A5101852359- Plant nutrient uptake and metabolism
- Legume Nitrogen Fixing Symbiosis
- Mycorrhizal Fungi and Plant Interactions
- Horticultural and Viticultural Research
- Plant Molecular Biology Research
- Plant responses to water stress
- Photosynthetic Processes and Mechanisms
- Plant Stress Responses and Tolerance
- Fermentation and Sensory Analysis
- Alzheimer's disease research and treatments
- Cholinesterase and Neurodegenerative Diseases
- Plant Reproductive Biology
- Nuts composition and effects
- Drug Transport and Resistance Mechanisms
- Biochemical and biochemical processes
- Plant biochemistry and biosynthesis
- Memory and Neural Mechanisms
- ATP Synthase and ATPases Research
- Legionella and Acanthamoeba research
- CRISPR and Genetic Engineering
- Metabolism, Diabetes, and Cancer
- Trace Elements in Health
- Lipid metabolism and biosynthesis
- Plant Micronutrient Interactions and Effects
- Infection Control and Ventilation
ETH Zurich
2004-2013
University of Zurich
2009-2013
Princeton University
2013
Pfizer (United States)
2010
Zero to Three
2005-2006
Timiryazev Institute of Plant Physiology
2004
Hungarian Academy of Sciences
2002-2003
Institute of Plant Biology
2002-2003
HUN-REN Szegedi Biológiai Kutatóközpont
2002-2003
The plant hormone auxin (indole-3-acetic acid, IAA) has a crucial role in development. Its spatiotemporal distribution is controlled by combination of biosynthetic, metabolic and transport mechanisms. Four families transporters have been identified that mediate across the plasma or endoplasmic reticulum membrane. Here we report discovery functional characterization first vacuolar transporter. We demonstrate WALLS ARE THIN1 (WAT1), plant-specific protein dictates secondary cell wall thickness...
Abstract Fructose (Fru) is a major storage form of sugars found in vacuoles, yet the molecular regulation vacuolar Fru transport poorly studied. Although SWEET17 (for SUGARS WILL EVENTUALLY BE EXPORTED TRANSPORTERS17) has been characterized as exporter leaves, its expression leaves low. Here, RNA analysis and SWEET17-β-glucuronidase/-GREEN FLUORESCENT PROTEIN fusions expressed Arabidopsis (Arabidopsis thaliana) reveal that highly cortex roots localizes to tonoplast root cells. Expression was...
Accumulation of anthocyanins in the exocarp red grapevine (Vitis vinifera) cultivars is one several events that characterize onset grape berry ripening (véraison). Despite our thorough understanding anthocyanin biosynthesis and regulation, little known about molecular aspects their transport. The participation ATP binding cassette (ABC) proteins vacuolar transport has long been a matter debate. Here, we present biochemical evidence an ABC protein, ABCC1, localizes to tonoplast involved...
Abstract Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the Sl SDH2-2 gene encoding iron sulfur subunit succinate dehydrogenase protein complex in antisense orientation under control 35S promoter exhibit an enhanced rate photosynthesis. The tricarboxylic acid (TCA) cycle was reduced these transformants, and there were changes levels metabolites associated with TCA cycle. Furthermore, comparison to wild-type plants, carbon dioxide assimilation by up 25% transgenic...
Solanaceous species are among the >200 000 plant worldwide forming a mycorrhiza, that is, root living in symbiosis with soil-borne arbuscular-mycorrhizal (AM) fungi. An important parameter of this symbiosis, which is vital for ecosystem productivity, agriculture, and horticulture, transfer phosphate (Pi) from AM fungus to plant, facilitated by plasma membrane-spanning Pi transporter proteins. The first mycorrhiza-specific be identified, was StPT3 potato [Nature414 (2004) 462]. Here, we...
Abstract: Maize is one of the most important crops in developing world, where adverse soil conditions and low fertilizer input are two main constraints for stable food supply. Understanding molecular biochemical mechanisms involved nutrient uptake expected to support development future breeding strategies aimed at improving maize productivity on infertile soils. Phosphorus least mobile macronutrient soils it often limiting plant growth. In this work, five genes encoding Pht1 phosphate...
