A5077611260- Bioactive Compounds and Antitumor Agents
- Pain Mechanisms and Treatments
- Cancer therapeutics and mechanisms
- Synthesis and Biological Evaluation
- Cancer-related Molecular Pathways
- Cannabis and Cannabinoid Research
- Botulinum Toxin and Related Neurological Disorders
- Neuroscience of respiration and sleep
- Barrier Structure and Function Studies
- Synthesis of Organic Compounds
- Connective Tissue Growth Factor Research
- Cell death mechanisms and regulation
- Synthesis and biological activity
- Cancer Mechanisms and Therapy
- Hepatocellular Carcinoma Treatment and Prognosis
- Hereditary Neurological Disorders
- Wnt/β-catenin signaling in development and cancer
- Spinal Cord Injury Research
- Dialysis and Renal Disease Management
- Fibroblast Growth Factor Research
- Phagocytosis and Immune Regulation
- Liver physiology and pathology
- Cancer, Lipids, and Metabolism
- Parathyroid Disorders and Treatments
- Cancer-related molecular mechanisms research
Mackay Medical College
2012-2022
National Chung Hsing University
2017
National Taipei University
2017
National Taiwan University
1991-2017
Taipei Veterans General Hospital
2001-2017
China Medical University
2015
Taipei Medical University
2015
National Yang Ming Chiao Tung University
1999-2014
Institute of Cell Biology and Neurobiology
2013
Mackay Memorial Hospital
2013
MicroRNAs (miRNAs), which are inhibitors of gene expression, participate in diverse biological functions and carcinogenesis. In this study, we show that liver-specific microRNA-122 (miR-122) is significantly down-regulated liver cancers with intrahepatic metastasis negatively regulates tumorigenesis. Restoration miR-122 metastatic Mahlavu SK-HEP-1 cells reduced vitro migration, invasion, anchorage-independent growth as well vivo tumorigenesis, angiogenesis, an orthotopic cancer model....
The aggressiveness of a tumor is partly attributed to its resistance chemotherapeutic agent-induced apoptosis. Cysteine-rich 61 (Cyr61), from the CCN gene family, secreted and matrix-associated protein, which involved in many cellular activities such as growth differentiation. Here we established cell model system examine whether stable expression Cyr61 MCF-7 cells can confer apoptosis identify possible participating mechanisms. We showed that lines overexpressing had acquired remarkable...
Tumor invasion and metastasis cause most deaths in cancer patients. Connective tissue growth factor (CTGF), a secreted protein that binds to integrins, modulates the invasive behavior of certain human cells, but few mechanistic details are known. We investigated roles CTGF collapsin response mediator 1 (CRMP-1) lung adenocarcinoma.We compared vector control-transfected cells with corresponding gene-transfected cells. Invasive activity was measured modified Boyden chamber assay, metastatic an...
Ten-eleven translocation methylcytosine dioxygenase 1 (Tet1) mediates the conversion of 5-methylcytosine (5 mC) to 5-hydroxymethylcytosine hmC), hence promoting DNA demethylation. Although recent studies have linked demethylation specific genes pain hypersensitivity, role spinal Tet1-dependent in nociception hypersensitivity development remains elusive. Here, we report correlated with behavioral allodynia, nerve ligation (SNL) upregulated Tet1 expression dorsal horn neurons that hydroxylate...
Abstract Background: The histone deacetylases (HDACs) have been implicated in pain hypersensitivity. This study investigated the potential involvement of an HDAC4-related mechanism spinal nerve ligation (SNL)-induced nociceptive Methods: left L5 to L6 nerves 627 adult male Sprague–Dawley rats were surgically ligated. withdrawal threshold hind paws and abundances, cellular location, interactions proteins dorsal horn assayed before after surgery. 14-3-3β-targeting small-interfering RNA, a...
The process of vascular calcification has been associated with the canonical Wnt/β-catenin signalling pathway in cell cultures and animal studies. relationship between circulating inhibitors dialysis patients is unknown. aim this study was to investigate associations serum dickkopf-1 (Dkk-1) sclerostin, two pathway, severity aortic (AoC) cardiovascular outcomes patients. This a prospective observational cohort study. One hundred twenty-five on maintenance haemodialysis participated Serum...
