A5084853754- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- Influenza Virus Research Studies
- biodegradable polymer synthesis and properties
- Bone Tissue Engineering Materials
- Immune Response and Inflammation
- Immune Cell Function and Interaction
- Advanced Drug Delivery Systems
- Electrospun Nanofibers in Biomedical Applications
- Respiratory viral infections research
- Animal Disease Management and Epidemiology
- Advanced Polymer Synthesis and Characterization
- Nanoparticle-Based Drug Delivery
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 and COVID-19 Research
- Astrophysical Phenomena and Observations
- Orthopaedic implants and arthroplasty
- Mosquito-borne diseases and control
- Graphene and Nanomaterials Applications
- vaccines and immunoinformatics approaches
- T-cell and B-cell Immunology
- Immune cells in cancer
- Additive Manufacturing and 3D Printing Technologies
National Health Research Institutes
2015-2024
China Medical University
2015-2024
Kaohsiung Medical University
2019-2024
National Chung Hsing University
2012-2024
National Astronomical Observatories
2023
Chinese Academy of Sciences
2023
National United University
2018-2020
National Institutes of Health
2013-2015
National Defense Medical Center
2013
National Institute of Infectious Diseases
2013
mRNA vaccines for cancer immunotherapy are commonly delivered using lipid nanoparticles (LNPs), which, when administered intravenously, may accumulate in the liver, potentially limiting their therapeutic efficacy. To overcome this challenge, study introduces an oral vaccine formulation tailored efficient uptake by immune cells gastrointestinal (GI) tract, known its high concentration of cells, including dendritic (DCs). This comprises complexed with β-glucans (βGlus), a potential adjuvant...
Abstract Poly(ϵ‐caprolactone) (PCL) and its block copolymers with poly(ethylene glycol) (PEG) were prepared by ring‐opening polymerization of ϵ‐caprolactone in the presence ethylene glycol or PEG, using zinc metal as catalyst. The resulting polymers characterized various analytical techniques such 1 H NMR, SEC, DSC, IR, X‐ray, ESEM, CZE. PCL/PEG long PCL chains presented same crystalline structure homopolymer, whereas PEG‐bearing short blocks retained PEG exhibited an amphiphilic behavior...
Abstract In the field of tissue engineering new polymers are needed to fabricate scaffolds with specific properties depending on targeted tissue. This work aimed at designing and developing a 3D scaffold variable mechanical strength, fully interconnected porous network, controllable hydrophilicity degradability. For this, desktop-robot-based melt-extrusion rapid prototyping technique was applied novel tri-block co-polymer, namely poly(ethylene...
Synthetic polymers have attracted much attention in tissue engineering due to their ability modulate biomechanical properties. This study investigated the feasibility of processing poly(epsilon-caprolactone) (PCL) homopolymer, PCL-poly(ethylene glycol) (PEG) diblock, and PCL-PEG-PCL triblock copolymers into three-dimensional porous scaffolds. Properties various were by dynamic thermal analysis. The scaffolds manufactured using desktop robot-based rapid prototyping technique. Gross morphology...
The Lobster Eye Imager for Astronomy (LEIA), a pathfinder of the Wide-field X-ray Telescope Einstein Probe (EP) mission, was successfully launched onboard SATech-01 satellite Chinese Academy Sciences on 27 July 2022. In this paper, we introduce design and on-ground test results LEIA instrument. Using state-of-the-art Micro-Pore Optics (MPO), wide field-of-view (FoV) 346 square degrees (18.6 * 18.6 degrees) imager is realized. An optical assembly composed 36 MPO chips used to focus incident...
Abstract Biodegradable copolymers were prepared by ring‐opening polymerization of sequentially added ε ‐caprolactone and DL ‐lactide in the presence ethylene glycol or poly(ethylene glycol), using zinc metal as catalyst. Polymerization was performed bulk yielded block with predetermined PEG/PCL/PLA segments. The obtained polymers characterized 1 H NMR, SEC, IR, DSC, TGA, X‐ray diffraction. Data showed that preserved excellent thermal behavior inherent to PCL. crystallinity PLA‐containing...
