A5037249264- Immunotherapy and Immune Responses
- SARS-CoV-2 and COVID-19 Research
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- RNA Interference and Gene Delivery
- Influenza Virus Research Studies
- vaccines and immunoinformatics approaches
- Cancer Immunotherapy and Biomarkers
- Viral Infections and Outbreaks Research
- CAR-T cell therapy research
- Mosquito-borne diseases and control
- Animal Virus Infections Studies
- T-cell and B-cell Immunology
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Vectors
- Viral gastroenteritis research and epidemiology
- interferon and immune responses
- Respiratory viral infections research
- Virus-based gene therapy research
- Lung Cancer Research Studies
- Viral Infections and Immunology Research
- Immune cells in cancer
- Bacterial Infections and Vaccines
- Animal Disease Management and Epidemiology
- Pneumonia and Respiratory Infections
National Health Research Institutes
2016-2025
China Medical University
2015-2024
Kaohsiung Medical University
2018-2024
National Defense Medical Center
1998-2021
National Institute of Infectious Diseases
2012-2015
China Medical University
2015
Institute of Immunology
2012-2014
National Institutes of Health
1998-2014
Institute of Life Sciences
2014
Institut de Recherche Vaccinale
2012
A phase 1 study was conducted to assess the safety and immunologic response induced by direct injection of autologous immature dendritic cells (DCs) into tumor under radiotherapy in advanced hepatoma patients. Patients with advanced/metastatic stage not suitable for surgery or transarterial embolization were enrolled. Groups patients received two vaccinations. Each vaccination consisted intratumoral injections DCs four dose cohorts 5 x 10(6), 1.5 10(7), 3 10(7) 2 days after a single fraction...
Mesenchymal stem/stromal cells (MSCs) are promising potential candidates for the treatment of immunological diseases because their immunosuppressive functions. However, molecular mechanisms that mediate MSCs' activity remain elusive. In this article, we report first time, to our knowledge, secreted growth-regulated oncogene (GRO) chemokines, specifically GRO-γ, in human MSC-conditioned media have an effect on differentiation and function monocyte-derived dendritic cells. The were driven...
Abstract Cross-talk between TGF-β and IL-6 has been shown to direct the differentiation of CD4+ cells into special IL-17-secreting cells, which are termed Th17 cells. In this study, we demonstrated that could stimulate CD8+ differentiate noncytotoxic, IL-17-producing in MLC. These exhibit a unique granzyme B−IFN-γ−IL-10− phenotype. The mRNA level Th2/T cytotoxic 2 (Tc2) transcription factors GATA3 Th1/Tc1 T-box expressed T cell (T-bet) as well its target H2·O-like homeobox (Hlx) is decreased...
Most therapeutic mAbs target the receptor-binding domain (RBD) of spike protein SARS-CoV-2. Unfortunately, RBD is a hot spot for mutations in SARS-CoV-2 variants, which will lead to loss neutralizing function current mAbs. Universal different variants are necessary. We identified that recognized S2 region protein, identical variants. The could neutralize infection and protect animals from challenge. After cloning variable light chain heavy chain, sequences were humanized select high-affinity...
A major challenge in the use of DNA vaccines is efficient delivery vivo. Establishing a safe and electric transfer method key to developing rapid against emerging infectious diseases. To overcome complexity designing new machines for delivery, clinically approved machine could be considered as an alternative. Here, we report electroacupuncture machine-based vaccine after intramuscular injection COVID-19 vaccine. The S gene SARS-CoV-2 pVAX1 plasmid (pSARS2-S) was used antigen this study. We...
Abstract Cross-presentation is a key function of dendritic cells (DCs), which present exogenous Ags on MHC class I molecules to prime CTL responses. The effects TLR triggering the cross-presentation by DCs remain unclear. In this study, we used synthetic dipalmitoylated peptides and TLR2 agonist–conjugated as models elucidate mechanisms TLR2-mediated cross-presentation. We observed that internalization bone marrow–derived was facilitated via clathrin-mediated endocytosis. administration...
Although cytotoxic T lymphocytes (CTLs) play a major role in eradicating cancer cells during immunotherapy, the cancer-associated immunosuppressive microenvironment often limits success of such therapies. Therefore, simultaneous induction cancer-specific CTLs and reversal tumor may be more effectively achieved through single therapeutic vaccine. A recombinant lipoprotein with intrinsic Toll-like receptor 2 (TLR2) agonist activity containing mutant form E7 (E7m) bacterial lipid moiety...
DNA vaccines for infectious diseases and cancer have been explored years. To date, only one vaccine (ZyCoV-D) has authorized emergency use in India. are inexpensive long-term thermostable, however, limited by the low efficiency of intracellular delivery. The recent success mRNA/lipid nanoparticle (LNP) technology coronavirus disease 2019 (COVID-19) pandemic opened a new application nucleic acid-based vaccines. Here, we report that plasmid encoding trimeric spike protein with LNP delivery...
To protect against dengue viral infection, a novel lipidated subunit vaccine was rationally designed to contain the consensus amino acid sequences derived from four serotypes of viruses. We found that virus envelope protein domain III (LcED III) is capable activating antigen-presenting cells and enhancing cellular humoral immune responses. A single-dose LcED immunization in mice without extra adjuvant formulation sufficient elicit neutralizing antibodies all In addition, strong memory...
Background Antigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy to elicit a robust broadened immune response. We assessed the immunogenicity of an inactivated H5N1 whole-virion (A/Vietnam/1194/2004 NIBRG-14, clade 1) formulated with emulsified nanoparticles investigated whether it induce cross-clade protecting immunity. Methodology/Principal Findings After formulation PELC,...
The E7 oncoprotein of human papillomavirus (HPV) is an ideal target for developing immunotherapeutic strategies against HPV-associated tumors. However, because protein-based immunogens alone are poor elicitors the cytotoxic T-lymphocyte (CTL) responses, they have been difficult to exploit therapeutic purposes. In this study, we report that a recombinant lipoprotein consisting inactive (E7m) biologically linked bacterial lipid moiety (rlipo-E7m) induces maturation mouse bone marrow-derived...
Enterovirus 71 (EV71), a positive-stranded RNA virus, is the major cause of hand, foot, and mouth disease (HFMD) with severe neurological symptoms. Antiviral type I interferon (alpha/beta [IFN-α/β]) responses initiated from innate receptor signaling are inhibited by EV71-encoded proteases. It less well understood whether EV71-induced apoptosis provides signal to activate as host defensive mechanism. In this report, we found that EV71 alone cannot Toll-like 9 (TLR9) signaling, but supernatant...
Abstract We developed a novel platform to express high levels of recombinant lipoproteins with intrinsic adjuvant properties. Based on this technology, our group lipidated dengue envelope protein domain IIIs as vaccine candidates against virus. This work aims evaluate the immune responses in mice tetravalent formulation. demonstrate that 4 serotypes III induced both humoral and cellular immunity all virus mixture formed Importantly, by formulation absence exogenous were functional clearing...