plant Interactions
- Plant and Biological Electrophysiology Studies
- Legume Nitrogen Fixing Symbiosis
- Plant Reproductive Biology
- Advanced Data Storage Technologies
- Photosynthetic Processes and Mechanisms
- Plant Micronutrient Interactions and Effects
- Fungal Biology and Applications
- Soil and Land Suitability Analysis
- Advanced oxidation water treatment
- Peatlands and Wetlands Ecology
- Fungal and yeast genetics research
- Photoreceptor and optogenetics research
- Agriculture and Rural Development Research
- Electrochemical sensors and biosensors
- Plant responses to water stress
- Plant Genetic and Mutation Studies
- Pesticide and Herbicide Environmental Studies
- Ion channel regulation and function
- Plant and animal studies
- African Botany and Ecology Studies
- Forest Ecology and Biodiversity Studies
Université de Montpellier
2010-2022
Centre National de la Recherche Scientifique
2008-2022
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
2020-2022
Institut Agro Montpellier
2008-2022
Institut des Sciences des Plantes de Montpellier
2012-2021
L'Institut Agro
2021
The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in development. Its spatiotemporal distribution is controlled by combination of biosynthetic, metabolic and transport mechanisms. Four families transporters have been identified that mediate across the plasma or endoplasmic reticulum membrane. Here we report discovery functional characterization first vacuolar transporter. We demonstrate WALLS ARE THIN1 (WAT1), plant-specific protein dictates secondary cell wall thickness...
The phosphatase ABI2 enables plants to efficiently uptake and respond changes in nitrogen availability.
Abstract Ca2 +-dependent protein kinases (CPKs) form a large family of 34 genes in Arabidopsis (Arabidopsis thaliana). Based on their dependence Ca2+, CPKs can be sorted into three types: strictly Ca2+-dependent CPKs, Ca2+-stimulated (with significant basal activity the absence Ca2+), and essentially calcium-insensitive CPKs. Here, we report third type CPK, CPK13, which is expressed guard cells but whose role still unknown. We confirm expression CPK13 cells, show that its overexpression...
Calcium (Ca(2+)) is a second messenger involved in many plant signaling processes. Biotic and abiotic stimuli induce Ca(2+) signals within cells, which, when decoded, enable these cells to adapt response environmental stresses. Multiple examples of from plants containing the fluorescent yellow cameleon sensor (YC) have contributed definition signature some cell types such as root hairs, pollen tubes guard cells. YC is, however, limited use highly autofluorescent tissues, particular mesophyll...
Dipeptide (Leu-Leu) and nitrate transport activities of 26 Arabidopsis NPF (NRT1/PTR Family) proteins were screened in Saccharomyces cerevisiae Xenopus laevis oocytes, respectively. activity has been confirmed for 2 already known dipeptide transporters (AtNPF8.1 AtNPF8.3) but none the other tested NPFs displays transport. The screen resulted identification two new transporters, AtNPF5.5 AtNPF5.10. localization mRNA coding NPF5.5 demonstrates that it is first transporter reported to be...
Ectomycorrhizal symbiosis markedly improves plant phosphate uptake, but the molecular mechanisms underlying this benefit are still poorly understood. We identified two ESTs in a cDNA library prepared from ectomycorrhizal basidiomycete Hebeloma cylindrosporum with significant similarities to transporters endomycorrhizal fungus Glomus versiforme and non-mycorrhizal fungi. The full-length cDNAs corresponding these complemented yeast transport mutant (Deltapho84). Measurements of (33)P-phosphate...
Potassium (K+) is a key monovalent cation necessary for multiple aspects of cell growth and survival. In plants, this also plays role in the control stomatal movement. KAT1 its homolog KAT2 are main inward rectifying channels present guard cells, mediating K+ influx into these resulting opening. To gain further insight regulation channels, we performed split-ubiquitin protein-protein interaction screen searching interactors Arabidopsis (Arabidopsis thaliana). We characterized one candidates,...
Ectomycorrhizal symbiosis between fungi and woody plants strongly improves plant mineral nutrition constitutes a major biological process in natural ecosystems. Molecular identification functional characterization of fungal transport systems involved nutrient uptake are crucial steps toward understanding the improvement symbiotic relationship itself. In present report transporter belonging to Trk family is identified model ectomycorrhizal fungus Hebeloma cylindrosporum named HcTrk1. The...