Melatonin (MLT; N-acetyl-5-methoxytryptamine) exhibits analgesic properties in chronic pain conditions. While researches linking MLT to epigenetic mechanisms have grown exponentially over recent years, very few studies investigated the contribution of MLT-associated modification states. Here, we report that together with behavioral allodynia, spinal nerve ligation (SNL) induced a decrease expression catalytic subunit phosphatase 2A (PP2Ac) and enhanced histone deacetylase 4 (HDAC4)...
Impaired wound healing is a serious problem for diabetic patients. Wound complex process that requires the cooperation of many cell types, including keratinocytes, fibroblasts, endothelial cells, and macrophages. β-Lapachone, natural compound extracted from bark lapacho tree ( Tabebuia avellanedae), well known its antitumor, antiinflammatory, antineoplastic effects at different concentrations conditions, but on have not been studied. The purpose present study was to investigate β-lapachone...
Emerging evidence has indicated that the pathogenesis of neuropathic pain is mediated by spinal neural plasticity in dorsal horn, which provides insight for analgesic therapy. Here, we report abundance tumor necrosis factor receptor-associated 2 and NcK-interacting kinase (TNIK), a presumed to regulate plasticity, was specifically enhanced ipsilateral horn neurons after nerve ligation (SNL; left L5 L6). Spinal TNIK-associated allodynia downstream TNIK–GluR1 coupling subsequent...
Retromer, which crucially contributes to endosomal sorting machinery through the retrieval and recycling of signaling receptors away from degradation, has been identified as a critical element for glutamatergic-receptor-dependent neural plasticity at excitatory synapses. We observed it accompanied by behavioral allodynia; neuropathic injury time-dependently enhanced VPS26A SNX27 expression; VPS26A–SNX27 coprecipitation; VPS26A-positive, SNX27-positive, double-labeled immunoreactivity in...
Spinal plasticity, a key process mediating neuropathic pain development, requires ubiquitination-dependent protein turnover. Presynaptic active zone proteins have crucial role in regulating vesicle exocytosis, which is essential for synaptic plasticity. Nevertheless, the mechanism ubiquitination-regulated turnover of presynaptic progression spinal plasticity-associated remains unclear. Here, after research involving Sprague Dawley rats, we reported that nerve ligation (SNL), addition to...
The areca nut-chewing habit is common in Southeast Asia, India, and Taiwan, arecoline the most abundant potent component nut. effects of on birth defects have been explored many species, including chicken, mice, zebrafish. embryos after long-term exposure are well established; however, short-term embryo to not understood. Using zebrafish, we study model human during early pregnancy. Arecoline, at concentrations from 0.001% 0.04%, was administered zebrafish 4 24 hours post fertilization....
Neuroligin-1 (NL1) forms a complex with the presynaptic neurexin-1β (Nrx1b), regulating clustering of N-methyl-D-aspartate receptors postsynaptic density-95 (PSD-95) to underlie learning-/memory-associated plasticity. Pain-related spinal neuroplasticity shares several common features The authors thereby investigated potential involvement NL1-related mechanism in nerve ligation (SNL)-associated allodynia.In 626 adult male Sprague-Dawley rats, withdrawal threshold and NL1, PSD-95,...
β-Lapachone, a novel anti-neoplastic drug, induces various cancer cells to undergo apoptosis. In previous report, we showed that β-lapachone-induced apoptosis of HL-60 is mediated by oxidative stress. However, in the present study, found human prostate (HPC) may be independent contrast 10-fold increase H<sub>2</sub>O<sub>2</sub>production seen cells, only 2- 4-fold was observed HPC cells. <i>N</i>-acetyl-l-cysteine (NAC), thiol antioxidant, inhibited DU145 after 12 h exposure β-lapachone....
Bromodomain-containing protein 4 binds acetylated promoter histones and promotes transcription; however, the role of bromodomain-containing in inflammatory hyperalgesia remains unclear.Male Sprague-Dawley rats received hind paw injections complete Freund's adjuvant to induce hyperalgesia. The dorsal root ganglia were examined detect changes expression activation genes involved voltage-gated sodium channel 1.7, which is a key pain-related ion channel.The intraplantar resulted thermal (4.0 ±...
Growth arrest and DNA-damage-inducible protein 45β reactivates methylation-silenced neural plasticity-associated genes through DNA demethylation. However, growth 45β-dependent demethylation contributes to neuropathic allodynia-associated spinal plasticity remains unclear.Adult male Sprague-Dawley rats (654 out of 659) received a nerve ligation or sham operation with without intrathecal application one the following: messenger RNA-targeted small interfering RNA, lentiviral vector expressing...