A5103150355- Silkworms and Sericulture Research
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- RNA Research and Splicing
- Neurobiology and Insect Physiology Research
- Silk-based biomaterials and applications
- Insect Resistance and Genetics
- Genomics and Chromatin Dynamics
- Reproductive Biology and Fertility
- Epigenetics and DNA Methylation
- Insect and Arachnid Ecology and Behavior
- Animal Behavior and Reproduction
- Insect Utilization and Effects
- Pluripotent Stem Cells Research
- Viral Infectious Diseases and Gene Expression in Insects
- Insect and Pesticide Research
- Animal Genetics and Reproduction
- CRISPR and Genetic Engineering
- Chromosomal and Genetic Variations
- Entomopathogenic Microorganisms in Pest Control
- Insect Pheromone Research and Control
- RNA modifications and cancer
- Invertebrate Immune Response Mechanisms
- Carcinogens and Genotoxicity Assessment
- DNA Repair Mechanisms
- Plant Molecular Biology Research
The University of Tokyo
2014-2024
Suzuki (Japan)
2022
Kobe University
2022
Grieg Seafood (Norway)
2019
Chubu University
2018
RIKEN
1999-2013
Ube Frontier University
2011
Tokyo University of Agriculture and Technology
1994-2004
Hosei University
2004
Institute of Agrobiological Sciences
2003
In mice, transcription initiates at the mid-one-cell stage and transcriptional activity dramatically increases during two-cell stage, a process called zygotic gene activation (ZGA). Associated with ZGA is marked change in pattern of expression that occurs after second round DNA replication. To distinguish before second-round replication, former latter are minor major ZGA, respectively. Although required for development beyond function not well understood. Transiently inhibiting 5,...
To build a foundation for the complete genome analysis of Bombyx mori , we have constructed an EST database. Because gene expression patterns deeply depend on tissues as well developmental stages, analyzed many cDNA libraries prepared from various and different stages to cover entire set genes. So far, database contains 35,000 ESTs 36 libraries, which are grouped into ≈11,000 nonredundant with average length 1.25 kb. The comparison FlyBase suggests that present database, SilkBase, covers...
The establishment of a complete genomic sequence silkworm, the model species Lepidoptera, laid foundation for its functional genomics. A more annotation genome will benefit and comparative studies accelerate extensive industrial applications this insect. To realize these goals, we embarked upon large-scale full-length cDNA collection from 21 libraries derived 14 tissues domesticated silkworm performed full sequencing by primer walking 11,104 cDNAs. large average intron size was 1904 bp,...
The straight-chain C 10 to 18 unsaturated aliphatic compounds containing an oxygenated functional group (aldehyde, alcohol, or acetate ester) derived from saturated 16 fatty acids are a major class of sex pheromone components produced by female moths. In the biosynthesis these components, various combinations limited chain-shortening and regio- stereospecific desaturation reactions significantly contribute production vast number species-specific in Lepidoptera. Biosynthesis silkmoth...
In most Lepidoptera, pheromone biosynthesis is regulated by a neuropeptide termed activating (PBAN). Although much known about the cellular targets of PBAN, identification and functional characterization PBAN receptor (PBANR) has proven to be elusive. Given sequence similarity between active C-terminal regions neuromedin U, it was hypothesized that their respective receptors might also similar in structure (Park, Y., Kim, Y. J., Adams, M. E. (2002) Proc. Natl. Acad. Sci. U. S. A. 99,...
Summary The sex determination pathway is different between Drosophila melanogaster and Bombyx mori in the initial signal. Here we show evidence that B. similar to of D. at level terminal regulator, doublesex ( dsx ), which essential for proper differentiation sexually dimorphic somatic features . In , a homolog Bmdsx ) expressed various tissues, its primary transcript alternatively spliced males females yield sex‐specific mRNAs encode male‐specific (BmDSXM) female‐specific (BmDSXF)...
Bmdsx is a sex-determining gene in the silkworm and alternatively spliced males females. CE1 splicing silencer element responsible for sex-specific of Bmdsx. To identify factors implicated Bmdsx, we performed RNA affinity chromatography using as ligand. We have identified BmIMP, Bombyx homolog IGF-II mRNA binding protein (IMP), male-specific factor that specifically binds to CE1. The encoding BmIMP localized on Z chromosome male-specifically expressed various tissues. Antisense inhibition...
Zygotes are totipotent cells that have the ability to differentiate into all cell types. It is believed this lost gradually and differentiation occurs along with progression of preimplantation development. Here, we hypothesized loose chromatin structure involved in totipotency one-cell stage embryos change from tight associated loss totipotency. To address hypothesis, investigated mobility eGFP-tagged histone H2B (eGFP-H2B), which an index for looseness chromatin, during development based on...
