A5014321570- Retinal Development and Disorders
- Primate Behavior and Ecology
- Animal Behavior and Reproduction
- Photoreceptor and optogenetics research
- Olfactory and Sensory Function Studies
- Biochemical Analysis and Sensing Techniques
- Zebrafish Biomedical Research Applications
- Neurobiology and Insect Physiology Research
- Visual perception and processing mechanisms
- Circadian rhythm and melatonin
- Neuroendocrine regulation and behavior
- melanin and skin pigmentation
- Animal Vocal Communication and Behavior
- Genetic diversity and population structure
- RNA and protein synthesis mechanisms
- Plant and animal studies
- Wildlife Ecology and Conservation
- Evolutionary Psychology and Human Behavior
- Photochromic and Fluorescence Chemistry
- Bat Biology and Ecology Studies
- Amphibian and Reptile Biology
- Forensic and Genetic Research
- Advanced Chemical Sensor Technologies
- Identification and Quantification in Food
- Peptidase Inhibition and Analysis
The University of Tokyo
2016-2025
Ube Frontier University
2010-2020
Bioscience (China)
2017
Tokyo University of Science
1992-2010
Kyoto University
1981-2005
National Center of Neurology and Psychiatry
2005
Syracuse University
1993-1999
Nisshin Steel (Japan)
1976
Nagoya Institute of Technology
1975
Gunma University
1959
Proper functioning of sensory systems requires the generation appropriate numbers and proportions neuronal subtypes that encode distinct information. Perception color relies on signals from multiple cone photoreceptor types. In cone-dominated retinas, each expresses a single opsin type with peak sensitivity to UV, long (L) (red), medium (M) (green), or short (S) (blue) wavelengths. The modes cell division generating types are unknown. We report here mechanism whereby zebrafish photoreceptors...
Abstract Zebrafish is becoming a powerful animal model for the study of vision but genomic organization and variation its visual opsins have not been fully characterized. We show here that zebrafish has two red (LWS-1 LWS-2), four green (RH2-1, RH2-2, RH2-3, RH2-4), single blue (SWS2) ultraviolet (SWS1) opsin genes in genome, among which LWS-2, RH2-3 are novel. SWS2, LWS-1, LWS-2 located tandem RH2-1, RH2-4 form another gene cluster. The peak absorption spectra (λmax) reconstituted...
Abstract Cone photoreceptors in fish are typically arranged into a precise, reiterated pattern known as “cone mosaic.” mosaic patterns can vary different species and response to changes habitat, yet their function the mechanisms of development remain speculative. Zebrafish ( Danio rerio ) have four cone subtypes precise rows adult retina. Here we describe larval zebrafish investigate previously unrecognized transition between patterns. positions were determined transgenic expressing green...
SUMMARY Zebrafish have two red, LWS-1 and LWS-2, four green, RH2-1, RH2-2, RH2-3 RH2-4, opsin genes encoding photopigments with distinct absorption spectra. Occurrence of subtypes by gene duplication is characteristic fish but little known whether the are expressed differently in retina, either spatially or temporally. Here we show situ hybridization dynamic expression patterns zebrafish retina. Expression red type opsins initiated shorter-wavelength subtype followed longer-wavelength LWS-1....
Animal eyes are morphologically diverse. Their assembly, however, always relies on the same basic principle, i.e., photoreceptors located in vicinity of dark shielding pigment. Cnidaria as likely sister group to Bilateria earliest branching phylum with a well developed visual system. Here, we show that camera-type cubozoan jellyfish, Tripedalia cystophora, use genetic building blocks typical vertebrate eyes, namely, ciliary phototransduction cascade and melanogenic pathway. Our findings...
Trichromatic primates have a 'red-green' chromatic channel in addition to luminance and 'blue-yellow' channels. It has been argued that the red-green evolved as an adaptation for detecting reddish or yellowish objects, such ripe fruits, against background of foliage. However, foraging advantages trichromatic remain unverified by behavioral observation their natural habitats. New World monkeys (platyrrhines) are excellent model this evaluation because highly polymorphic nature color vision...
The capacity for cone‐mediated color vision varies among nocturnal primates. Some species are colorblind, having lost the functionality of their short‐wavelength‐sensitive‐1 ( SWS1 ) opsin pigment gene. In other species, such as aye‐aye D aubentonia madagascariensis ), gene remains intact. Recent studies focused on aye‐ayes indicate that this has been maintained by natural selection and a peak sensitivity (λ max 406 nm, which is ∼20 nm closer to ultraviolet region spectrum than in most...