Summary Iron (Fe) is an essential element, its transport regulated by the cell redox balance. In seeds, Fe enters embryo as 2+ and stored in vacuoles 3+ . Through ferric reduction activity, ascorbate plays a major role state therefore within seed. We searched for membrane transporters responsible controlling screening yeast reductase‐deficient fre1 strain isolated AtDTX25 , member of Multidrug And Toxic compound Extrusion (MATE) family. was shown to mediate efflux when expressed Xenopus...
Few proteins have been characterized as ABA transporters. Several of them are NPF transporters which in yeast using reporter systems. Because several members the NPF4 subfamily were identified transporters, here, we screened for transport activity seven Xenopus oocytes cRNA injection and 3H-ABA accumulation. The capacities NPF4.2; NPF4.5, NPF4.6 NPF4.7 confirmed. properties NPF4.5 studied more details. Both pH-dependent slightly apparent Km around 500 M. There is no competitive inhibition...
Constraints on plant growth imposed by low availability of nitrogen are a characteristic feature ecosystems dominated ectomycorrhizal plants. Ectomycorrhizal fungi play key role in the N nutrition plants, allowing their host plants to access decomposition products dead and animal materials. thus able compensate for inorganic forest ecosystems. The capacity take up peptides, as well transport mechanisms involved, were analysed fungus Hebeloma cylindrosporum. present study demonstrated that H....
Abstract Control of K+ and Na+ transport plays a central role in plant adaptation to salinity. In the halophyte Hordeum maritimum, we have characterized transporter gene, named HmHKT2;1, whose homolog HvHKT2;1 cultivated barley, vulgare, was known give rise increased salt tolerance when overexpressed. The encoded protein is strictly identical two H. maritimum ecotypes, from biotopes (Tunisian sebkhas) affected by different levels These ecotypes were found display distinctive responses stress...
Nitrate and potassium nutrition is tightly coordinated in vascular plants. Physiological molecular genetics studies have demonstrated that several NPF/NRT1 nitrate transporters a significant impact on both uptake the root-shoot partition of these nutrients. However, how traits are biochemically connected remain controversial since some NPF proteins, e.g. NPF7.3/NRT1.5, been suggested to mediate K+/H+ exchange instead fluxes. Here we show NPF6.2/NRT1.4, protein gates accumulation at leaf...
Abstract HKT1;5 loci/alleles are important determinants of crop salinity tolerance. HKT1;5s encode plasmalemma-localized Na+ transporters, which move xylem into parenchyma cells, reducing shoot accumulation. Allelic variation in rice OsHKT1;5 sequence specific landraces (Nona Bokra OsHKT1;5-NB/Nipponbare OsHKT1;5-Ni) correlates with salt Oryza coarctata, a halophytic wild rice, grows fluctuating at the seawater–estuarine interface Indian and Bangladeshi coastal regions. The distinct...
WAT1 (WALLS ARE THIN1) defines a novel auxin transporter in plants and integrates signaling secondary wall formation Arabidopsis fibers. IUFRO Tree Biotechnology Conference 2011: From Genomes to Integration Delivery
Post-translational regulations of Shaker-like voltage-gated K+ channels were reported to be essential for rapid responses environmental stresses in plants. In particular, it has been shown that calcium-dependent protein kinases (CPKs) regulate Shaker Here, the focus was on KAT2, a channel cloned model plant Arabidopsis thaliana, where is expressed namely vascular tissues leaves. After co-expression KAT2 with AtCPK6 Xenopuslaevis oocytes, voltage-clamp recordings demonstrated stimulates...
Abstract Potassium (K + ) plays crucial roles in many physiological, molecular and cellular processes plants. Direct uptake of this nutrient by root cells has been extensively investigated, however, indirect K mediated the interactions roots with fungi frame a mutualistic symbiosis, also called mycorrhizal pathway, is much less known. We identified an ion channel arbuscular (AM) fungus Rhizophagus irregularis . This exhibits canonical features Shaker-like shared other living kingdoms named...