A5100628844- Cancer-related molecular mechanisms research
- Circular RNAs in diseases
- DNA Repair Mechanisms
- Neuroscience and Neuropharmacology Research
- Advanced biosensing and bioanalysis techniques
- MicroRNA in disease regulation
- Cancer-related gene regulation
- RNA modifications and cancer
- Cellular transport and secretion
- Genetic Neurodegenerative Diseases
- Extracellular vesicles in disease
- RNA Research and Splicing
- PARP inhibition in cancer therapy
- Epigenetics and DNA Methylation
- RNA Interference and Gene Delivery
- Angiogenesis and VEGF in Cancer
- Photoreceptor and optogenetics research
- Cancer therapeutics and mechanisms
- Advanced Nanomaterials in Catalysis
- Analytical chemistry methods development
- Endoplasmic Reticulum Stress and Disease
- Genetics, Bioinformatics, and Biomedical Research
- Peroxisome Proliferator-Activated Receptors
- Autophagy in Disease and Therapy
- Cancer-related Molecular Pathways
Wuhan Polytechnic University
2022-2025
Max Planck Florida Institute for Neuroscience
2022-2025
Peking University
2020-2025
Peking University Third Hospital
2020-2025
Xuzhou Medical College
2019-2025
Guangzhou Medical University
2021-2025
Hunan University of Traditional Chinese Medicine
2021-2025
University of Southern California
2023-2024
Fourth Affiliated Hospital of China Medical University
2022-2023
University of California, San Francisco
2023
Phosphorylated H2AX is considered to be a biomarker for DNA double-strand breaks (DSB), but recent evidence suggests that γH2AX does not always indicate the presence of DSB. Here we demonstrate bimodal dynamic phosphorylation induced by ionizing radiation, with second peak appearing when G2/M arrest induced. An increased level occurred in mitotic cells, and this increase was attenuated DNA-PKcs inactivation or Chk2 depletion, ATM inhibition. The phosphorylation-mimic CHK2-T68D abrogated...
Mitochondrial fission promotes glioma progression. The function and regulation mechanisms of lncRNAs in mitochondrial are unclear. expression LINC00475 its correlation with clinical parameters were analyzed using bioinformatics. Then, vitro vivo assays performed to explore the spliced variant (LINC00475-S) gliomas. To mechanisms, RNA-seq, MeRIP, RIP, pulldown-IP, dCas9-ALKBH5 editing system, LC/MS, Western blotting utilized. was confirmed be overexpressed higher frequencies AS events gliomas...
The SEL1L-HRD1 protein complex represents the most conserved branch of endoplasmic reticulum (ER)-associated degradation (ERAD). Despite recent advances in both mouse models and humans, vivo evidence for importance SEL1L ERAD formation its (patho-)physiological relevance mammals remains limited. Here we report that variant p.Ser658Pro (SEL1L
The main objective of this study is to evaluate the influence exosomes derived from endothelial progenitor cells (EPC-Exo) on neointimal formation induced by balloon injury in rats. Furthermore, aims investigate potential EPC-Exo promote proliferation, migration, and anti-apoptotic effects vascular (VECs) vitro. underlying mechanisms responsible for these observed will also be thoroughly explored analyzed. Endothelial (EPCs) was isolated aseptically Sprague-Dawley (SD) rats cultured complete...
An increasing number of studies have recently reported that microRNAs packaged in exosomes contribute to multiple biological processes such as cancer progression; however, little is known about their role the development radiation-induced bystander effects.The were isolated from culture medium BEP2D cells with or without γ-ray irradiation by ultracentrifugation. To monitor DNA damage and repair efficiency, double-strand break biomarker 53BP1 foci, comet, micronuclei, expression genes NHEJ...
Dysfunctions of gene transcription and translation in the nociceptive pathways play critical role development maintenance chronic pain. Circular RNAs (circRNAs) are emerging as new players regulation expression, but whether how circRNAs involved pain remain elusive. We showed here that complete Freund's adjuvant-induced inflammation significantly increased circRNA-Filip1l (filamin A interacting protein 1-like) expression spinal neurons mice. Blockage this increase attenuated behaviors,...
Ribosomal proteins are highly conserved components of basal cellular organelles, primarily involved in the translation mRNA leading to protein synthesis. However, certain ribosomal moonlight development and differentiation organisms. In this study, L44 (RPL44), associated with salt resistance, was screened from halophilic fungus Aspergillus glaucus (AgRPL44), its activity investigated Saccharomyces cerevisiae Nicotiana tabacum. Sequence alignment revealed that AgRPL44 is one large subunit...
Abstract The methyltransferase-like 3 ( Mettl3 ) is a key component of the large N6-adenosine-methyltransferase complex in mammalian responsible for RNA N6-methyladenosine (m 6 A) modification, which plays an important role gene post-transcription modulation. Although m A enriched neurons, its regulatory function nociceptive information processing remains elusive. Here, we reported that Complete Freund's Adjuvant (CFA)-induced inflammatory pain significantly decreased global level and writer...
