A5074861169- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Insect symbiosis and bacterial influences
- Entomopathogenic Microorganisms in Pest Control
- Insect and Pesticide Research
- Virus-based gene therapy research
- Mosquito-borne diseases and control
- Invertebrate Immune Response Mechanisms
- Biochemical Analysis and Sensing Techniques
- Neurobiology and Insect Physiology Research
- Insect-Plant Interactions and Control
- thermodynamics and calorimetric analyses
- Viral Infections and Immunology Research
- Liver Disease Diagnosis and Treatment
- Phytochemicals and Antioxidant Activities
- Plant tissue culture and regeneration
- Genomics and Phylogenetic Studies
- Plant biochemistry and biosynthesis
- Biochemical and biochemical processes
- Endoplasmic Reticulum Stress and Disease
- Protein Degradation and Inhibitors
- Gene expression and cancer classification
- Molecular Biology Techniques and Applications
- Liver Diseases and Immunity
- Silk-based biomaterials and applications
The University of Tokyo
2020-2025
Meiji University
2016
RIKEN
2003-2011
Laboratory of Racing Chemistry
2005-2011
Tokyo University of Agriculture and Technology
2000
Mukogawa Women's University
1982
Bacterial symbionts, such as Wolbachia species, can manipulate the sexual development and reproduction of their insect hosts. For example, infection induces male-specific death in Asian corn borer Ostrinia furnacalis by targeting host factor Masculinizer (Masc), an essential protein for masculinization dosage compensation lepidopteran insects. Here we identify a protein, designated Oscar, which interacts with Masc via its ankyrin repeats. Embryonic expression Oscar inhibits Masc-induced...
Parasitoid wasps, one of the most diverse and species-rich animal groups on Earth, produce venoms that manipulate host development physiology to exploit resources. However, mechanisms actions these remain poorly understood. Here, we discovered endoparasitoid wasp, Asobara japonica , induces apoptosis, autophagy, mitotic arrest in adult tissue precursors its Drosophila larvae. We termed this phenomenon imaginal disc degradation (IDD). A multi-omics approach facilitated identification two...
Wolbachia are maternally transmitted bacterial symbionts that ubiquitous among arthropods. They can hijack host reproduction in various ways, including male killing (MK), where the sons of infected mothers killed during development. The recent discovery MK-associated genes, i.e., oscar Ostrinia moths and wmk Drosophila flies, stimulates our interest diversity commonality MK mechanisms, which remain largely unclear. We recently discovered a symbiont moth Homona magnanima carries an prophage...
The WZ sex determination system is found in a diverse range of animals, including lepidopteran insects. In the silkworm Bombyx mori, model insect, females carry W chromosome with feminizing gene Feminizer (Fem), which source female-specific PIWI-interacting RNA (piRNA). Fem piRNA-PIWI complex cleaves an mRNA encoding masculinizing protein Masculinizer (BmMasc), resulting production female-type splice variant mori doublesex (Bmdsx), master genetic switch B. differentiation. contrast, males,...
Wolbachia are maternally transmitted bacterial symbionts that ubiquitous among arthropods. They can hijack host reproduction in various ways, including male killing (MK), where the sons of infected mothers killed during development. The recent discovery MK-associated genes, i.e., oscar Ostrinia moths and wmk Drosophila flies, stimulates our interest diversity commonality MK mechanisms, which remain largely unclear. We recently discovered a symbiont moth Homona magnanima carries an prophage...
ABSTRACT Alphabaculoviruses produce a large number of occlusion bodies (OBs) in host cells during the late stage infection. OBs are mainly composed viral product polyhedrin (POLH), and extremely high-level transcription polh gene has been exploited to express foreign proteins insect cultured cells, larvae, pupae. This hyper-transcription requires “burst sequence” located between transcriptional start site initiation codon. Here, we focused on roles A-rich region within burst sequence. We...
The genome of Bombyx mori nucleopolyhedrovirus (BmNPV) is predicted to contain six RING finger proteins: IAP1, ORF35, IAP2, CG30, IE2, and PE38. Several other members the family have recently been shown ubiquitin-ligase (E3) activity. We thus examined whether BmNPV proteins E3 In vitro ubiquitination assay with rabbit reticulocyte lysates fused maltose-binding protein (MBP) showed that four them (IAP2, PE38, CG30) were polyubiquitinated in presence zinc ion. Furthermore, MBP-IAP2, MBP-IE2,...
