A5019654275- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 detection and testing
- Membrane Separation Technologies
- Coastal and Marine Dynamics
- Bacteriophages and microbial interactions
- Viral Infections and Immunology Research
- Fecal contamination and water quality
- Animal Virus Infections Studies
- Respiratory viral infections research
- Parasitic Infections and Diagnostics
- Biosensors and Analytical Detection
- Water Treatment and Disinfection
- Aquatic Ecosystems and Phytoplankton Dynamics
- Marine and coastal ecosystems
- Virus-based gene therapy research
- Pharmaceutical and Antibiotic Environmental Impacts
- Biocrusts and Microbial Ecology
- Earthquake and Tsunami Effects
- Electrospun Nanofibers in Biomedical Applications
- Membrane-based Ion Separation Techniques
- Legionella and Acanthamoeba research
- Listeria monocytogenes in Food Safety
- Microbial Community Ecology and Physiology
- Nanopore and Nanochannel Transport Studies
- Ocean Waves and Remote Sensing
The University of Tokyo
2016-2025
Penta-Ocean Construction (Japan)
2003-2024
Kyoto University
2023
Bunkyo University
2019
Mitsubishi Heavy Industries (Germany)
2018
IHI Corporation (United States)
2018
Vietnam National University, Hanoi
2018
University of Yamanashi
2010
National Institute of Infectious Diseases
2010
National Archives and Records Administration
2008
ABSTRACT We developed a new procedure for concentration of enteric viruses from water using negatively charged membrane. Rinsing the membrane with 0.5 mM H 2 SO 4 (pH 3.0) in order to elute cations prior viral elution 1 NaOH 10.5) promoted poliovirus recovery yields 33 95% when applied pure and 38 89% natural seawater Tokyo Bay, Japan, respectively. This method showed average spiked 62% ( n = 8) liter artificial seawater. higher (>61%) than that conventional positively (6%) is also free...
ABSTRACT Pepper mild mottle virus (PMMoV) is a plant that has been recently proposed as potential indicator of human fecal contamination environmental waters; however, information on its geographical occurrence in surface water still limited. We aimed to determine the seasonal and geographic PMMoV drinking sources all over Japan. Between July 2008 February 2011, 184 source samples were collected from 30 treatment plants (DWTPs); viruses 1 2 liters each sample concentrated by using an...
The current situation of COVID-19 measures makes it difficult to accurately assess the prevalence SARS-CoV-2 due a decrease in reporting rates, leading missed initial transmission events and subsequent outbreaks. There is growing recognition that wastewater virus data assist estimating potential infections, including asymptomatic unreported infections. Understanding hidden behind reported cases critical for decision-making when choosing appropriate social intervention measures. However,...
The occurrence of human enteric viruses in surface water the Tamagawa River, Japan, was surveyed for 1 year, from April 2003 to March 2004. Sixty-four samples were collected six sites along river, and 500 ml sample concentrated using cation-coated filter method, which developed our previous study. This method showed recovery yields 56% +/- 32% (n = 37) inoculated with polioviruses. More than one kind tested virus detected 43 (67%) 64 by TaqMan PCR. Noroviruses adenoviruses a high positive...
Photoreactivation of Escherichia coli after inactivation by a low-pressure (LP) UV lamp (254 nm), medium-pressure (MP) (220 to 580 or filtered (MPF) (300 nm) was investigated. An endonuclease sensitive site (ESS) assay used determine the number UV-induced pyrimidine dimers in genomic DNA E. coli, while conventional cultivation investigate colony-forming ability (CFA) coli. In photoreactivation experiments, more than 80% induced LP MPF irradiation were repaired, almost no repair observed MP...
To evaluate and compare the reductions of human viruses F-specific coliphages in a full-scale wastewater treatment plant based on quantitative PCR (qPCR) plate count assays.A total 24 water samples were collected from four locations at plant, relative abundance F-RNA phage genogroups determined by qPCR. Of 10 types tested, enteric adenoviruses most prevalent both influent effluent samples. different steps, activated sludge process was effective reducing microbial loads. Viruses phages showed...