Abstract Bipolar cells convey luminance, spatial, and color information from photoreceptors to amacrine ganglion cells. We studied the photoreceptor connectivity of 321 bipolar in adult zebrafish retina. 1,1′‐Dioctadecyl‐3,3,3′,3′‐tetramethylindocarbocyanine perchlorate (DiI) was inserted into whole‐mounted transgenic retinas label The that connect these DiI‐labeled were identified by fluorescence or their positions relative fluorescent cones, as cones are arranged a highly ordered mosaic:...
Abstract New World monkeys exhibit prominent colour vision variation due to allelic polymorphism of the long‐to‐middle wavelength (L/M) opsin gene. The known spectral L/M opsins in primates is broadly determined by amino acid composition at three sites: 180, 277 and 285 (the ‘three‐sites’ rule). However, two alleles found black‐handed spider ( Ateles geoffroyi ) are exceptions, presumably novel mutations. separation photopigments 1.5 times greater than expected based on rule. Yet consequence...
Significance In group-living mammals, infants of females with strong social relationships sometimes exhibit higher survivorship than less females, a finding that holds true in our study population wild white-faced capuchin monkeys. However, as many new alpha male capuchins often kill young sired by other males. Our long-term research shows highly are at greater risk dying or disappearing during periods replacements females. These findings indicate males more likely to target the social, and...
Human presence in Southeast Asia dates back to at least 40,000 years ago, when the current islands formed a continental shelf called Sundaland. In Philippine Islands, Peninsular Malaysia, and Andaman there exist indigenous groups collectively Negritos whose ancestry can be traced "First Sundaland People." To understand relationship between these Negrito their demographic histories, we generated genome-wide single nucleotide polymorphism data compared them with existing from other...
Ecological flexibility, extended lifespans, and large brains have long intrigued evolutionary biologists, comparative genomics offers an efficient effective tool for generating new insights into the evolution of such traits. Studies capuchin monkeys are particularly well situated to shed light on selective pressures genetic underpinnings local adaptation diverse habitats, longevity, brain development. Distributed widely across Central South America, they inventive extractive foragers, known...
Using only 11-cis 3, 4-dehydroretinal as a chromophore in the pure-cone retina, American chameleon (Anolis carolinensis) detects wide range of color from ultraviolet (UV) to infrared. We previously characterized its visual opsin genes sws1Ac, sws2Ac, rh2Ac, and lwsAc encode SWS1Ac, SWS2Ac, RH1Ac, RH2Ac, LWSAc opsins, respectively, pineal gland-specific opin (PAc) gene. Here we present light absorption profiles pigments obtained by expressing these opsins reconstituting them with retinal...
Cone photoreceptor cells of fish retinae are arranged in a highly organized fashion. However, the molecular mechanisms underlying development and retinal pattern formation largely unknown. Here we established transgenic lines zebrafish carrying green fluorescent protein (GFP) cDNA with 5.5‐kb upstream region ultraviolet‐sensitive cone opsin gene ( SWS1 ). In fish, GFP expression proceeded same spatiotemporal as embryos. adult retina, was observed throughout short single (SSC) layer where is...
Color vision is an important characteristic of primates and, intriguingly, Neotropical monkeys are highly polymorphic for this trait. Recent field studies have challenged the conventional view that trichromatic color more adaptive than dichromatic vision. No study has investigated pattern genetic variation in long to middle wavelength–sensitive (L-M or red–green) opsin gene as compared with other genomic regions (neutral references) wild populations New World look signature natural...
A fundamental step in the evolution of visual system is gene duplication opsins and differentiation between duplicates absorption spectra expression pattern retina. However, our understanding mechanism far behind that spectral tuning opsins. Zebrafish (Danio rerio) have two red-sensitive cone opsin genes, LWS-1 LWS-2. These genes are arrayed a tail-to-head manner, this order, both expressed long member double cones (LDCs) Expression longer-wave sensitive occurs later development thus...
Intraspecific color vision variation is prevalent among nearly all diurnal monkeys in the neotropics and seemingly a textbook case of balancing selection acting to maintain genetic polymorphism. Clear foraging advantages with trichromatic over those dichromatic "red-green colorblind" have been observed captive studies; however, evidence advantage during close-range has surprisingly scarce field studies, perhaps as result small sample sizes strong impacts environmental or individual on...
The signaling molecule retinoic acid (RA) regulates rod and cone photoreceptor fate, differentiation, survival. Here we elucidate the role of RA in differential regulation tandemly-duplicated long wavelength-sensitive (LWS) opsin genes. Zebrafish embryos were treated with from 48 hours post-fertilization (hpf) to 75 hpf, RNA was isolated eyes for microarray analysis. ~170 genes showed significantly altered expression, including several transcription factors components cellular pathways. Of...