A5041431555- Glycosylation and Glycoproteins Research
- Lipid Membrane Structure and Behavior
- RNA and protein synthesis mechanisms
- RNA Interference and Gene Delivery
- Carbohydrate Chemistry and Synthesis
- Immunotherapy and Immune Responses
- Advanced biosensing and bioanalysis techniques
- Protein Structure and Dynamics
- Pancreatitis Pathology and Treatment
- Galectins and Cancer Biology
- Molecular spectroscopy and chirality
- Chemical Synthesis and Analysis
- DNA and Nucleic Acid Chemistry
- Trypanosoma species research and implications
- Immune Response and Inflammation
- Proteoglycans and glycosaminoglycans research
- Research on Leishmaniasis Studies
- Antimicrobial Peptides and Activities
- Bacterial Genetics and Biotechnology
- Muscle Physiology and Disorders
- Endoplasmic Reticulum Stress and Disease
- Biochemical effects in animals
- Advanced NMR Techniques and Applications
- Nicotinic Acetylcholine Receptors Study
- Pancreatic and Hepatic Oncology Research
University of Debrecen
2008-2024
Hungarian Academy of Sciences
2020-2022
Heidelberg Institute for Theoretical Studies
2019-2020
Széchenyi István University
2020
Ghent University
2013-2020
University of Pecs
2020
Max Planck Institute for Medical Research
2006-2010
European Molecular Biology Organization
2008-2010
European Molecular Biology Laboratory
2008
Max Planck Society
2008
N-Glycosylation starts in the endoplasmic reticulum (ER) where a 14-sugar glycan composed of three glucoses, nine mannoses, and two N-acetylglucosamines (Glc(3)Man(9)GlcNAc(2)) is transferred to nascent proteins. The glucoses are sequentially trimmed by ER-resident glucosidases. Glc(3)Man(9)GlcNAc(2) moiety substrate for oligosaccharyltransferase; Glc(1)Man(9)GlcNAc(2) Man(9)GlcNAc(2) intermediates signals glycoprotein folding quality control calnexin/calreticulin cycle. Here, we report...
Hypertriglyceridemia is the third most common cause of acute pancreatitis (AP). It has been shown that hypertriglyceridemia aggravates severity and related complications AP; however, detailed analyses large cohorts are contradictory. Our aim was to investigate dose-dependent effect on AP. AP patients over 18 years old who underwent triglyceride measurement within initial three days were included into our cohort analysis from a prospective international, multicenter registry operated by...
Quantum dot core–shell structures are essential to superior optical properties. However, Cd-based quantum dots grown from wurtzite CdSe cores still suffer irreproducibility due gel formation during the core synthesis. Moreover, state-of-the-art shelling procedures often remain time-consuming. We report here (1) a new procedure of synthesis which eliminates phosphonic anhydride contamination and (2) improvement generalization flash for growth highly luminescent multishell heterostructures....
The endoplasmic reticulum malectin is a highly conserved protein in the animal kingdom that has no counterpart so far lower organisms. We recently determined structure of its domain and found selective binding to Glc(2)Man(9)GlcNAc(2), an intermediate N-glycosylation. In our quest for putative ligands during initial characterization protein, we noticed specific diglucosides but quite tolerant towards linkage glucosidic bond. To understand molecular requirements observed promiscuity domain,...
Steroid-resistant nephrotic syndrome (SRNS) is a frequent cause of chronic kidney disease. Here, we identified recessive mutations in the gene encoding actin-binding protein advillin (AVIL) 3 unrelated families with SRNS. While all AVIL resulted marked loss its actin-bundling ability, truncation also disrupted colocalization F-actin, thereby leading to impaired actin binding and severing. Additionally, colocalized interacted phospholipase enzyme PLCE1 ARP2/3 actin-modulating complex....
Background C-reactive protein level (CRP) and white blood cell count (WBC) have been variably used in clinical trials on acute pancreatitis (AP). We assessed their potential role. Methods First, we investigated studies which CRP or WBC, to describe current role AP. Second, extracted the data of 1435 episodes AP from our registry. WBC admission, within 24 hours onset pain highest values were analyzed. Descriptive statistical tools as Kruskal-Wallis, Mann-Whitney U, Levene's F tests, Receiver...
Abstract During muscle cell differentiation, the alternatively spliced, acidic β-domain potentiates transcription of Myocyte-specific Enhancer Factor 2 (Mef2D). Sequence analysis by FuzDrop method indicates that can serve as an interaction element for Mef2D higher-order assembly. In accord, we observed mobile nuclear condensates in C2C12 cells, similar to those formed through liquid-liquid phase separation. addition, found solid-like aggregates cytosol, presence which correlated with higher...