The primary concentration and molecular process are critical to implement wastewater-based epidemiology for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, the previously developed methods were optimized nonenveloped viruses. Few studies evaluated if applicable efficient recovery of enveloped viruses from various types raw sewage. This study aims (1) compare whole Pseudomonas phage φ6, a surrogate viruses, among combinations [ultrafiltration (UF), electronegative...
Polyethylene glycol (PEG) precipitation is one of the conventional methods for virus concentration. This technique has been used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater. The procedures and seeded surrogate viruses were different among implementers; thus, reported whole process recovery efficiencies considerably varied studies. present study compared five PEG procedures, with operational parameters, RT-qPCR-based efficiency murine hepatitis...
ABSTRACT UV inactivation, photoreactivation, and dark repair of Escherichia coli Cryptosporidium parvum were investigated with the endonuclease sensitive site (ESS) assay, which can determine UV-induced pyrimidine dimers in genomic DNA microorganisms. In a 99.9% inactivation E. , high correlation was observed between dose irradiation number induced . The colony-forming ability also correlated highly DNA, indicating that ESS assay is comparable to method conventionally used measure ability....
ABSTRACT A virus concentration method using a cation-coated filter was developed for large-volume freshwater applications. Poliovirus type 1 (LSc 2ab Sabin strain) inoculated into 40 ml of MilliQ (ultrapure) water adsorbed effectively to negatively charged (Millipore HA, 0.45-μm pore size) coated with aluminum ions, 99% (range, 81 114%) which were recovered by elution 1.0 mM NaOH (pH 10.8) following an acid rinse 0.5 H 2 SO 4 3.0). More than 80% poliovirus recovery yields obtained from...
The seasonal profiles of microorganisms in raw sewage, secondary-treated and final effluent at a wastewater treatment plant Tokyo, Japan, were quantitatively determined each month for one year, from July 2003 to June 2004. Human noroviruses, which by real-time PCR, sewage varied 0.17–260 copies/mL genotype 1 2.4–1900 2, showing much higher values winter, the epidemic season. concentration total coliforms, Escherichia coli, or F-specific phages was almost constant throughout year. noroviruses...
Aims: The aim of this study was to determine human adenoviruses (HuAdVs) in aquatic environments by real‐time polymerase chain reaction (PCR). Methods and Results: In order describe the ratio enteric serotypes total HuAdVs, primer set specific for 40 41 used parallel with universal all 51 HuAdVs. HuAdVs were detected at concentration 7·3–1500 PCR‐detection units (PDU) per ml raw sewage (n = 17), 0·00060–4·1 PDU ml−1 secondary‐treated before chlorination 0·0018–7·0 river water 36), 0·032–6·1...
Inhibitors that reduce viral nucleic acid extraction efficiency and interfere with cDNA synthesis and/or polymerase activity affect the molecular detection of viruses in aquatic environments. To overcome these significant problems, we developed a methodology for assessing yields DNA amplification efficiencies environmental water samples. This involved adding particles adenovirus type 5 murine norovirus newly primer-sharing controls, which are amplified same primer pairs result amplicon sizes...
ABSTRACT Aichi viruses (AiVs) have been proposed as a causative agent of human gastroenteritis potentially transmitted by fecal-oral routes through contaminated food or water. In the present study, we developed TaqMan minor groove binder (MGB)-based reverse transcription-quantitative PCR (RT-qPCR) system that is able to quantify AiVs and differentiate between genotypes A B. This consists two assays, an AiV universal assay utilizing primer pair probe duplex genotype-specific same probes. The...
ABSTRACT To evaluate the effectiveness of UV irradiation in inactivating Cryptosporidium parvum oocysts, animal infectivities and excystation abilities oocysts that had been exposed to various doses were determined. Infectivity decreased exponentially as dose increased, required for a 2-log 10 reduction infectivity (99% inactivation) was approximately 1.0 mWs/cm 2 at 20°C. However, C. exhibited high resistance irradiation, requiring an extremely 230 excystation, which used assess viability....