Wolbachia are intracellular bacteria in insects that can manipulate the sexual development and reproduction by male killing or other methods. We have recently identified a protein named Oscar acts as male‐killing factor for lepidopteran insects. interacts with Masculinizer (Masc) protein, which is required both masculinization dosage compensation (DC) Embryonic expression of inhibits causes two species, Ostrinia furnacalis Bombyx mori . However, it remains unknown whether Oscar‐induced...
The ubiquitin-proteasome system plays a central role in the degradation of intracellular proteins and is often required for efficient virus infection. Homologues ubiquitin are found all group I nucleopolyhedroviruses (NPVs), but their roles NPV infection still unclear. This study that specific proteasome inhibitor MG-132 markedly reduced budded (BV) production polyhedrin expression Bombyx mori (BmNPV)-infected BmN-4 cells. Western blot analysis revealed treatment cells with resulted delayed...
ABSTRACT Lepidopteran nucleopolyhedroviruses (NPVs) show distinct tissue tropism in host insect larvae. However, the molecular mechanism of this is largely unknown. We quantitatively investigated NPV by measuring mRNA levels viral genes 16 tissues from Bombyx mori (BmNPV)-infected B. larvae and found clear tropism, i.e., BmNPV replicates poorly silk glands, midgut, Malpighian tubule compared with other larval tissues. next identified determining infection investigating phenotypes infected 44...
In quantitative gene expression analysis, normalization using a reference as an internal control is frequently performed for appropriate interpretation of the results. Efforts have been devoted to exploring superior novel genes microarray transcriptomic data and evaluating commonly used by targeting analysis. However, because number specifically detectable totally dependent on probe design in exploration may miss some best choices genes. Recently emerging RNA sequencing (RNA-seq) provides...
Baculovirus IE2 functions as a transregulator and is also involved in viral DNA replication. However, the mechanism for these remains unknown. It has previously been reported that Bombyx mori nucleopolyhedrovirus (BmNPV) ubiquitin ligase activity dependent on RING finger domain can oligomerize through its C-terminal coiled-coil region. Here, confocal microscopy analysis demonstrated formed nuclear foci only during early phase of infection (2-6 h post-infection). Therefore, it was determined...
The Bombyx mori nucleopolyhedrovirus (BmNPV) ORF8 protein has previously been reported to colocalize with IE1 specific nuclear sites during infection. Transient expression of green fluorescent (GFP)-fused showed the have cytoplasmic localization, but following BmNPV infection formed foci, suggesting that requires some other viral factor(s) for this. Therefore, interacting factors were looked using yeast two-hybrid system and was identified. We mapped region a assay. An N-terminal (residues...
During the late stage of infection, alphabaculoviruses produce many occlusion bodies (OBs) in nuclei insect host's cells through hyperexpression polyhedrin (POLH), a major OB component encoded by polh. The strong polh promoter has been used to develop baculovirus expression vector system for recombinant protein cultured and larvae. However, relationship between POLH accumulation coding sequence remains largely unelucidated. This study aimed assess importance codon usage and/or nucleotide...
Abstract Parasitoid wasps, one of the most diverse and species-rich animal taxa on Earth, produce venoms that manipulate host development physiology to exploit resources. However, mechanisms venom action remain poorly understood. Here, we show infection Drosophila by endoparasitoid wasp, Asobara japonica , triggers imaginal disc degradation (IDD) inducing apoptosis, autophagy, mitotic arrest, leading impaired metamorphosis. A multi-omics approach identified two proteins A. necessary for IDD....
Abstract Wolbachia are the maternally transmitted bacterial symbionts that ubiquitous among arthropods. They can hijack host reproduction in various ways, including male killing (MK), where sons of infected mothers killed during development. The recent discovery MK-associated genes, i.e., oscar Ostrinia moths and wmk Drosophila flies, stimulates our interest diversity commonality MK mechanisms, which remain largely unclear. We recently discovered a symbiont moth Homona magnanima carries an...
Wolbachia are the maternally transmitted bacterial symbionts that ubiquitous among arthropods. They can hijack host reproduction in various ways, including male killing (MK), where sons of infected mothers killed during development. The recent discovery MK-associated genes, i.e., oscar Ostrinia moths and wmk Drosophila flies, stimulates our interest diversity commonality MK mechanisms, which remain largely unclear. We recently discovered a symbiont moth Homona magnanima carries an prophage...