The seeds of Zea mays L. cv. KWS were exposed to low-temperature plasma (LTP) by using Diffuse Coplanar Surface Barrier Discharge (DCSBD) for 60 and 120 seconds respectively. Growth parameters, anatomy roots activity some enzymes (CAT, G-POX, SOD DHO) isolated from grown the treated LTP evaluated. Our results indicate that treatment maize affects post-germination growth seedlings this effect depends on duration treatment. in significantly increased length, fresh dry weight roots. However,...

Background and Aims Deposition of silica in plant cell walls improves their mechanical properties helps plants to withstand various stress conditions. Its mechanism is still not understood silica–cell wall interactions are elusive. The objective this study was investigate the effect deposition on development structure sorghum root endodermis identify components involved silicification. MethodsSorghum bicolor seedlings were grown hydroponically with (Si+) or without (Si−) silicon...

Background and Aims In the present study, we show that development of endodermis exodermis is sensitively regulated by water accessibility. As cadmium (Cd) known to induce xeromorphic effects in plants, maize roots were exposed also Cd understand developmental process suberin lamella deposition response a local source. Methods first experiment, cultivated vitro unilaterally water-containing medium from one side air other. second placed between two agar layers with strip Cd-containing...

Merwilla plumbea is an important African medicinal plant. As the plants grow in soils contaminated with metals from mining activities, danger of human intoxication exists. An experiment exposed to cadmium (Cd) was performed investigate response M. this heavy metal, its uptake and translocation plant organs reaction root tissues. Plants grown seeds were cultivated controlled conditions. Hydroponic cultivation not suitable for species as roots do tolerate aquatic conditions, additional stress...

Acanthamoebae success as human pathogens is largely due to the highly resistant cysts which represent a crucial problem in treatment of Acanthamoeba infections. Hence, study cyst wall composition and encystment play an important role finding new therapeutic strategies. For first time, we detected high activity cytoskeletal elements - microtubular networks filamentous actin, late phases encystment. Cellulose fibrils main components endocyst were demonstrated inter-cystic space, finally...

Summary Background The SWI/SNF chromatin remodelling complex plays important roles in cellular processes including cell differentiation, cycle control and DNA repair. Aberrant expression of subunits is involved cancer development. core subunit the complex, SNF5, has been shown to be inactivated malignant rhabdoid tumours defined as a tumour suppressor. However, role catalytic subunit, BRG1, not well cancer. Objectives To investigate BRG1 melanoma development, we examined melanocytic lesions...

Background Autoimmune attack of the bulbar region anagen phase hair follicles by CD8+ T cells and Th1 cytokines has been proposed to result in loss alopecia areata (AA). The initiating stimuli are unknown. As interferon-α therapy may trigger AA, we propose that type 1 interferons involved induction disease. Objectives To compare lesional scalp from patients with AA lesions cutaneous diseases associated local interferon-related protein expression. Methods Lesional discoid lupus erythematosus,...

Cutaneous melanoma is a life-threatening skin cancer because of its poorly understood invasive nature and high metastatic potential. This study examines the importance eukaryotic translation initiation factor 5A2 (EIF5A2) in pathogenesis. We examined EIF5A2 expression 459 melanocytic lesions using tissue microarray. In addition, cell lines were subjected to invasion proliferation assays, zymography, FACS real-time PCR investigate role progression. Positive staining increased from dysplastic...

Cutting plant material is essential for observing internal structures and may be difficult various reasons. Most fixation agents such as aldehydes, well embedding resins, do not allow subsequent use of fluorescent staining make too soft to good-quality hand-sections. Moreover, cutting thin roots can very time consuming. A new, fast effective method provide sections fresh or fixed root samples, including those (such Arabidopsis Noccaea), described here. To overcome the above-mentioned...

Malignant melanoma is a life-threatening skin cancer due to its highly metastatic character and resistance radio- chemotherapy. It believed that the ability evade apoptosis key mechanism for rapid growth of cells. However, exact failure in apoptotic pathway cells unclear. p53, most frequently mutated tumour suppressor gene human cancers, inducer. p53 mutation only found 15–20% biopsies. Recently, it was Apaf-1, downstream target inactivated melanoma. Specifically, loss heterozygosity (LOH)...

Cutaneous malignant melanoma is a severe and sometimes life-threatening cancer. The molecular mechanism of melanomagenesis incompletely understood. Deregulation apoptosis probably one the key factors contributing to progression melanoma. inhibitor growth (ING) family proteins are candidate tumour suppressors which play important roles in apoptosis. Downregulated expression ING have been reported several types, including loss nuclear p33ING1b As ING2 exhibits 58.9% homology with p33ING1b, we...

Apoplasmic barriers in plants fulfil important roles such as the control of apoplasmic movement substances and protection against invasion pathogens. The aim this study was to describe development (Casparian bands suberin lamellae) endodermal cells Arabidopsis thaliana primary root during lateral initiation. Modifications cell walls roots wild-type Landsberg erecta (Ler) mutants with defective – scarecrow-3 (scr-3) shortroot (shr) A. were characterized by light, fluorescent, confocal laser...

Mutation of BRAF is a prevalent event in melanoma. Despite much attention to the role mutation melanoma, status protein expression and its significance melanoma progression are unknown.We investigated level different stages melanocytic lesions evaluated correlation with clinicopathological features patient survival.Using tissue microarray, outcome was 49 naevi samples 370 patients We also V600E using direct sequencing.Compared samples, remarkably increased primary melanomas further...

Root hairs increase the contact area of roots with soil and thereby enhance capacity for solute uptake. The strict hair/non-hair pattern Arabidopsis thaliana can change nutrient deficiency or exposure to toxic elements, which modify root hair density. effects density on cadmium (Cd) accumulation in shoots arabidopsis genotypes altered development patterning were studied.Arabidopsis mutants that are unable develop (rhd6-1 cpc/try) produce hairy (wer/myb23) compared ecotype Columbia (Col-0)....

The E3 ligase Rad18 is a key regulator for the lesion bypass pathway, which plays an important role in genomic stability. However, status of expression melanoma not known. Using tissue microarray (TMA), we showed that nuclear was upregulated primary and metastatic compared to dysplastic nevi. significantly reduced sun-exposed sites sun-protected sites. Strong correlated with worse 5-year patient survival independent prognostic factor found Furthermore, cell proliferation pAkt cyclin D1 were...