A5060711247- Plant Genetic and Mutation Studies
- Plant tissue culture and regeneration
- Chromosomal and Genetic Variations
- Plant Molecular Biology Research
- Plant Stress Responses and Tolerance
- DNA Repair Mechanisms
- Seed Germination and Physiology
- Carcinogens and Genotoxicity Assessment
- CRISPR and Genetic Engineering
- Cancer therapeutics and mechanisms
- Plant Virus Research Studies
- Plant Physiology and Cultivation Studies
- Aluminum toxicity and tolerance in plants and animals
- Plant Micronutrient Interactions and Effects
- Plant and Fungal Interactions Research
- Soybean genetics and cultivation
- Genomics and Phylogenetic Studies
- Plant Reproductive Biology
- Plant Disease Resistance and Genetics
- Genomics and Chromatin Dynamics
- Garlic and Onion Studies
- Legume Nitrogen Fixing Symbiosis
- Plant and animal studies
- Transgenic Plants and Applications
- Sunflower and Safflower Cultivation
Austrian Academy of Sciences
2015-2024
Gregor Mendel Institute of Molecular Plant Biology
2014-2024
Vienna Biocenter
2015-2024
University of Pavia
2009-2016
Institute of Genetics and Biophysics
2012-2013
Biogem
2010
In plants, aerial organs originate continuously from stem cells in the center of shoot apical meristem. Descendants subepidermal layer are progenitors germ cells, giving rise to male and female gametes. these mutations, including insertions transposable elements or viruses, must be avoided preserve genome integrity across generations. To investigate molecular characteristics Arabidopsis, we isolated their nuclei analyzed stage-specific gene expression DNA methylation plants different ages....
The germination test currently represents the most used method to assess seed viability in germplasm banks, despite difficulties caused by occurrence of dormancy. Furthermore, longevity can vary considerably across species and populations from different environments, studies related eco-physiological processes underlying such variations are still limited their depth. aim present work was identification reliable molecular markers that might help monitoring deterioration. Dry seeds were...
Seed longevity, a fundamental plant trait for ex situ conservation and persistence in the soil of many species, varies across populations generations that experience different climates. This study investigates extent to which differences seed longevity are due genetic and/or modified by adaptive responses environmental changes. Seeds two wild Silene vulgaris from alpine (wA) lowland (wL) locations seeds originating their cultivation common garden (cA1, cL1, cA2 cL2) were exposed controlled...
SUMMARY Individual cells give rise to diverse cell lineages during the development of multicellular organisms. Understanding contribution these mature organisms is a central question developmental biology. Several techniques document have been used, from marking single with mutations that express visible marker generating molecular bar codes by CRISPR‐induced and subsequent single‐cell analysis. Here, we exploit mutagenic activity CRISPR allow lineage tracing within living plants reporter....
Abstract In sexually propagating organisms, genetic, and epigenetic mutations are evolutionarily relevant only if they occur in the germline hence transmitted to next generation. contrast most animals, plants considered lack an early segregating germline, implying that somatic cells can contribute genetic information progeny. Here we demonstrate 2 ARGONAUTE proteins, AGO5 AGO9, mark associated with sexual reproduction Arabidopsis (Arabidopsis thaliana) throughout development. Both AGOs...
An intron-spliced hairpin RNA approach was used for the targeted silencing of MtTdp1α gene encoding αisoform tyrosyl-DNA phosphodiesterase 1 in Medicago truncatula Gaertn. Tyrosyl-DNA 1, involved repair DNA topoisomerase I-mediated damage, has been poorly investigated plants. RNA-Seq analysis, carried out MtTdp1α-depleted plants, revealed different levels transcriptional modulation (up- and down-regulation, alternative splicing, activation promoter) genes damage sensing, repair, chromatin...
In animal cells, recent studies have emphasized the role played by DNA topoisomerase I (topo I) both as a cofactor of repair complexes and/or damage sensor. All these functions are still unexplored in plant where information concerning relationships between damage, PCD induction, and topo also limited. The main goal this study was to investigate possible responses activated I-depleted cells under oxidative stress conditions which induce damage. carrot (Daucus carota L.) AT1-β/22 cell line...
Abstract The discovery of CRISPR/Cas9 has revolutionized molecular biology, and its impact on plant biotechnology breeding cannot be over‐estimated. In many species, application for mutagenesis is now a routine procedure––if suitable target sites, sufficient expression the Cas9 protein, functioning sgRNAs are combined. differ in their efficiency, depending parameters that only poorly understood. Several software tools experience from growing databases supporting design sgRNAs, but some...
Abstract Efficient repair of DNA lesions is essential for the faithful transmission genetic information between somatic cells and genome integrity across generations. Plants have multiple, partially redundant, overlapping pathways, probably due to less constricted germline inevitable exposure light including higher energy wavelengths. Many proteins involved in their mode actions are well described. In contrast, a role damage-associated RNA components, evident from many other organisms,...
Roses (Rosa hybrida) are the most important ornamental cut-flowers and breeders’ main focus is to develop new desirable modern cultivars. Rose breeding programs center on introduction of flower colors, thornless stems, higher production good post-harvest performance. The study pollen traits, such as quantity quality, viability, longevity, morphological homogeneity, germination tube growth, for building suitable programs. Recently, a number studies have shown that reactive oxygen species,...
Genome stability, an essential requisite for successful seed germination/seedling establishment, is maintained through the cellular DNA repair machinery which works in concert with chromatin remodeling regulators/effectors. To date, correlation between and vigour has been established while information still missing as concerns contribution of to quality enhancement. The role played by telomere maintenance mechanisms relation debated. In present work, a bioinformatic investigation was...
Modern rose cultivars require hand pollination for hip production and collection of seeds.Breeding programs are often focused on the quality pollen, which is genotype dependent it affected by conditions used its conservation.In this study in vitro pollen germination shelf life six commercial were evaluated under different storage order to establish preservation procedures.Flowers Rosa 'Alba', 'Anastasia', 'Encanto', 'Marvelle', 'Swan' 'Touch Class' collected from plants cultivated NIRP...
The mechanisms of response to radiation exposure are conserved in plants and animals. DNA damage (DDR) pathways the predominant molecular activated upon radiation, both features DDR animals might facilitate interdisciplinary studies that cross traditional boundaries between animal plant biology order expand collection biomarkers currently used for monitoring (REM) environmental biomedical settings. Genes implicated trans-kingdom networks often triggered by ionizing (IR) UV light deposited...
Summary paragraph In sexually propagating organisms, genetic and epigenetic mutations are evolutionarily relevant only if they occur in the germline provide inherited information to next generation. contrast most animals, plants thought lack an early segregating germline, implying that somatic cells can contribute progeny. Here we demonstrate two ARGONAUTE proteins, AGO5 AGO9, mark early-segregating germline. Both AGOs loaded with dynamically changing populations of small RNAs derived from...