Abstract The hydrolytic degradation of various bioresorbable copolymers and blends derived from ϵ‐caprolatcone, D,L ‐lactide poly(ethylene glycol) (PEG) was investigated at 37°C in a pH 7.4 phosphate buffer. Poly(ϵ‐caprolatcone) (PCL) followed slow profile due to its hydrophobicity crystallinity. hydrophilicity degradability the materials can be improved by copolymerization with PEG and/or poly‐( ‐lactide) (PLA). Homogenous shown cases PCL, PCL/PEG their blends, whereas PLA‐containing...
Background The highly pathogenic avian influenza (HPAI) H5N1 virus continues to cause disease in poultry and humans. hemagglutinin (HA) envelope protein is the primary target for subunit vaccine development. Methodology/Principal Findings We used baculovirus-insect cell expression obtain trimeric recombinant HA (rHA) proteins from two HPAI viruses. investigated rHA immunogenicity mice via immunizations, found that highest levels of neutralizing antibodies resulted coupling with a PELC/CpG...
DNA vaccines for infectious diseases and cancer have been explored years. To date, only one vaccine (ZyCoV-D) has authorized emergency use in India. are inexpensive long-term thermostable, however, limited by the low efficiency of intracellular delivery. The recent success mRNA/lipid nanoparticle (LNP) technology coronavirus disease 2019 (COVID-19) pandemic opened a new application nucleic acid-based vaccines. Here, we report that plasmid encoding trimeric spike protein with LNP delivery...
Background Antigen sparing and cross-protective immunity are regarded as crucial in pandemic influenza vaccine development. Both targets can be achieved by adjuvantation strategy to elicit a robust broadened immune response. We assessed the immunogenicity of an inactivated H5N1 whole-virion (A/Vietnam/1194/2004 NIBRG-14, clade 1) formulated with emulsified nanoparticles investigated whether it induce cross-clade protecting immunity. Methodology/Principal Findings After formulation PELC,...
Highly pathogenic avian influenza H5N1 viruses can result in poultry and occasionally human mortality. A safe effective vaccine is urgently needed to reduce the pandemic potential. Hemagglutinin (HA), a major envelope protein accounting for approximately 80% of spikes virus, often used as antigen subunit development. In this study, we conducted systematic study immune response against virus infection following immunization with recombinant HA proteins expressed insect (Sf9) cells, cells that...
Cancer vaccines are considered to be a promising tool for cancer immunotherapy. However, well-designed vaccine should combine tumor-associated antigen (TAA) with the most effective immunomodulatory agents and/or delivery system provoke intense immune responses against TAA. In present study, we introduced new approach by conjugating molecule LD-indolicidin hydrophilic chain end of polymeric emulsifier poly(ethylene glycol)-polylactide (PEG-PLA), allowing located close surface resulting...
Successful DNA vaccination generally requires the aid of either a viral vector within vaccine components or an electroporation device into muscle skin host. However, these systems come with certain obstacles, including limited transgene capacity, broad preexisting immunity in humans, and substantial cell death caused by high voltage pulses, respectively. In this study, we repurposed use amphiphilic bioresorbable copolymer (ABC), called PLA-PEG, as surface engineering agent that conciliates...
Abstract Methylated and pegylated poly(lactide)‐ block ‐poly(ε‐caprolactone)‐ ‐poly(lactide) copolymers, PLA–P(CL‐ co ‐CLCH 3 )–PLA ‐CLPEG)–PLA, were prepared in three steps: combining the formation of carbanion‐bearing dihydroxylated‐PCL, coupling iodomethane or bromoacetylated α‐hydroxyl‐ω‐methoxy‐poly(ethylene glycol) onto carbanionic PCL, finally ring opening polymerization DL ‐lactide initiated by preformed grafted diOH‐PCL copolymers. The resulting copolymers exhibited lower...
PELC is a novel emulsion-type adjuvant that contains the bioresorbable polymer poly (ethylene glycol)-block-poly (lactide-co-ε-caprolactone) (PEG-b-PLACL), Span®85 and squalene. To investigate whether able to enhance CTL responses of antigens for treating tumor, peptides or protein derived from HPV16 E7 were formulated with nanoparticles CpG oligodeoxynucleotide. We identified formulation could delay release in vitro vivo. assessed immunogenicity an H-2D(b)-restricted epitope RAHYNIVTF (RAH)...
