A5004262266- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant Stress Responses and Tolerance
- Photosynthetic Processes and Mechanisms
- Plant nutrient uptake and metabolism
- Light effects on plants
- Plant tissue culture and regeneration
- Plant Parasitism and Resistance
- Plant responses to water stress
- Chromosomal and Genetic Variations
- Soybean genetics and cultivation
- Plant Virus Research Studies
- Plant Genetic and Mutation Studies
- Genomics and Chromatin Dynamics
- GABA and Rice Research
- CRISPR and Genetic Engineering
- Plant responses to elevated CO2
- Plant Taxonomy and Phylogenetics
- Plant Growth and Agriculture Techniques
- Neurobiology and Insect Physiology Research
- melanin and skin pigmentation
- Cellular transport and secretion
- interferon and immune responses
- Plant-Microbe Interactions and Immunity
Nara Institute of Science and Technology
2015-2025
The University of Tokyo
2007-2024
Chubu University
2024
Tokyo Institute of Technology
2024
Japan Science and Technology Agency
2018-2021
Universitas Gadjah Mada
2021
University of Pennsylvania
2011-2020
Bio-Medical Science (South Korea)
2016
South University
2014
Tokyo University of Science
2011-2013
Coordination of cell division and pattern formation is central to tissue organ development, particularly in plants where walls prevent migration. Auxin cytokinin are both critical for patterning, but it unknown how these hormones converge upon development. We identify a genetic network that reinforces an early embryonic bias auxin distribution create local, nonresponding source within the root vascular tissue. Experimental theoretical evidence shows cells act as organizer by positioning...
One Hormone, Two Phases The switch from vegetative growth to flowering in the plant Arabidopsis involves two phases—inflorescence branching and flowering. Yamaguchi et al. (p. 638 ) examined how phytohormone gibberellin regulates each phase differently. First, levels increase stimulate production of key factors, one which degrades gibberellin. As then fall, next factors is released repression. By regulating inflorescence separately flowering, this system determines overall seed yield.
Reprogramming of cell identities during development frequently requires changes in the chromatin state that need to be restricted correct populations. Here we identify an auxin hormone-regulated switch directs reprogramming from transit amplifying primordium founder fate Arabidopsis inflorescences. Upon sensing, MONOPTEROS transcription factor recruits SWI/SNF remodeling ATPases increase accessibility DNA for induction key regulators flower initiation. In absence hormonal cue, sensitive...
Abstract Plants monitor seasonal cues to optimize reproductive success by tuning onset of reproduction and inflorescence architecture. TERMINAL FLOWER 1 (TFL1) FLOWERING LOCUS T (FT) their orthologs antagonistically regulate these life history traits, yet mechanism action, antagonism targets remain poorly understood. Here, we show that TFL1 is recruited thousands loci the bZIP transcription factor FD. We identify master regulator floral fate, LEAFY ( LFY ) as a target under dual opposite...
Acclimation to high temperature increases plants' tolerance of subsequent lethal temperatures. Although epigenetic regulation plant gene expression is well studied, how plants maintain a memory environmental changes over time remains unclear. Here, we show that JUMONJI (JMJ) proteins, demethylases involved in histone H3 lysine 27 trimethylation (H3K27me3), are necessary for Arabidopsis thaliana heat acclimation. induces sustained H3K27me3 demethylation at HEAT SHOCK PROTEIN22 (HSP22) and...
Patterning of the floral organs is exquisitely controlled and executed by four classes homeotic regulators. Among these, class B C regulators are central importance as they specify male female reproductive organs. Inappropriate induction gene APETALA3 ( AP3 ) AGAMOUS AG causes reduced fitness prevented polycomb repression. At onset flower patterning, repression needs to be overcome allow formation We show that SWI2/SNF2 chromatin-remodeling ATPases SPLAYED (SYD) BRAHMA (BRM) redundantly...
The ability of proteins to associate with genomic DNA in the context chromatin is critical for many nuclear processes including transcription, replication, recombination, and repair. Chromatin immunoprecipication (ChIP) a practical useful technique characterizing protein / association vivo. procedure generally includes six steps: (1) crosslinking DNA; (2) isolating chromatin; (3) fragmentation; (4) imunoprecipitation antibodies against interest; (5) recovery; (6) PCR identification factor...
