A5073523453- Seed Germination and Physiology
- Plant Parasitism and Resistance
- Soybean genetics and cultivation
- Allelopathy and phytotoxic interactions
- Nitrogen and Sulfur Effects on Brassica
- Plant Genetic and Mutation Studies
- Microbial Metabolic Engineering and Bioproduction
- Plant Reproductive Biology
- Genetic and Environmental Crop Studies
- Weed Control and Herbicide Applications
- Geology and Paleoclimatology Research
- Plant Molecular Biology Research
- Photosynthetic Processes and Mechanisms
- Lipid metabolism and biosynthesis
- Botanical Research and Chemistry
- Plant tissue culture and regeneration
- Sunflower and Safflower Cultivation
- Plant Ecology and Taxonomy Studies
- Electron Spin Resonance Studies
- Cryospheric studies and observations
- Plant Pathogens and Fungal Diseases
- Tree Root and Stability Studies
- GABA and Rice Research
- Soil Moisture and Remote Sensing
- Plant Water Relations and Carbon Dynamics
Royal Holloway University of London
2012-2024
University of Freiburg
2009-2014
Leibniz University Hannover
2013-2014
Significance Mechanisms of plant seed dormancy evolved to delay germination a season favorable for seedling growth. Germination timing is an important adaptive early-life history trait which determines fitness in natural and agricultural ecosystems. The DELAY OF GERMINATION 1 ( DOG1 ) gene provides genetic variation dormancy, was the first dormancy-specific cloned, encodes protein unknown function. We show here that controls different species by setting optimal ambient temperature window...
Abstract Comparative biology includes the comparison of transcriptome and quantitative real-time RT-PCR (qRT-PCR) data sets in a range species to detect evolutionarily conserved divergent processes. Transcript abundance analysis target genes by qRT-PCR requires highly accurate robust workflow. This reference with high expression stability (i.e., low intersample transcript variation) for correct gene normalization. Cross-species proper comparative quantification suitable different species. We...
Understanding of seed ageing, which leads to viability loss during storage, is vital for ex situ plant conservation and agriculture alike. Yet the potential regulation at transcriptional level has not been fully investigated. Here, we studied relationship between viability, gene expression glutathione redox status artificial ageing pea (Pisum sativum) seeds. Transcriptome-wide analysis using microarrays was complemented with qRT-PCR selected genes a multilevel antioxidant glutathione....
Understanding how plants cope with changing habitats is a timely and important topic in plant research. Phenotypic plasticity describes the capability of genotype to produce different phenotypes when exposed environmental conditions. In contrast, constant production set distinct by one mediates bet hedging, strategy that reduces temporal variance fitness at expense lowered arithmetic mean fitness. Both phenomena are thought represent adaptation strategies unstable environments. However,...
The timing of seed germination is crucial for plants and coordinated by internal external cues, reflecting adaptations to different habitats. Physiological molecular studies with lettuce Arabidopsis thaliana have documented a strict requirement light initiate identified many receptors, signaling cascades, hormonal control elements. In contrast, in several other inhibited light, but the basis this alternative response unknown. We describe Aethionema arabicum (Brassicaceae) as suitable model...
Plants in habitats with unpredictable conditions often have diversified bet-hedging strategies that ensure fitness over a wider range of variable environmental factors. A striking example is the diaspore (seed and fruit) heteromorphism evolved to maximize species survival Aethionema arabicum (Brassicaceae) which external endogenous triggers allow production two distinct diaspores on same plant. Using this dimorphic model, we identified contrasting molecular, biophysical, ecophysiological...
Myrigalone A (MyA) is a rare flavonoid in fruit leachates of Myrica gale, deciduous shrub adapted to flood-prone habitats. As putative allelochemical it inhibits seed germination and seedling growth. Using Lepidium sativum as model target species, experiments were conducted investigate how environmental cues modulate MyA's interference with key processes germination. Time course analyses L. testa endosperm rupture under different light conditions water potentials combined quantifying...
Abstract The biomechanical and ecophysiological properties of plant seed/fruit structures are fundamental to survival in distinct environments. Dispersal fruits with hard pericarps (fruit coats) encasing seeds has evolved many times independently within taxa that have seed dispersal as their default strategy. mechanisms by which the constraint a pericarp determines germination timing response environment currently unknown. Here, we show Lepidium didymum controls solely mechanism. Mechanical...
RNA-sequencing analysis is increasingly utilized to study gene expression in non-model organisms without sequenced genomes. Aethionema arabicum (Brassicaceae) exhibits seed dimorphism as a bet-hedging strategy - producing both less dormant mucilaginous (M+) morph and more non-mucilaginous (NM) morph. Here, we compared de novo reference-genome based transcriptome assemblies investigate Ae. evaluate the reference-free versus -dependent approach for identifying differentially expressed genes...
Abstract Seed dormancy is a block to the completion of germination an intact viable seed under favorable conditions and adaptive agronomically important trait. Thus, elucidating conserved features mechanisms great interest. The worldwide-distributed genus Lepidium (Brassicaceae) well suited for cross-species comparisons investigating origin common or specific early-life-history traits. We show here that homologs dormancy-specific gene DELAY OF GERMINATION1 (DOG1) from Arabidopsis...
Understanding of seed ageing, which leads to viability loss during storage, is vital for ex situ plant conservation and agriculture alike.Yet the potential regulation at transcriptional level has not been fully investigated.Here, we studied relationship between viability, gene expression glutathione redox status artificial ageing pea (Pisum sativum) seeds.Transcriptome-wide analysis using microarrays was complemented with qRT-PCR selected genes a multilevel antioxidant glutathione.Partial...
Secondary dormancy is an adaptive trait that increases reproductive success by aligning seed germination with permissive conditions for seedling establishment. Aethionema arabicum annual plant and member of the Brassicaceae grows in environments characterized hot dry summers. seeds may germinate early spring when establishment permissible. We demonstrate long-day light regimes induce secondary (CYP accession) repressing summer riskier. Characterization mutants screened defective demonstrated...
Abstract Plants in habitats with unpredictable conditions are often characterized by diversifying their bet-hedging strategies that ensure fitness over a wider range of variable environmental factors. A striking example is the diaspore (seed and fruit) heteromorphism evolved to maximize species survival Aethionema arabicum (Brassicaceae) which external endogenous triggers allow production two distinct diaspores on same plant. Using this dimorphic model, we identified contrasting molecular,...
Abstract Timing of seed germination is crucial for plants and coordinated by internal external cues, reflecting adaptations to different habitats. Physiological molecular studies with lettuce Arabidopsis thaliana have documented a strict requirement light initiate identified many receptors, signalling cascades, hormonal control elements. In contrast, several other inhibited light, but the basis this converse response unknown. We describe Aethionema arabicum (Brassicaceae) as suitable model...