The major weaknesses of subunit vaccines are their low immunogenicity and poor efficacy. Adjuvants can help to overcome some these inherent defects with vaccines. Here, we evaluated the efficacy newly developed water-in-oil-in-water multiphase emulsion system, termed PELC, in potentiating protective capacity dengue-1 envelope protein domain III. Unlike aluminum phosphate, III formulated PELC plus CpG oligodeoxynucleotides induced neutralizing antibodies against virus increased splenocyte...
Vaccine adjuvant is conferred on the substance that helps to enhance antigen-specific immune response. Here we investigated disintegration characteristics and immunotherapy potency of an emulsified delivery system comprising bioresorbable polymer poly(ethylene glycol)–polylactide (PEG–PLA), phosphate buffer saline (PBS), metabolizable oil squalane. PEG–PLA-stabilized oil-in-water emulsions show good stability at 4 °C room temperature. At 37 °C, squalane/PEG–PLA/PBS emulsion with oil/aqueous...
Vaccines are a powerful medical intervention for preventing epidemic diseases. Efficient inactivated or protein vaccines typically rely on an effective adjuvant to elicit immune response and boost vaccine activity. In this study, we investigated the activities of combinations Toll-like receptor 9 (TLR9) stimulator interferon genes (STING) agonists in SARS-CoV-2 binding domain vaccine. Adjuvants formulated with TLR9 agonist, CpG-2722, various cyclic dinucleotides (CDNs) that STING increased...
Background Emulsion adjuvants are a potent tool for effective vaccination; however, the size matters on mucosal signatures and mechanism of action following intranasal vaccination remains unclear. Here, we launch mechanistic study to address how membrane interacts with nanoemulsion well-defined at cellular level elucidate impact tumor-associated antigen therapy. Methods The squalene-based emulsified particles submicron/nanoscale could be elaborated by homogenization/extrusion. delivery in...
Abstract The major challenge in COVID‐19 vaccine effectiveness is immune escape by SARS‐CoV‐2 variants. To overcome this, an Omicron‐specific messenger RNA (mRNA) was designed. extracellular domain of the spike Omicron variant fused with a modified GCN4 trimerization low immunogenicity (TSomi). After immunization TSomi mRNA hamsters, animals were challenged virus. raised nonneutralizing antibodies or cytokine secretion responses can recognize both Wuhan S and S. However, neutralized virus...
Abstract Novel emulsion‐type vaccine delivery systems based on the amphiphilic bioresorbable polymer poly(ethylene glycol)‐ block ‐poly(lactide‐ co ‐ε‐caprolactone) (PEG‐ b ‐PLACL) and selected oils were developed here. Physicochemical characterizations such as stability, a droplet test, microscopic aspects, in vitro release showed that PEG‐ ‐PLACL‐emulsified formulations have several advantages over traditional adjuvants they are stable, reproducible, homogeneous fine particles with an...
Thermal properties and physicochemical behavior in aqueous solution of pyrene-labeled poly(ethylene glycol)-polylactide conjugate Wei-Lin Chen,1,2 Yun-Fen Peng,1,3 Sheng-Kuo Chiang,1 Ming-Hsi Huang1–3 1National Institute Infectious Diseases Vaccinology, National Health Research Institutes, Miaoli, Taiwan; 2Graduate Life Sciences, Defense Medical Center, Taipei, 3PhD Program Tissue Engineering Regenerative Medicine, Chung Hsing University, Taichung, Taiwan Abstract: A fluorescence-labeled...
We assessed two strategies for preparing candidate vaccines against hand, foot, and mouth disease (HFMD) caused mainly by infections of enterovirus (EV) 71 coxsackievirus (CV) A16. firstly design optimize the potency adjuvant combinations emulsion-based delivery systems, using EV71 vaccine as a model. then perform immunogenicity studies in mice EV71/CVA16 antigen formulated with PELC/CpG. A single dose inactivated virion (0.2 μ g) emulsified submicron particles was found (i) to induce potent...