A5009972760- Glycosylation and Glycoproteins Research
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Advanced Proteomics Techniques and Applications
- Carbohydrate Chemistry and Synthesis
- Peptidase Inhibition and Analysis
- Metabolism, Diabetes, and Cancer
- Bioinformatics and Genomic Networks
- Growth Hormone and Insulin-like Growth Factors
- Ubiquitin and proteasome pathways
- RNA modifications and cancer
- Genomics and Chromatin Dynamics
- Cancer, Hypoxia, and Metabolism
- S100 Proteins and Annexins
- Ovarian cancer diagnosis and treatment
- TGF-β signaling in diseases
- Lipid metabolism and disorders
- Proteoglycans and glycosaminoglycans research
- 14-3-3 protein interactions
- Machine Learning in Bioinformatics
- Caveolin-1 and cellular processes
- Cancer-related molecular mechanisms research
- Gastric Cancer Management and Outcomes
- Barrier Structure and Function Studies
- Protein Degradation and Inhibitors
Macquarie University
2012-2017
The University of Sydney
1998-2011
Children's Hospital at Westmead
2004-2011
Molecular Oncology (United States)
2008
Royal North Shore Hospital
1998-2002
Advances in colorectal cancer (CRC) diagnosis will be enhanced by development of more sensitive and reliable methods for early detection the disease when treatment is effective. Because many known biomarkers are membrane-bound glycoproteins with important biological functions, we chose to compare N-glycan profiles membrane proteins from three phenotypically different CRC cell lines, LIM1215, LIM1899, LIM2405, representing moderately differentiated metastatic, primary, poorly (aggressive)...
Glycomics may assist in uncovering the structure–function relationships of protein glycosylation and identify glycoprotein markers colorectal cancer (CRC) research. Herein, we performed label-free quantitative glycomics on a carbon-liquid chromatography–tandem mass spectrometry-based analytical platform to accurately profile N-glycosylation changes associated with CRC malignancy. N-Glycome profiling was isolated membrane proteomes paired tumorigenic adjacent non-tumorigenic colon tissues...
We previously demonstrated in T47D cells transfected to express the transforming growth factor-beta receptor type II (TGF-betaRII) that insulin-like factor binding protein-3 (IGFBP-3) could stimulate Smad2 and Smad3 phosphorylation, potentiate TGF-beta1-stimulated Smad cooperate with exogenous TGF-beta1 cell inhibition (Fanayan, S., Firth, S. M., Butt, A. J., Baxter, R. C. (2000) J. Biol. Chem. 275, 39146-39151). This study further explores IGFBP-3 signaling through pathway. Like TGF-beta1,...
Glycoproteins perform extra- and intracellular functions in innate adaptive immunity by lectin-based interactions to exposed glyco-determinants. Herein, we document mechanistically explain the formation of subcellular-specific N-glycosylation determinants on glycoproteins trafficking through shared biosynthetic machinery human cells. LC-MS/MS-based quantitative glycomics showed that secreted eight breast epithelial cells displaying diverse geno- phenotypes consistently displayed more...
Abstract Purpose: Tumor protein D52 (TPD52 or D52) is frequently overexpressed in breast and other cancers present at increased gene copy number. It is, however, unclear whether amplification overexpression target specific functional properties of the encoded protein. Experimental Design: The expression D52-like genes MAL2 was compared tissues using quantitative reverse transcription-PCR. functions human D53 were then by stable BALB/c 3T3 fibroblasts transient knockdown carcinoma cell lines....
The four-transmembrane MAL2 protein is frequently overexpressed in breast carcinoma, and overexpression associated with gain of the corresponding locus at chromosome 8q24.12. Independent expression microarray studies predict ovarian but these had remained unconfirmed. binds tumor D52 (TPD52), which clinical significance TPD52 was unknown. Immunohistochemical analyses were performed using tissue sections including benign, borderline malignant epithelial tumours. Inmmunohistochemical staining...
In this study we selected three breast cancer cell lines (SKBR3, SUM149 and SUM190) with different oncogene expression levels involved in ERBB2 EGFR signaling pathways as a model system for the evaluation of selective integration subsets transcriptomic proteomic data. We assessed status reads per kilobase million mapped (RPKM) values (14.4, 400, 300 SUM149, SUM190, SKBR3, respectively) (60.1, not detected, 1.4 same 3 lines). then used RNA-Seq data to identify those oncogenes significant...
Abstract Background The MAL2 gene, encoding a four-transmembrane protein of the MAL family, is amplified and overexpressed in breast other cancers, yet significance this unknown. MAL-like proteins have trafficking functions, but their molecular roles are largely obscure, partly due to lack known binding partners. Methods Yeast two-hybrid screening carcinoma cDNA expression library was performed using full-length bait, subsequent deletion mapping experiments were performed. interactions...
We report progress assembling the parts list for chromosome 17 and illustrate various processes that we have developed to integrate available data from diverse genomic proteomic knowledge bases. As primary resources, used GPMDB, neXtProt, PeptideAtlas, Human Protein Atlas (HPA), GeneCards. All sites share common resource of Ensembl genome modeling information. defined with following information: 1169 protein-coding genes, numbers proteins confidently identified by experimental approaches as...
The secreted cellular sub-proteome (secretome) is a rich source of biologically active glycoproteins. N-Glycan profiling secretomes cultured cancer cells provides an opportunity to investigate the link between protein N-glycosylation and tumorigenesis. Utilizing carbon-LC–ESI-CID-MS/MS released native N-glycans, we accurately profiled secretome six human epithelial breast including normal mammary (HMEC) belonging luminal A subtype (MCF7), HER2-overexpressing (SKBR3), basal B (MDA-MB157,...
As part of the genome-wide and chromosome-centric human proteomic project (C-HPP), we have integrated shotgun proteomics approach a transcriptomic (RNA-Seq) set colon cancer cell lines (LIM1215, LIM1899 LIM2405) that were selected to represent wide range pathological states colorectal cancer. The combination standard (1D-gel electrophoresis coupled LC/ion trap mass spectrometry) RNA-Seq allowed us exploit greater depth transcriptomics measurement (∼ 9800 transcripts per line) versus protein...
Medulloblastoma (MB) is the most common malignant pediatric brain tumor. Patient survival has remained largely same for past 20 years, with therapies causing significant health, cognitive, behavioral and developmental complications those who survive In this study, we profiled total transcriptome proteome of two established MB cell lines, Daoy UW228, using high-throughput RNA sequencing (RNA-Seq) label-free nano-LC-MS/MS-based quantitative proteomics, coupled advanced pathway analysis. While...
Lectins are capable of recognizing specific glycan structures and serve as invaluable tools for the separation glycosylated proteins from nonglycosylated in biological samples. We report on optimization native multi‐lectin affinity chromatography, combining three lectins, namely, concanavalin A , jacalin, wheat germ agglutinin fractionation cellular glycoproteins MCF ‐7 breast cancer lysate. evaluated several conditions optimum recovery total such low pH saccharide elution buffers, inclusion...
In this manuscript, we describe a shotgun proteomics approach for comprehensive proteomic analysis of samples including total lysates, membrane, secretome, and exosome fractions from panel colorectal cancer cell lines. We will present an our data in two alternative formats. First discuss traditional data, which identify number cancer-associated proteins using various tools. second approach, use chromosome format to organize the on 7, allowing identification clusters genes with boundaries...