A5012061915- Extracellular vesicles in disease
- Phosphodiesterase function and regulation
- Mitochondrial Function and Pathology
- Receptor Mechanisms and Signaling
- Circular RNAs in diseases
- Cancer, Hypoxia, and Metabolism
- MicroRNA in disease regulation
- Neurological Disease Mechanisms and Treatments
- Adipose Tissue and Metabolism
- Adipokines, Inflammation, and Metabolic Diseases
- DNA Repair Mechanisms
- RNA Research and Splicing
- Retinal Development and Disorders
- Aquatic Ecosystems and Phytoplankton Dynamics
- Cancer-related molecular mechanisms research
- Protein Kinase Regulation and GTPase Signaling
- Melanoma and MAPK Pathways
- Cancer, Lipids, and Metabolism
- Bacteriophages and microbial interactions
- Selenium in Biological Systems
- Peroxisome Proliferator-Activated Receptors
- Cancer Mechanisms and Therapy
- Estrogen and related hormone effects
- Geomagnetism and Paleomagnetism Studies
- Renin-Angiotensin System Studies
Hunan Normal University
2024
Sun Yat-sen University
2017-2024
Sun Yat-sen University Cancer Center
2024
Anhui Provincial Hospital
2024
Chongqing University of Arts and Sciences
2020-2023
University of South China
2013-2023
Jinan University
2021-2023
Chiba University
2023
Second Affiliated Hospital of Zhejiang University
2023
Albert Einstein College of Medicine
2011-2022
Phosphorylated H2AX is considered to be a biomarker for DNA double-strand breaks (DSB), but recent evidence suggests that γH2AX does not always indicate the presence of DSB. Here we demonstrate bimodal dynamic phosphorylation induced by ionizing radiation, with second peak appearing when G2/M arrest induced. An increased level occurred in mitotic cells, and this increase was attenuated DNA-PKcs inactivation or Chk2 depletion, ATM inhibition. The phosphorylation-mimic CHK2-T68D abrogated...
Increased miR-375 levels has been implicated in rodent models of myocardial infarction (MI) and with patients heart failure. However, no prior study had established a therapeutic role ischemic myocardium. Therefore, we assessed whether inhibition MI-induced by LNA anti-miR-375 can improve recovery after acute MI. Ten weeks old mice were treated either control or anti induction MI LAD ligation. The inflammatory response, cardiomyocyte apoptosis, capillary density left ventricular (LV)...
Abstract Background Pancreatic cancer remains one of the most rapidly progressive and deadly malignancies worldwide. Current treatment regimens only result in small improvements overall survival for patients with this type. CPI-613 (Devimistat), a novel lipoate analog inhibiting mitochondrial metabolism, shows new hope pancreatic as an efficient well-tolerated therapeutic option treated alone or combination chemotherapy. Methods cells growing planar 2D cultures 3D scaffold were used research...
Abstract Background Enolase 2 (ENO2) is a crucial glycolytic enzyme in cancer metabolic process and acts as “moonlighting” protein to play various functions diverse cellular processes unrelated glycolysis. ENO2 highly expressed head neck squamous cell carcinoma (HNSCC) tissues relative normal tissues; however, its impact underlying regulatory mechanisms HNSCC malignancy remain unclear. Methods Molecular alterations were examined by bioinformatics, qRT-PCR, western blotting,...
Mechanisms to maintain genomic integrity are essential for cells remain viable. Not surprisingly, disruption of key DNA damage response pathway factors, such as ataxia telangiectasia-mutated (ATM)/ataxia telangiectasia and RAD3-related (ATR) results in loss integrity. Here, a synthetic lethal siRNA-screening approach not only confirmed ATM but identified additional replication checkpoint proteins, when ablated, enhanced ATR inhibitor (ATRi) high-content γ-H2AX assay. Cancers with...
Previous studies have demonstrated that mice fed a high-fat diet (HFD) develop insulin resistance with proinflammatory macrophage infiltration into white adipose tissue. Concomitantly, adipocytes undergo programmed cell death the loss of adipocyte-specific lipid droplet protein perilipin, and dead/dying are surrounded by macrophages organized crown-like structures. This study investigated whether adipocyte provides driving signal for inflammation or if induces death.
Adipose tissue inflammation is one pathway shown to mediate insulin resistance in obese humans and rodents. Obesity induces dynamic cellular changes adipose increase proinflammatory cytokines diminish anti-inflammatory cytokines. However, we have found that interleukin-13 (IL-13) unexpectedly induced of high-fat diet (HFD)-fed mice, the source IL-13 primarily adipocyte. Moreover, HFD-induced such as tumor necrosis factor alpha (TNF-α) IL-1β production adipocytes an IKKβ-dependent manner. In...
