Abstract Hydrogels are the focus of extensive research due to their potential use in fields including biomedical, pharmaceutical, biosensors, and cosmetics. However, general weak mechanical properties hydrogels limit utility. Here, pristine silk fibroin (SF) with excellent generated via a binary‐solvent‐induced conformation transition (BSICT) strategy. In this method, conformational SF is regulated by moderate binary solvent diffusion SF/solvent interactions. β‐sheet formation serves as...

In this study, we determined whether repeated brief isoflurane (Iso) anesthesia induces ischemic tolerance to focal cerebral ischemia in a dose-response manner and the effect is dependent on adenosine triphosphate-regulated potassium channels. Experiment 1, 40 rats were randomly assigned 4 groups: control animals received 100% oxygen 1 h/d for 5 days, whereas (Iso)1, Iso2, Iso3 groups 0.75%, 1.5%, or 2.25% Iso days. 2, 36 controls days; I+G (Iso+glibenclamide) 2% group glibenclamide (GLB) (5...

The present study examined the hypothesis that spinal cord ischemic tolerance induced by hyperbaric oxygen (HBO) preconditioning is triggered an initial oxidative stress and associated with increase of antioxidant enzyme activities as one effector neuroprotection. New Zealand White rabbits were subjected to HBO preconditioning, air (HBA) or sham pretreatment once daily for five consecutive days before ischemia. Activities catalase (CAT) superoxide dismutase increased in tissue group 24 h...

In this study, we determined whether repeated brief isoflurane (Iso) anesthesia induces ischemic tolerance to focal cerebral ischemia in a dose-response manner and the effect is dependent on adenosine triphosphate-regulated potassium channels. Experiment 1, 40 rats were randomly assigned 4 groups: control animals received 100% oxygen 1 h/d for 5 days, whereas (Iso)1, Iso2, Iso3 groups 0.75%, 1.5%, or 2.25% Iso days. 2, 36 controls days; I+G (Iso+glibenclamide) 2% group glibenclamide (GLB) (5...

Background The aim of this study was to determine if the ischemic tolerance could be induced in spinal cord by pretreatment with hyperbaric oxygen (HBO) and what components HBO (hyperoxia, hyperbaricity, combination these two) were critical induction against injury. Methods In experiment 1, 21 rabbits randomly assigned one three groups (n = 7 each): animals control group received no before ischemia; HBO-1 HBO-2 (2.5 atmosphere absolute [ATA], 100% O2) 1 h/day for 3 5 days ischemia,...

Silk of Bombyx mori can be used as various biomaterials. Especially, it is useful a protein for coating the surface cell culture plates since silk possesses biocompatibility to cultured cells. However, cell-adhesive ability weaker than collagen or fibronectin, which are plate more frequently (Yao et al. J. Biochem., 2004, 136, 643−649). To increase silk, we constructed transgenic silkworms, inserting modified fibroin light-chain genes making recombinant silks that possessed partial...

General anesthesia has been widely applied in surgical or nonsurgical medical procedures, but the mechanism behind remains elusive. Because of shared neural circuits sleep and anesthesia, whether serotonergic system, which is highly implicated modulation wakefulness, regulates general as well worth investigating.Immunostaining fiber photometry were used to assess neuronal activities. Electroencephalography spectra burst-suppression ratio (BSR) measure anesthetic depth loss recovery righting...

Abstract Materials with structural coloration capable of multimode color manipulation are gaining growing significance for advanced encryption and high‐security anti‐counterfeiting applications. Among the most promising candidates naturally derived biomaterials, owing to their renewable, biocompatible, biodegradable features developing sustainable, bio‐interfaced photonic platforms. Nevertheless, strategies developed from biological materials usually exhibit limited optical operation modes,...

Cholinergic arousal system plays an important role in the maintenance of consciousness. The authors investigated whether intrabasalis injection orexin-A or orexin-B and electrically stimulated pedunculopontine tegmentum nuclei (PPTg: origin cholinergic ascending pathways) may alter acetylcholine efflux electroencephalographic activity somatosensory cortex relation to orexinergic isoflurane-anesthetized rats.Either (10, 30, 100 pmol) (n = 6 each) was injected into basal forebrain while...

