A5077598643- RNA modifications and cancer
- Metabolomics and Mass Spectrometry Studies
- Cancer, Hypoxia, and Metabolism
- Eicosanoids and Hypertension Pharmacology
- Epigenetics and DNA Methylation
- Histone Deacetylase Inhibitors Research
- Diet and metabolism studies
- Autophagy in Disease and Therapy
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Cancer, Lipids, and Metabolism
- RNA regulation and disease
- Metabolism, Diabetes, and Cancer
- Mass Spectrometry Techniques and Applications
- Coral and Marine Ecosystems Studies
- Pancreatic function and diabetes
- Acute Lymphoblastic Leukemia research
- Analytical Chemistry and Chromatography
- Sirtuins and Resveratrol in Medicine
- Mitochondrial Function and Pathology
- Evolution and Genetic Dynamics
- Phytoestrogen effects and research
- Gut microbiota and health
- Inflammatory mediators and NSAID effects
- Sulfur Compounds in Biology
- Cancer-related gene regulation
Rutgers, The State University of New Jersey
2017-2025
Rutgers Cancer Institute of New Jersey
2019-2025
Johnson University
2017-2025
Rutgers Sexual and Reproductive Health and Rights
2020-2025
Cancer Institute (WIA)
2024-2025
Robert Wood Johnson University Hospital
2025
Kunming Medical University
2020-2024
First Affiliated Hospital of Kunming Medical University
2020-2024
Cancer Institute of Florida
2023
Cornell University
2010-2019
Biological functions of sirtuins may involve lysine desuccinylase and demalonylase activities.
The investigation of intracellular metabolism is the mainstay in biotechnology and physiology settings. Intracellular metabolic rates are commonly evaluated using labeling pattern identified metabolites obtained from stable isotope experiments. or mass distribution vector describes fractional abundances all isotopologs with different masses as a result isotopic labeling, which typically resolved spectrometry. Because naturally occurring isotopes impurity also contribute to measured signals,...
Orbitraps are high-resolution ion-trap mass spectrometers that widely used in metabolomics. While the accuracy and resolving power of orbitraps have been extensively documented, their spectral accuracy, i.e., measuring abundances isotopic peaks, remains less studied. In analyzing spectra unlabeled metabolites, we discovered a systematic under representation heavier natural species, especially for high molecular weight metabolites (∼20% under-measurement [M + 1]/[M 0] ratio at m/z 600). We...
Highlights•GLUL expression is high in human pancreatic cancers and mouse PDAC models•aKG rescue of glutamine-deprived cells requires GLUL•De novo glutamine synthesis essential for nitrogen anabolic processes•Genetic ablation GLUL suppresses developmentSummaryGlutamine thought to play an important role cancer by being deaminated via glutaminolysis α-ketoglutarate (aKG) fuel the tricarboxylic acid (TCA) cycle. Supporting this notion, aKG supplementation can restore growth/survival cells....
Untargeted metabolomics can detect more than 10 000 peaks in a single LC–MS run. The correspondence between these and metabolites, however, remains unclear. Here, we introduce Peak Annotation Verification Engine (PAVE) for annotating untargeted microbial data. workflow involves growing cells 13C 15N isotope-labeled media to identify from biological compounds their carbon nitrogen atom counts. Improved deisotoping deadducting are enabled by algorithms that integrate positive mode, negative...
Loss of tumor suppressor liver kinase B1 (LKB1) promotes cancer cell proliferation but also leads to decreased metabolic plasticity in dealing with energy crises. Autophagy is a protective process involving self-cannibalization maintain cellular homeostasis during nutrient deprivation. We developed mouse model for Lkb1 -deficient lung conditional deletion essential autophagy gene Atg7 test whether compensates LKB1 loss cells survive found that ablation was synthetically lethal tumorigenesis...
Much understanding of metabolism is based on monitoring chemical reactions in cells with isotope tracers. For this purpose, 13C well suited due to its stable incorporation into biomolecules and minimal kinetic effect. redox reactions, deuterium tracing can provide additional information. To date, studies examining NADPH production deuterated carbon sources have failed account for roughly half NADPH's active hydrogen. We show the missing hydrogen result enzyme-catalyzed H-D exchange between...
Metabolomic profiling identifies small peptides that may provide early diagnosis of thermal stress and bleaching in coral.
Background: Hepatocellular Carcinoma (HCC) is a major form of liver cancer and leading cause cancer-related death worldwide. New insights into HCC pathobiology mechanism drug actions are urgently needed to improve patient outcomes. undergoes metabolic reprogramming glucose metabolism from respiration aerobic glycolysis, phenomenon known as the 'Warburg Effect' that supports rapid cell growth, survival, invasion. mTOR promote Warburg Effect, but underlying mechanism(s) remains poorly defined....
Butyrate (B) is a short-chain fatty acid produced by dietary fiber, known to inhibit histone deacetylases (HDACs) and possess cancer-preventive/anticancer effects. However, the role of B in metabolic rewiring, epigenomic reprogramming, transcriptomic network, NRF2 signaling, eliciting cancer-preventive effects colorectal cancer (CRC) HCT116 cell remains unclear.Sodium butyrate (NaB) dose-dependently inhibits growth CRC cells. NaB NRF2/NRF2-target genes blocks NRF2-ARE signaling. increases...
Abstract LKB1 and KRAS are the third most frequent co-mutations detected in non-small cell lung cancer (NSCLC) cause aggressive tumor growth. Unfortunately, treatment with RAS-RAF-MEK-ERK pathway inhibitors has minimal therapeutic efficacy LKB1-mutant KRAS-driven NSCLC. Autophagy, an intracellular nutrient scavenging pathway, compensates for Lkb1 loss to support Kras-driven Here we preclinically evaluate possibility of autophagy inhibition together MEK as a tumors. We found that combination...
Abstract Cancer cells depend on nicotinamide adenine dinucleotide phosphate (NADPH) to combat oxidative stress and support reductive biosynthesis. One major NADPH production route is the pentose pathway (committed step: glucose-6-phosphate dehydrogenase, G6PD). Alternatives exist can compensate in some tumors. Here, using genetically-engineered lung cancer mouse models, we show that G6PD ablation significantly suppresses Kras G12D/+ ;Lkb1 -/- (KL) but not ;P53 (KP) tumorigenesis. In vivo...
Decelerated degradation of beta-amyloid (Abeta) and its interaction with synaptic copper may be pathogenic in Alzheimer disease. Recently, Co(III)-cyclen tagged to an aromatic recognition motif was shown degrade Abeta vitro. Here, we report that apocyclen attached selective motifs (KLVFF or curcumin) can capture bound use the Cu(II) place Co(III) become proteolytically active. The resultant complexes interfere aggregation, into fragments, preventing H2O2 formation toxicity neuronal cell...