A5088870282- Neuroscience and Neuropharmacology Research
- Ion channel regulation and function
- Neurobiology and Insect Physiology Research
- Insect Utilization and Effects
- Vestibular and auditory disorders
- Insect and Arachnid Ecology and Behavior
- Neural dynamics and brain function
- Cellular transport and secretion
- Nicotinic Acetylcholine Receptors Study
- Invertebrate Immune Response Mechanisms
- Photoreceptor and optogenetics research
- Genetics and Neurodevelopmental Disorders
- Neurogenesis and neuroplasticity mechanisms
- Stress Responses and Cortisol
- Memory and Neural Mechanisms
- Retinal Development and Disorders
- Pain Mechanisms and Treatments
- Neuropeptides and Animal Physiology
- Adipose Tissue and Metabolism
- Nitric Oxide and Endothelin Effects
- Biochemical Analysis and Sensing Techniques
- Molecular Sensors and Ion Detection
- Ion Transport and Channel Regulation
- Amino Acid Enzymes and Metabolism
- Autoimmune Neurological Disorders and Treatments
National Institute for Physiological Sciences
2007-2021
The Graduate University for Advanced Studies, SOKENDAI
2009-2021
National Institutes of Natural Sciences
2006-2014
Mitsubishi Group (Japan)
1998-2004
Nagoya University
1995-2000
Institute of Life Sciences
1999
Kanazawa University
1998
University of North Carolina at Chapel Hill
1995
Autoimmune forms of encephalitis have been associated with autoantibodies against synaptic cell surface antigens such as NMDA- and AMPA-type glutamate receptors, GABA<sub>B</sub> receptor, LGI1. However, it remains unclear how many autoantigens are yet to be defined. Using immunoproteomics, we identified the GABA<sub>A</sub> receptor in human sera from two patients diagnosed who presented cognitive impairment multifocal brain MRI abnormalities. Both had antibodies directed extracellular...
Dystonia is characterized by excessive involuntary and prolonged simultaneous contractions of both agonist antagonist muscles. Although the basal ganglia have long been proposed as primary region, recent studies indicated that cerebellum also plays a key role in expression dystonia. One hereditary form dystonia, rapid-onset dystonia with parkinsonism (RDP), caused loss function mutations gene for Na pump α3 subunit (ATP1A3). Little information available on affected brain regions mechanism...
Norepinephrine (NE) has been shown to elicit long-term facilitation of GABAergic transmission rat cerebellar Purkinje cells (PCs) through beta-adrenergic receptor activation. To further examine the locus and adrenoceptor subtypes involved in NE-induced transmission, we recorded inhibitory postsynaptic currents (IPSCs) evoked by focal stimulation with paired-pulse (PP) stimuli from PCs slices whole cell recordings analyzed PP ratio IPSC amplitude. NE increased amplitude a decease variance...
Abstract A major subtype of glutamate receptors, AMPA receptors (AMPARs), are generally thought to mediate excitation at mammalian central synapses via the ionotropic action ligand‐gated channel opening. It has recently emerged, however, that synaptic activation AMPARs by released from climbing fibre input elicits not only postsynaptic but also presynaptic inhibition GABAergic transmission onto Purkinje cells in cerebellar cortex. Although is critical for information processing synapses,...
A simple form of presynaptic plasticity, paired-pulse facilitation (PPF), has been explained as a transient increase in the probability vesicular release. Using whole-cell patch-clamp technique to record synaptic activity rat cerebellar slices, we found different forms presynaptically originated short-term plasticity during glutamatergic excitatory neurotransmission from granule cells (GCs) molecular-layer interneurones (INs). Paired-pulse activation GC axons at short intervals (30-100 ms)...
The effect of starvation on carbohydrate metabolism in the last instar larvae silkworm Bombyx mori was examined. Trehalose concentration hemolymph increased slightly during first 6 h and decreased thereafter, whereas glucose rapidly immediately after diet deprivation. Starvation-induced hypertrehalosemia completely inhibited by neck ligation, suggesting that stimulates release a hypertrehalosemic factor(s) from head. percentage active glycogen phosphorylase fat body within 3 its content...
The climbing fiber (CF) neurotransmitter not only excites the postsynaptic Purkinje cell (PC) but also suppresses GABA release from inhibitory interneurons converging onto same PC depending on AMPA-type glutamate receptor (AMPAR) activation. Although CF-/AMPAR-mediated inhibition of provides a likely mechanism boosting CF input-derived excitation, how transmitter reaches target AMPARs to elicit this action remains unknown. Here, we report that diffused its sites directly targets GluR2/GluR3...
Abstract While high threshold voltage‐dependent Ca 2+ channels (VDCCs) of the N and P/Q families are crucial for evoked neurotransmitter release in mammalian CNS, it remains unclear to what extent L‐type (LTCCs), which have been mainly considered as acting at postsynaptic sites, participate control transmitter release. Here, we investigate possible role LTCCs regulating GABA by cerebellar molecular layer interneurons (MLIs) from rats. We found that BayK8644 (BayK) markedly increases mIPSC...
The concomitant release of multiple numbers synaptic vesicles [multivesicular (MVR)] in response to a single presynaptic action potential enhances the flexibility transmission. However, molecular mechanisms underlying MVR at CNS synapse remain unclear. Here, we show that Ca v 2.1 subtype (P/Q-type) voltage-gated calcium channel is specifically responsible for induction MVR. In rat cerebellar cortex, paired-pulse activation granule cell (GC) ascending fibers leads not only facilitation peak...
Abstract Neurotransmitters diffuse out of the synaptic cleft and act on adjacent synapses to exert concerted control strength within neural pathways that converge single target neurons. The excitatory transmitter released from climbing fibers (CFs), presumably glutamate, is shown inhibit γ‐aminobutyric acid (GABA) release at basket cell (BC)–Purkinje (PC) in rat cerebellar cortex through its extrasynaptic diffusion activation α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic (AMPA) receptors...
Synaptic vesicle exocytosis is triggered by Ca2+ influx through several subtypes of voltage-gated calcium channels in the presynaptic terminal. We previously reported that paired-pulse stimulation at brief intervals increases Cav 2.1 (P/Q-type) channel-mediated multivesicular release (MVR) glutamatergic synapses between granule cells (GCs) and molecular layer interneurons (MLIs) rat cerebellar slices. However, it has yet to be determined how 2 channel take part MVR single axon This study...
Abstract Calcium influx into presynaptic terminals through voltage‐gated Ca 2+ channels triggers univesicular or multivesicular release of neurotransmitters depending on the characteristics machinery. However, mechanisms underlying (MVR) and its regulation remain unclear. Previous studies showed that in rat cerebellum, cyclin‐dependent kinase inhibitor roscovitine profoundly increases excitatory postsynaptic current (EPSC) amplitudes at granule cell (GC)‐Purkinje (PC) synapses by enhancing...