It remains to be established whether spinal cord ischemic tolerance can induced by limb remote preconditioning (RIPC), and the mechanisms underlying neuroprotective effects of RIPC on need clarified.Spinal ischemia was studied in New Zealand White rabbits. In experiment 1, all rabbits were subjected 20-min aortic occlusion. Thirty minutes before ischemia, sham intervention or achieved bilateral femoral artery occlusion (10 min ischemia/10 reperfusion, two cycles). Dimethylthiourea (500...

Ethyl pyruvate (EP) has been reported to offer a protective effect against ischemic injury through its antiinflammatory action. The nuclear protein high-mobility group box 1 (HMGB1) can activate inflammatory pathways when released from cells. This study was designed investigate the neuroprotective of EP spinal cord and potential role HMGB1 in this process.EP administered at various time points before or after 20 min ischemia male New Zealand rabbits. All animals were sacrificed 72 h...

Regenerated silk fibroin fibers from the cocoons of silkworm, Bombyx mori, were prepared with hexafluoro solvents, 1,1,1,3,3,3-hexafluoro-2-propanol (HFIP) or hexafluoroacetone-trihydrate (HFA), as dope solvents and methanol coagulation solvent. The regenerated fiber HFIP solution showed slightly larger tensile strength when draw ratio is 1:3 than that native fiber, but HFA much lower fiber. This difference in between two comes long-range orientation crystalline region rather short-range...

Background Sepsis management in the Intensive Care Unit (ICU) presents a significant challenge within contemporary healthcare. The primary lies ensuring timely and appropriate utilization of antibiotics. Inappropriate antibiotic use sepsis can result multitude adverse outcomes. There has been insufficient focus on thoroughly understanding resolving issues related to improper application antibiotics treatment by physicians pharmacists. This gap research is concerning, considering its...

Regenerated Bombyx mori silk fibers were spun from hexafluoro-iso-propanol solution of fibroin sponge in methanol used as a coagulant solvent and then elongated water.The stress-strain curves the regenerated changed dramatically depending on draw ratio structure was studied by 13 C CP/MAS NMR X-ray diffraction methods.The patterns spectra two with different ratios (1× 3×) native fiber are all β-sheet although fraction random coil/distorted β-turn decreases order 1×, 3× gradually.On other...

Background: To assess the prevalence and associated risk of potentially inappropriate medications (PIMs) prescribing in community-dwelling elderly patients China to examine most frequently used PIMs. This will provide a reference for formulation medication manuals further standardize use patients. Methods: We conducted cross-sectional retrospective study from April 1, 2020 30, 2020. Data aged ≥65 years were collected Hengjie (N=2,294), Loujiang (N=3,972), Tongxing communities (N=1,969)...

BACKGROUND: Minimum alveolar concentration (MAC) is used as the standard measure of potency for volatile anesthetic agents. However, there a lack effective and quantitative indicator combined multiple coadministered inhalation intravenous anesthetics. We hypothesized that an equivalent anesthetics, normalized by MAC derived from response surface models fraction (abbreviated eMAC fraction), can reflect total METHOD: Fifty-three patients receiving general anesthesia were enrolled. A random...

Objective To evaluate the effects of peroxisome proliferator‐activated receptor (PPAR)–γ agonist on induction regulatory T cells (Tregs) in a murine model allergic rhinitis. Study Design Randomized controlled trial. Setting Animal study. Subjects and Methods BALB/c mice that received ovalbumin sensitization challenge served as group (n = 6). Two separate groups 6 intragastric administration with PPAR‐γ pioglitazone (30 mg/kg/d) or plus antagonist GW9662 (0.5 mg/d) before each challenge. The...

Abstract Actinidia chinensis Planch. root extract (acRoots) as one of Chinese traditional medications has been applied for antitumor therapy decades, although the exact mechanisms have not revealed. Our present study aimed to define inhibitory specificity and pattern acRoots in lung cancer cell lines by comparing 40 types lines, select acRoots‐associated inflammation target genes from transcriptional profiles acRoots‐sensitive less‐sensitive validate correlation with prognosis patients...