The WZ sex determination system is found in a diverse range of animals, including lepidopteran insects. In the silkworm Bombyx mori, model insect, females carry W chromosome with feminizing gene Feminizer (Fem), which source female-specific PIWI-interacting RNA (piRNA). Fem piRNA-PIWI complex cleaves an mRNA encoding masculinizing protein Masculinizer (BmMasc), resulting production female-type splice variant mori doublesex (Bmdsx), master genetic switch B. differentiation. contrast, males,...
ABSTRACT The baculovirus Bombyx mori nucleopolyhedrovirus (BmNPV) contains five related open reading frames (ORFs). Recent sequence analyses of several other genomes reveal that these ORFs belong to a unique multigene family called the repeated ( bro ) family. Here we have characterized genes from BmNPV at transcriptional and translational levels. Reverse transcription-PCR primer extension indicated transcription all occurs by 2 4 h postinfection (p.i.) reaches maximal levels between 8 12...
The Bombyx mori homolog of doublesex, Bmdsx, plays an essential role in silkworm sexual development. Exons 3 and 4 Bmdsx pre-mRNA are specifically excluded males. To explore how this occurs, we developed a novel vivo sex-specific splicing assay system using sexually differentiated cultured cells. A series mutation analyses minigene with identified three distinct sequences (CE1, CE2, CE3) positioned exon as exonic silencers responsible for male-specific splicing. Gel shift analysis showed...
In <I>Drosophila melanogaster, transformer-2 (tra-2)</I> is essential for female differentiation and known to induce female-specific splicing of <I>doublesex (dsx)</I>. The function <I>Bmtra-2</I>, the <I>Bombyx mori</I> homolog <I>tra-2</I>, on other hand remains be elucidated. As an initial step learn about biological we determined whether <I>Bmtra-2</I> capable inducing dsx</I>. RNAi-mediated knockdown using <I>Bombyx</I> cultured cells transiently transfected with a <I>dsx</I> minigene...
In Bombyx mori (B. mori), Fem piRNA originates from the W chromosome and is responsible for femaleness. The piRNA-PIWI complex targets cleaves mRNAs transcribed Masc gene. encodes a novel CCCH type zinc-finger protein required male-specific splicing of B. doublesex (Bmdsx) transcripts. present study, several silkworm strains carrying transgene, which piRNA-resistant mRNA (Masc-R), were generated. Forced expression Masc-R transgene caused female-specific lethality during larval stages. One...
As mouse oocytes approach maturity, a global repression of gene transcription occurs. Here, we investigated the involvement RPB1, largest subunit RNA polymerase II (RNAP II), in regulation this transcriptional silencing mechanism. Using BrUTP to follow an vitro run-on assay, observed abrupt decrease activity when reached their full size (approximately 80 µm). Immunoblotting using antibodies specific for phosphorylated and unphosphorylated forms RPB1 revealed that is at Ser-2 Ser-5 small...
The tightly condensed chromatin of spermatozoa is rapidly decondensed after the enter oocytes. Although no factor involved in sperm decondensation (SCD) has been identified mammals, it suggested that a related to SCD activity present germinal vesicle (GV) Here, we found nucleolus-like body (NLB), which component GV, murine When NLBs were microsurgically removed from GV-stage oocytes, was significantly retarded paternal genome fertilization following meiotic maturation. We retardation...
The genome of differentiated somatic nuclei is remodeled to a totipotent state when they are transplanted into enucleated oocytes. To clarify the mechanism this remodeling, we analyzed changes in composition core histone variants nuclear-transferred embryos, since recent evidence has revealed that chromatin structure can be as result variant replacement. We found donor cell-derived H3 H3.1, H3.2, and H3.3, well H2A H2A.Z, were rapidly eliminated from Accompanying removal, oocyte-stored H2A.X...
The global chromatin configuration is dramatically remodeled during fertilization and early preimplantation development. Although the chromocenters, which are pericentromeric heterochromatin clusters, observed in nuclei of oocytes, they disappear after then reappear at four-cell stage. To elucidate mechanism this reorganization heterochromatin, we investigated expression nuclear localization DOT1L, involved regulation structure through histone H3 lysine 79 (H3K79) methyltransferase activity,...
Sexual fate of the sawfly, Athalia rosae (Hymenoptera: Tenthredinidae) is determined by complementary sex determination (CSD) mechanism as case in honeybees. However, to date, genes involved have not been identified this species. In study, we attempted identify orthologs sex-determiner (csd), feminizer (fem), and doublesex (dsx) from A. genome, all which are crucial components cascade honeybee. As a result, sawfly ortholog dsx (designated Ardsx). Rapid amplification cDNA ends (RACE) using...