A5014804872- Photosynthetic Processes and Mechanisms
- Antioxidant Activity and Oxidative Stress
- Plant responses to elevated CO2
- Algal biology and biofuel production
- Plant biochemistry and biosynthesis
- Phytochemicals and Antioxidant Activities
- Plant Stress Responses and Tolerance
- Phytochemistry and Biological Activities
- Plant Gene Expression Analysis
- Transgenic Plants and Applications
- Medicinal Plants and Bioactive Compounds
- Genomics and Phylogenetic Studies
- Biochemical and Structural Characterization
- Slime Mold and Myxomycetes Research
- RNA modifications and cancer
- Nanotechnology research and applications
- Graphene and Nanomaterials Applications
- Natural Antidiabetic Agents Studies
- PI3K/AKT/mTOR signaling in cancer
- Antimicrobial Peptides and Activities
- Pharmacology and Nanomedicine Research
- Plant Reproductive Biology
- Light effects on plants
- Microbial Metabolic Engineering and Bioproduction
- Nanoparticles: synthesis and applications
Hemwati Nandan Bahuguna Garhwal University
2023-2024
Max Planck Institute of Molecular Plant Physiology
2017-2022
Because carotenoids act as accessory pigments in photosynthesis, play a key photoprotective role and are of major nutritional importance, carotenogenesis has been target for crop improvement. Although important precursors phytohormones, previous genetic manipulations reported little if any effects on biomass production plant development, but resulted specific modifications carotenoid content. Unexpectedly, the expression carrot lycopene β-cyclase (DcLCYB1) Nicotiana tabacum cv. Xanthi not...
Antimicrobial peptides (AMPs) kill microbes or inhibit their growth and are promising next-generation antibiotics. Harnessing full potential as antimicrobial agents will require methods for cost-effective large-scale production purification. Here, we explore the possibility to exploit high protein synthesis capacity of chloroplast produce AMPs in plants. Generating a large series 29 sets transplastomic tobacco plants expressing nine different fusion proteins, show that high-level...
Abstract Carotenoids are important isoprenoids produced in the plastids of photosynthetic organisms that play key roles photoprotection and antioxidative processes. β-Carotene is generated from lycopene by β-cyclase (LCYB). Previously, we demonstrated introduction Daucus carota (carrot) DcLCYB1 gene into tobacco (cv. Xanthi) resulted increased levels abscisic acid (ABA) especially gibberellins (GAs), resulting plant yield. In order to understand this phenomenon prior exporting genetic...
Abstract The high-value carotenoid astaxanthin (3,3′-dihydroxy-β,β-carotene-4,4′-dione) is one of the most potent antioxidants in nature. In addition to its large-scale use fish farming, pigment has applications as a food supplement and an active ingredient cosmetics pharmaceuticals for treatment diseases linked reactive oxygen species. biochemical pathway synthesis been introduced into seed plants, which do not naturally synthesize this pigment, by nuclear plastid engineering. highest...
PsaI is the only subunit of PSI whose precise physiological function has not yet been elucidated in higher plants. While involved trimerization cyanobacteria, was lost during evolution eukaryotic PSI, and entire side underwent major structural remodelling to allow for binding light harvesting complex II antenna proteins state transitions. Here, we have generated a tobacco (Nicotiana tabacum) knockout mutant plastid-encoded psaI gene. We show that required redox reactions PSI. Neither...
Target of rapamycin (TOR) kinase is a sensor and central integrator internal external metabolic cues. However, in algae higher plants, the components TOR signaling are yet to be characterized. Here, we establish an assay system study activity Chlamydomonas reinhardtii using phosphorylation status its putative downstream target, CrS6K. Using this assay, probe modulation cellular under various physiological states such as photoautotrophy, heterotrophy, mixotrophy, nitrogen (N) starvation....
The chloroplast glutamyl-tRNA (tRNAGlu) is unique in that it has two entirely different functions. In addition to acting translation, serves as the substrate of reductase (GluTR), enzyme catalyzing committed step tetrapyrrole biosynthetic pathway. How tRNAGlu pool distributed between pathways and whether allocation limits biosynthesis and/or protein remains poorly understood. We generated a series transplastomic tobacco (Nicotiana tabacum) plants alter expression levels introduced point...
Picrorhiza kurroa Royle ex Benth., a medicinal plant native to the alpine regions of Himalayas, is renowned for its diverse therapeutic properties. Recently, green-synthesized silver nanoparticles have gained attention their potential in pharmaceutical and industrial applications. In this study, (Pk-AgNPs) were synthesized using commercially available root powder P. kurroa. The objective was evaluate biomedical applications by assessing antioxidant properties cytotoxicity. Pk-AgNPs...
Plants growing at high elevations experience different environmental stresses, such as drought, salt, and cold. Among them, cold stress is the most prevalent one that affects plants differently. undergo biochemical, metabolic, molecular, physiological changes under stress; hence, they adopt various mechanisms to tolerate it. The antioxidant defence system, osmotic regulators, photosynthetic pigments in plant provide them with tolerance. present study conducted on a high-altitude plant,...
ABSTRACT There are various plants that grow in high elevations and experience environmental stresses, cold stress being the most prevalent one. Plants undergo biochemical, metabolic, molecular, physiological changes under hence, they adopt mechanisms to tolerate it. The antioxidant defense system, osmotic regulators photosynthetic pigments plant provide them tolerance. present study is conducted on a high-altitude plant, Picrorhiza kurroa which grows such conditions, parameters provides it...
ABSTRACT T arget O f R apamycin (TOR) kinase is a sensor as well central integrator of internal and external metabolic cues. The upstream downstream signals this are very characterized in animals. However, algae, higher plants other photosynthetic organisms, the components TOR kinase-signaling yet to be characterized. Here, we establish an assay system study activity C.reinhardtii using phosphorylation status its target, CrS6K. We further use monitor under various physiological states such...
Picrorhiza kurroa Royle ex Benth is a high-altitude medicinal herb belonging to the family Scrophulariaceae, commonly called Kutki. It secretes various active constituents which impart pharmaceutical value plant. The roots and rhizomes of this plant are used for their health benefits since ancient times. Looking at endangered status species, present study was conducted on leaves kurroa. leaf samples were collected from two sites, Pothivasa (2200 masl) Tungnath (3600 masl), Rudraprayag,...