Arabidopsis possesses a superfamily of ATP-binding cassette (ABC) transporters. Among these, the multidrug resistance-associated protein AtMRP5/AtABCC5 regulates stomatal aperture and controls plasma membrane anion channels guard cells. Remarkably, despite prominent role AtMRP5 in conferring partial drought insensitivity upon Arabidopsis, we know little biochemical function AtMRP5. Our phylogenetic analysis showed that is closely related to maize MRP4, mutation which confers low inositol...
• Plants colonized by arbuscular mycorrhizal (AM) fungi take up phosphate (Pi) via the and direct Pi uptake pathway. Our understanding of molecular mechanisms involved in regulation these pathways is just emerging. Here, we have analyzed physiology tomato (Solanum lycopersicum) variety Micro-Tom integrated data obtained with studies on chemical signaling mycorrhiza-inducible transporter gene regulation. At high plant phosphorus (P) status, pathway was almost completely repressed genes were...
Arbuscular mycorrhizae are ancient symbioses that thought to have originated >400 million years ago in the roots of plants, pioneering colonization terrestrial habitats. In these associations, a key process is transfer phosphorus as inorganic phosphate host plant across fungus–plant interface. Mycorrhiza-specific transporter genes and their regulation conserved phylogenetically distant species, they activated selectively by fungal species from phylum Glomeromycota . The potato gene StPT3...
Abscisic acid (ABA) is a key plant hormone involved in diverse physiological and developmental processes, including abiotic stress responses the regulation of stomatal aperture seed germination. glucosyl ester (ABA-GE) hydrolyzable ABA conjugate that accumulates vacuole presumably also endoplasmic reticulum. Deconjugation ABA-GE by reticulum vacuolar β-glucosidases allows rapid formation free response to conditions such as dehydration salt stress. further contributes maintenance homeostasis,...
Summary Induction of knockout mutations by T‐DNA insertion mutagenesis is widely used in studies plant gene functions. To assess the efficiency this genetic approach, we have sequenced PCR amplified junctions 1000 insertions and analysed their distribution Arabidopsis genome. Map positions 973 tags could be determined unequivocally, indicating that majority landed chromosomal domains high density. Only 4.7% were found interspersed, centromeric, telomeric rDNA repeats, whereas 0.6% identified...
Arbuscular mycorrhizal fungi (AMF) form a mutually beneficial symbiosis with plant roots providing predominantly phosphorus in the of orthophosphate (Pi) exchange for carbohydrates on low P soils. The goal this work was to generate molecular-genetic evidence support major impact Pi uptake (MPU) pathway productivity crop maize under field and controlled conditions. Here we show, that loss-of-function mutation mycorrhiza-specific transporter gene Pht1;6 correlates dramatic reduction...
BACE1 is the rate-limiting enzyme that cleaves amyloid precursor protein (APP) to produce β peptides accumulate in Alzheimer's disease (AD). BACE1, which elevated AD patients and APP transgenic mice, also β2-subunit of voltage-gated sodium channels (Navβ2). Although increased levels are associated with Navβ2 cleavage patients, whether occurs mice had not yet been examined. Such a finding would be interest because its potential impact on neuronal activity: previous studies demonstrated...
Summary A Lycopersicon esculentum mutant ( rmc ) is resistant to colonization by most arbuscular mycorrhizal fungi (AMF), but one Glomus intraradices isolate (WFVAM 23) develops arbuscules and vesicles in the cortex. It unknown whether symbiotic phosphate (Pi)‐uptake pathway operational this interaction. Hyphal uptake of 32 Pi expression plant transporter genes were investigated its wild‐type progenitor (76R) associated with three AMF. Hyphae transferred all symbioses 76R – G. WFVAM 23...
The plant vacuole is the largest compartment in a fully expanded cell. While only very limited metabolic activity can be observed within vacuole, majority of hydrolytic activities, including proteolytic activities reside this organelle. Since it assumed that protein degradation by proteasome results production peptides with size 3-30 amino acids, we were interested to show whether tonoplast exhibits transport activity, which could deliver these into for final degradation. It shown here...
Recent studies have demonstrated that soluble Aβ dimers isolated from AD brains inhibit long-term potentiation (LTP), reduce dendritic spine density in vitro and upon direct injection into the brain of normal rats disrupt memory (Shankar et al., 2008). In current study we evaluated acquisition performance PSAPP mice a Morris water maze, searched for oligomers transgenic to determine whether has similar biochemical properties human extracted brains. Wild type (wt) heterozygous were trained...