Abstract Self‐renewal and differentiation of spermatogonial stem cell (SSC) are critical for male fertility reproduction, both which highly regulated by testicular microenvironment. Exosomal miRNAs have emerged as new components in intercellular communication. However, their roles the SSC remain unclear. Here, we observed miR‐486‐5p enriched Sertoli cell‐derived exosomes. The exosomes mediate transfer from cells to SSCs. Exosomes release miR‐486‐5p, thus up‐regulate expression Stra8...
Increasing evidence from experimental research suggests that exposure to microcystins (MCs) may induce lipid metabolism disorder. However, population-based epidemiological studies of the association between MCs and risk dyslipidemia are lacking. Therefore, we conducted a cross-sectional study involving 720 participants in Hunan Province, China, evaluated effects on blood lipids. After adjusting related metals, used binary logistic regression multiple linear models examine associations among...
Organochlorine compounds have been demonstrated to detrimental health effects in both wildlife and humans, an effect largely attributed their ability mimic the hormone estrogen. Our laboratory has studied cell signaling by environmental chemicals associated with estrogen receptor (ER) more recently via ER-independent mechanisms. Here, we show that organochlorine pesticide dichlorodiphenyltrichloroethane (DDT) its metabolites induce a stress mitogen-activated protein kinase (MAPK) leads AP-1...
In adipocytes, PDE3B (phosphodiesterase 3B) is an important regulatory effector in signalling pathways controlled by insulin and cAMP-increasing hormones. Stimulation of 3T3-L1 adipocytes with or the β3-adrenergic receptor agonist CL316243 (termed CL) indicated that preferentially phosphorylated/activated associated internal membranes (endoplasmic reticulum/Golgi), whereas CL caveolae. siRNA (small interfering RNA)-mediated KD (knockdown) CAV-1 (caveolin-1) resulted down-regulation...
Endocrine resistance and metastatic progression are primary causes of treatment failure in breast cancer. While mitogen activated protein kinases (MAPKs) known to promote ligand-independent cell growth, the role MEK5-ERK5 pathway clinical carcinoma remains poorly understood. Here, we demonstrated increased ERK5 activation 30 39 (76.9%) tumor samples, as well across cancer systems. Overexpression MEK5 MCF-7 cells promoted both hormone-dependent hormone-independent tumorigenesis vitro vivo...
Abstract Activation of inflammation in white adipose tissue (WAT), includes infiltration/expansion WAT macrophages, contributes pathogenesis obesity, insulin resistance and metabolic syndrome. The inflammasome comprises an intracellular sensor (NLR), caspase-1 the adaptor ASC. Inflammasome activation leads to maturation processing IL1β, contributing many disorders directing adipocytes a more insulin-resistant phenotype. Ablation PDE3B prevents by reducing expression NLRP3, caspase-1, ASC,...
The ovary is a highly susceptible organ to senescence, and granulosa cells (GCs) have crucial role in oocyte development promotion overall ovarian function maintenance. As age advances, GCs apoptosis dysfunction escalate, leading aging. However, the molecular mechanisms underpinning aging remain poorly understood. In this study, we observed correlation between age-related decline of fertility elevated expression levels miR-143-3p female mice. Moreover, was expressed senescent GCs....
Fractionation of 3T3-L1 adipocyte membranes revealed that PDE3B (phosphodiesterase 3B) was associated with PM (plasma membrane) and ER (endoplasmic reticulum)/Golgi fractions, insulin-induced phosphorylation/activation greater in internal than there no significant translocation between membrane fractions. Insulin also induced formation large macromolecular complexes, separated during gel filtration (Superose 6 columns) solubilized membranes, which apparently contain phosphorylated/activated...
Chronic heart failure (CHF) is a prevalent health concern with complex pathogenesis. This current study set out to estimate the function of miR-129-5p/Smurf1/PTEN axis on cardiac injury in CHF. The model CHF rats was established. indexes, myocardial tissue damage, and oxidative stress-related factors were evaluated after interference Smurf1/miR-129-5p/PTEN. targeting relationships between miR-129-5p Smurf1 PTEN verified. It found that modeling, functions impaired, heart/left ventricular/lung...
We have investigated greigite (Fe 3 S 4 ) using a combination of neutron powder diffraction and polarized to give the first unambiguous assignment its magnetic structure. Our results confirm that has collinear ferrimagnetic structure with antiferromagnetic coupling between tetrahedral ( A octahedral B sites. analysis also indicates does not significant vacancy concentration or spin canting. High‐resolution enable determination sublattice magnetizations At room temperature, average moments on...