A5007728357- SARS-CoV-2 detection and testing
- Viral gastroenteritis research and epidemiology
- Biosensors and Analytical Detection
- Fecal contamination and water quality
- SARS-CoV-2 and COVID-19 Research
- Child Nutrition and Water Access
- Water Treatment and Disinfection
- Viral Infections and Immunology Research
- Vibrio bacteria research studies
- Groundwater flow and contamination studies
- Advanced biosensing and bioanalysis techniques
- Animal Disease Management and Epidemiology
- Antibiotic Resistance in Bacteria
- Mycobacterium research and diagnosis
- Pharmaceutical and Antibiotic Environmental Impacts
- Molecular Biology Techniques and Applications
- Animal Virus Infections Studies
- Microbial Community Ecology and Physiology
- Antibiotic Use and Resistance
- Escherichia coli research studies
- Respiratory viral infections research
- Environmental Justice and Health Disparities
- Salmonella and Campylobacter epidemiology
- Cytomegalovirus and herpesvirus research
- Bacillus and Francisella bacterial research
University of Yamanashi
2017-2025
Takeda (Japan)
2017-2025
Wastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in community because severe acute respiratory syndrome 2 (SARS-CoV-2), etiological agent COVID-19, can be shed feces infected individuals regardless their symptoms. The present study aimed assess presence SARS-CoV-2 RNA wastewater and river water Yamanashi Prefecture, Japan, using four quantitative two nested PCR assays. Influent secondary-treated (before chlorination)...
Polyethylene glycol (PEG) precipitation is one of the conventional methods for virus concentration. This technique has been used to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in wastewater. The procedures and seeded surrogate viruses were different among implementers; thus, reported whole process recovery efficiencies considerably varied studies. present study compared five PEG procedures, with operational parameters, RT-qPCR-based efficiency murine hepatitis...
The applicability of wastewater-based epidemiology (WBE) has been extensively studied throughout the world with remarkable findings. This study reports presence and reduction severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) at two wastewater treatment plants (WWTPs) Nepal, along river water, hospital (HWW), from sewer lines collected between July 2020 February 2021. SARS-CoV-2 RNA was detected in 50%, 54%, 100%, 100% water samples WWTPs, hospitals, lines, respectively, by least...
Variants of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are continuously emerging, highlighting the importance regular surveillance SARS-CoV-2 and other epidemiologically significant pathogenic viruses in current context. Reverse transcription-quantitative PCR (RT-qPCR) is expensive, time-consuming, labor-intensive, requires a large reagent volume, only tests few targets single run. High-throughput qPCR (HT-qPCR) utilizing Biomark HD system (Fluidigm) can be used as an...
Dengue virus (DENV) is an enveloped, single-stranded RNA that causes approximately 390 million infections, leading to 40,000 deaths annually. Due the increasing trend of urbanization, water supply scarcity, and climate change, dengue regarded as “disease future,” requiring robust surveillance for early detection DENV infection. Since shed in urine saliva persists wastewater at different temperatures, our study conducted a novel approach monitor outbreaks Kathmandu Valley, Nepal. The viral...
The role of wastewater-based epidemiology (WBE), a powerful tool to complement clinical surveillance, has increased as many grassroots-level facilities, such municipalities and cities, are actively involved in wastewater monitoring, the testing coronavirus disease 2019 (COVID-19) is downscaled widely. This study aimed conduct long-term surveillance severe acute respiratory syndrome 2 (SARS-CoV-2) Yamanashi Prefecture, Japan, using one-step reverse transcription-quantitative polymerase chain...
ABSTRACT Wastewater-based epidemiology is a powerful tool to understand the actual incidence of coronavirus disease 2019 (COVID-19) in community because severe acute respiratory syndrome 2 (SARS-CoV-2), etiological agent COVID-19, can be shed feces infected individuals regardless their symptoms. The present study aimed assess presence SARS-CoV-2 RNA wastewater and river water Yamanashi Prefecture, Japan, using four quantitative two nested PCR assays. Influent secondary-treated (before...
The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants is a major public health concern that has highlighted the need to monitor circulating strains better understand disease 2019 (COVID-19) pandemic. This study was carried out SARS-CoV-2 RNA and its variant-specific mutations in wastewater using reverse transcription-quantitative polymerase chain reaction (RT-qPCR). One-step RT-qPCR Detection Kit for Wastewater (Takara Bio), which amplified two N-gene regions...
A rapid virus concentration method is needed to get high throughput. Reliable results of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) detection in wastewater are necessary for applications wastewater-based epidemiology. In this study, an automated filtration using a concentrating pipette (CP Select; Innovaprep) was applied detect SARS-CoV-2 samples with several modifications increase its sensitivity and The performance the CP Select compared other methods (polyethylene glycol...
Comprehensive data on bacterial and viral pathogens of diarrhea studies applying culture-independent methods for examining antibiotic resistance in wastewater are lacking. This study aimed to simultaneously quantify genes (ARGs), class 1 integron-integrase (int1), diarrhea, 16S rRNA, other indicators using a high-throughput quantitative PCR (HT-qPCR) system. Thirty-six grab samples from treatment plant Japan three times month between August 2022 July 2023 were centrifuged, followed by...
Wastewater-based epidemiology (WBE) is a critical tool for monitoring community health. Although much attention has focused on severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), causative agent of disease 2019 (COVID-19), other pathogens also pose significant health risks. This study quantified the presence SARS-CoV-2, influenza A virus (Inf-A), and noroviruses genogroups I (NoV-GI) II (NoV-GII) in wastewater samples collected weekly (n = 170) from July 2023 to February 2024 five...
Quantification of waterborne pathogens in water sources is essential for alerting the community about health hazards. This study determined presence human enteric viruses and protozoa Bagmati River, Nepal, detected fecal indicator bacteria (total coliforms, Escherichia coli, Enterococcus spp.), human-fecal markers (human Bacteroidales JC BK polyomaviruses), index (tobacco mosaic virus pepper mild mottle virus). During a one-year period between October 2015 September 2016, total 18 surface...
To validate host-specific Bacteroidales assays to identify faecal-source contamination of drinking water sources in the Kathmandu Valley, Nepal.A total 54 composite samples were collected from human sewage, ruminants, pigs, dogs, chickens and ducks, which analysed by quantitative polymerase chain reaction using human-specific (BacHum, HF183 SYBR, gyrB TaqMan), ruminant-specific (BacCow BacR), pig-specific (Pig2Bac PF163) dog-specific (BacCan SYBR). The BacHum, BacR Pig2Bac judged best...
This study identified the sources of fecal contamination in groundwater different land covers. A total 300 samples were collected Kathmandu Valley, Nepal, dry (n = 152) and wet 148) seasons 2016. Fecal indicator bacteria initially enumerated, then using human (BacHum), ruminant (BacR), pig-associated (Pig2Bac) Bacteroidales quantitative polymerase chain reaction assays. Sixty-six percent (197/300) tested had Escherichia coli concentrations higher than World Health Organization threshold for...
Hand, foot, and mouth disease (HFMD) is a highly contagious that primarily affects children under five years of age. It mainly caused by serotypes Enterovirus A (EVA): EVA71, Coxsackievirus types 6 (CVA6), 10 (CVA10), 16 (CVA16). Despite being prevalent in Japan other countries the Asia-Pacific region, few studies have investigated HFMD pathogens wastewater. The present study aimed to develop sensitive broadly reactive quantitative polymerase chain reaction (qPCR) assay dominant serotype...