The insulin superfamily of peptides is essential for homeostasis as well neuronal plasticity, learning, and memory. Here, we show that insulin-like growth factors 1 2 (IGF1 IGF2) are differentially expressed in hippocampal neurons released an activity-dependent manner. Using a new fluorescence resonance energy transfer sensor IGF1 receptor (IGF1R) with two-photon lifetime imaging, find the release triggers rapid local autocrine IGF1R activation on same spine more than several micrometers...
Abstract End resection of DNA double-strand breaks (DSBs) to form 3′ single-strand (ssDNA) is critical initiate the homologous recombination (HR) pathway DSB repair. HR strictly limited in G1-phase cells because lack as templates. Exonuclease 1 (EXO1) key molecule responsible for ssDNA formation end resection. We revealed that EXO1 inactivated via ubiquitination-mediated degradation, resulting from an elevated expression level RING-box protein (RBX1) G1 phase. The increased RBX1...
The role of circ_0089153 in breast cancer (BCa) malignancy development was explored. expression BCa analyzed by Gene Expression Omnibus database. Clinical tissues were obtained from 90 patients. Cell counting kit-8 assay, 5-ethnyl-2 deoxyuridine assay and colony formation experiment applied for proliferation detection. Wound healing Transwell used migration invasion Dual luciferase reporter gene RNA immunoprecipitation pull-down conducted. In vivo growth metastasis cells performed....
Abstract Sonic Hedgehog (SHH) subgroup of medulloblastoma (MB) accounts for about 25% all subgroups MB. Tumor microenvironment (TME) may play a key role in the tumor progression and therapeutic resistance. Tumor‐associated astrocytes (TAAs) are reshaped to drive through multiple paracrine signals. However, mechanism by which TAAs modulate MB cells remains elusive. Here, we illuminated that showed specific dynamic pattern during SHH‐MB development. Most gathered margin progression, rather...
Abstract Background Medulloblastoma (MB) is the most common pediatric malignant brain tumor. Infiltration of tumor-associated macrophages (TAMs) and m6A modification RNA are correlated with poor prognosis tumor progression in Sonic Hedgehog (SHH) subtype (SHH-MB). However, relationship between TAMs infiltration SHH-MB status during remains unclear. Methods Expression modification-related proteins was assessed 40 cases SHH-MB. Genes affected by TAM-derived exosomes were identified methylated...
Radiation therapy, one of the major treatments for liver cancer, causes DNA damage and cell death. Since cancer cells have a strong capacity to repair irradiative injury, new medicines enhance this treatment are urgently required. In study, we investigated effect NU7441, synthetic small-molecule compound, as specific inhibitor DNA-dependent protein kinase (DNA-PK) in radiosensitization hepatocellular carcinoma HepG2 cells.Cell Counting Kit-8 (CCK-8) was first used evaluate proliferation...
DNA-dependent protein kinase catalytic subunit (DNA-PKcs) is the core component of DNA-PK complex in non-homologous end-joining (NHEJ) repair DNA double-strand breaks, and its activity strictly controlled by DNA-PKcs phosphorylation. The ubiquitin-like protein, NEDD8 involved regulation damage response, but it remains mysterious whether how NEDD8-related neddylation affects NHEJ process. Here, we show that poly-neddylated at domain. E2-conjugating enzyme UBE2M E3 ligase HUWE1 (HECT, UBA, WWE...
Nonhomologous end joining (NHEJ) and homologous recombination (HR) are major repair pathways of DNA double-strand breaks (DSBs). The pathway choice HR NHEJ is tightly regulated in cellular response to damage. Here, we demonstrate that the interaction TIP60 with DNA-PKcs attenuated specifically S phase, which facilitates activation. SUMO2 modification K430 mediated by PISA4 E3 ligase blocks its DNA-PKcs, whereas K430R mutation recovers results abnormally increased phosphorylation S2056 phase...
Chronic inflammatory pain seriously affects patients’ quality of life because a paucity effective clinical treatments caused, at least in part, by lack full understanding the underlying mechanisms. miRNAs are known to be involved via silencing or degrading target mRNA cytoplasm. The present study provides novel mechanism which miRNA-22 positively regulates metal-regulatory transcription factor 1 (Mtf1) nuclei neurons dorsal horn spinal cord. We found that was significantly increased mice...
The effects of cinnamaldehyde microcapsules on the concentration and its metabolites in plasma, urine, feces, antioxidant capacity, intestinal flora male C57/BL6 mice were evaluated by oral administration for 7 weeks. Microencapsulation significantly increased contents cinnamaldehyde, cinnamyl alcohol, methyl cinnamate plasma decreased those urine feces excretion (p < 0.05). In addition, microencapsulated improved capacity liver, duodenum, colon. Furthermore, 16S rRNA gene sequencing data...