Human Galectin-3 (
We report an efficient and adaptable method for the microencapsulation of active ingredients by a polydimethylsiloxane (PDMS) shell material. The core–shell microcapsules were obtained phase separation between core component PDMS components after repartitioning common solvent THF PDMS/core material water phase. For components, two commercially available functional polymers containing thiol vinyl side groups used. Photo-cross-linking in presence 2,2-dimethoxy-2-phenylacetophenone (DMPA)...
Abstract Biocompatible inorganic nano‐ and microcarriers can be suitable candidates for protein delivery. This study demonstrates facile methods of functionalization by using nanoscale linker molecules to change the adsorption capacity hydroxyapatite (HA) powder. The bovine serum albumin as a model has been studied with respect surface modifications. selected (lysine, arginine, phosphoserine) influence changing electrostatic nature HA surface. Qualitative quantitative analyses...
With the aim to develop compounds able target multiple metabolic pathways and, thus, lower chances of drug resistance, we investigated anti-trypanosomal activity and selectivity a series symmetric diglycosyl diselenides disulfides. Of 18 tested fully acetylated forms di-β-D-glucopyranosyl di-β-D-galactopyranosyl (13 15, respectively) displayed strong growth inhibition against bloodstream stage African trypanosomes (EC50 0.54 μM for 13 1.49 15) although with rather low (SI < 10 assayed murine...
Computational description of conformational and dynamic properties anticoagulant heparin analogue pentasaccharides is crucial importance in understanding their biological activities. We designed synthesized idraparinux derivatives modified with sulfonatomethyl moieties at the D, F, H glucose units that display varied potencies depending on exact nature substitution. In this report we examined capability molecular dynamics (MD) simulations to describe behavior these novel derivatives. used...
Abstract The viscosin group covers a series of cyclic lipodepsipeptides (CLPs) produced by Pseudomonas bacteria, with range biological functions and antimicrobial activities. Their oligopeptide moieties are composed both L ‐ D ‐amino acids. Remarkably, the Leu5 amino acid—centrally located in nonapeptide sequence—is sole residue found to possess either an or configuration, depending on producing strain. impact this / switch solution conformation was investigated NMR‐restrained molecular...
Abstract The voltage-gated Kv1.3 K + channel plays a key role in the activation of T lymphocytes. blockers selectively suppress immune responses mediated by effector memory cells, which indicates great potential selective inhibitors therapy certain autoimmune diseases. Anuroctoxin (AnTx), 35-amino-acid scorpion toxin is high affinity blocker Kv1.3, but also blocks Kv1.2 with similar potency. We designed and produced three AnTx variants: ([F32T]-AnTx, [N17A]-AnTx, [N17A/F32T]-AnTx) using...
Trypanosoma brucei, the causative agent of African sleeping sickness, encodes three cysteine homologues (Px I-III) classical selenocysteine-containing glutathione peroxidases. The enzymes obtain their reducing equivalents from unique trypanothione (bis(glutathionyl)spermidine)/tryparedoxin system. During catalysis, these tryparedoxin peroxidases cycle between an oxidized form with intramolecular disulfide bond Cys(47) and Cys(95) reduced peroxidase both residues in thiol state. Here we...
Competition binding experiments are used in NMR-based screenings to match up the site with that of a known ligand and determine strength interaction, thus providing ranking hits according receptor affinity. These competition titration must use reference for which on affinity interaction is known. rely observation separate signals hit compounds, very often hampered by signal overlap. Here, we present combination isotope editing filtering schemes saturation transfer difference (STD) allows...
G protein-coupled receptors (GPCRs) play an important role in many cellular responses; as such, their mechanism of action is utmost interest. To gain insight into the active conformation GPCRs, X-ray crystal structures nanobody (Nb)-stabilized β2 -adrenergic receptor (β2 AR) have been reported. Nb80, particular, able to bind intracellular protein binding site AR and stabilize conformation. Within complementarity-determining region 3 (CDR3) responsible for most interactions. Hence, we...
The predictable 3D structure of double-stranded DNA renders it ideally suited as a template for the bottom-up design functionalized nucleic acid-based active sites. We here explore use 14mer duplex scaffold precise and positioning catalytic functionalities. Given ubiquitous participation histidine-based imidazole group in protein recognition catalysis events, single histidine-like modified duplexes were investigated. Tethering histamine to C5 thymine base via an amide bond, allows flexible...