To ensure successful plant reproduction and crop production, the spatial temporal control of termination floral meristem must be coordinated. In Arabidopsis, timing this is determined by AGAMOUS (AG). Following its termination, underdoes gynoecium formation. A direct target AG, CRABS CLAW (CRC), involved in both determinacy development. However, how coordinated with formation not understood. Here, we identify a mechanistic link between development through fine-tuning auxin homeostasis CRC....
Arabidopsis (
In angiosperms, the transition from floral-organ maintenance to abscission determines reproductive success and seed dispersion. For petal abscission, cell-fate decisions specifically at petal-cell base are more important than organ-level senescence or cell death in petals. However, how this is regulated remains unclear. Here, we identify a jasmonic acid (JA)-regulated chromatin-state switch of Arabidopsis petals that directs local determination via autophagy. During maintenance,...
Abstract Co-option of gene regulatory networks leads to the acquisition new cell types and tissues. Stomata, valves formed by guard cells (GCs), are present in most land plants regulate CO 2 exchange. The transcription factor (TF) FAMA globally regulates GC differentiation. In Brassicales, also promotes development idioblast myrosin (MCs), another type specialized along vasculature essential for Brassicales-specific chemical defences. Here we show that Arabidopsis thaliana , directly induces...
Summary SWI2/SNF2 chromatin remodeling ATPases play important roles in plant and metazoan development. Whereas metazoans generally encode one or two ATPase genes, Arabidopsis encodes four such regulators: the well‐studied BRAHMA SPLAYED ATPases, as well closely related non‐canonical CHR12 CHR23. No developmental role has yet been described for Here, we show that although strong single chr12 chr23 mutants are morphologically indistinguishable from wild type, double cause embryonic lethality....
The switch from producing vegetative structures (branches and leaves) to reproductive (flowers) is a crucial developmental transition that significantly affects the success of flowering plants. In Arabidopsis, this in large part controlled by meristem identity regulator LEAFY (LFY). molecular mechanisms which LFY orchestrates precise robust flower formation not well understood. Here, we show direct target LATE MERISTEM IDENTITY2 (LMI2) has role transition. Like LFY, LMI2 activates AP1...
Abstract In flowering plants, the switch from floral stem cell maintenance to gynoecium (female structure) formation is a critical developmental transition for reproductive success. Arabidopsis thaliana , AGAMOUS (AG) terminates activities trigger this transition. Although CRABS CLAW ( CRC ) direct target of AG, previous research has not identified any common targets. Here, we identify an auxin synthesis gene, YUCCA4 YUC4 as target. Ectopic expression partially rescues indeterminate...
Proper timing of the onset to flower formation is critical for reproductive success. Monocarpic plants like Arabidopsis (Arabidopsis thaliana) switch from production branches in axils leaves that flowers once their lifecycle, during meristem identity transition. The plant-specific transcription factor LEAFY (LFY) necessary and sufficient this Previously, we reported plant hormone auxin induces LFY expression through AUXIN RESPONSE FACTOR5/MONOPTEROS (ARF5/MP). It not known whether MP solely...
Summary Lateral root (LR) formation in Arabidopsis thaliana is initiated by asymmetric division of founder cells, followed coordinated cell proliferation and differentiation for patterning new primordia. The sequential developmental processes LR are triggered a localized auxin response. LATERAL ORGAN BOUNDARIES‐DOMAIN 16 (LBD16), an auxin‐inducible transcription factor, one the key regulators linking response cells to initiation. We identified genes that activated LBD16 auxin‐dependent...
After germination, seedlings undergo growth arrest in response to unfavourable conditions, a critical adaptation enabling plants survive harsh environments. The plant hormone abscisic acid (ABA) plays key role this arrest. To growth, ABA-dependent transcription factors change gene expression patterns flexible and reversible manner. Although the control of has important roles arrest, epigenetic mechanisms ABA are not fully understood. Here, we show that histone demethylases JUMONJI-C...