A5006191599- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- Influenza Virus Research Studies
- Immunotherapy and Immune Responses
- Animal Disease Management and Epidemiology
- Monoclonal and Polyclonal Antibodies Research
- Bacterial Infections and Vaccines
- Immune Response and Inflammation
- Mosquito-borne diseases and control
- Viral Infections and Outbreaks Research
- Immune Cell Function and Interaction
- Respiratory viral infections research
- Viral Infections and Vectors
- SARS-CoV-2 and COVID-19 Research
- interferon and immune responses
- vaccines and immunoinformatics approaches
- T-cell and B-cell Immunology
- Animal Virus Infections Studies
- RNA Interference and Gene Delivery
- Peptidase Inhibition and Analysis
- HIV Research and Treatment
- Viral Infectious Diseases and Gene Expression in Insects
- Immune cells in cancer
- RNA and protein synthesis mechanisms
- Herpesvirus Infections and Treatments
National Health Research Institutes
2011-2023
China Medical University
2010-2020
National Tsing Hua University
2012-2016
Institute of Immunology
2012-2015
National Defense Medical Center
2015
National Institute of Infectious Diseases
2012-2015
Institut de Recherche Vaccinale
2012-2015
Institute of Life Sciences
2015
National Institutes of Health
2013-2014
Czech Academy of Sciences, Institute of Biotechnology
2012-2013
This study aims to investigate the impact of caregiving on health status and quality life (QOL) primary informal caregivers (PCGs) elderly care recipients in Hong Kong. A total 246 PCGs 492 matched noncaregiver (NCG) controls were identified a population-based cross-sectional through random telephone dialing. Their QOL assessed based structured questionnaires Short Form 36 (SF-36) Health Survey. Multiple conditional logistic regression analysis showed that compared with NCGs, had...
A 43-residue peptide analog of tropomyosin Ac-AB&-OH (A = Lys-Cys-Ala-Glu-Leu-Glu-Gly, B Lys-Leu-Glu-Ala-Leu-Glu-Gly, C Lys-Leu-Glu-Ala-Leu-Glu-Gly-Lys) was synthesized.The 86-residue disulfidelinked dimer prepared by air oxidation the single cysteine residue in NHz-terminal Fragment to provide a two-stranded a-helical coiled-coil defined molecular weight with chains in-register and parallel.The physical properties were determined compared CM-tropomyosin sequential polyheptapeptides.The...
Background Enterovirus 71 (EV71) infections manifest most commonly as a childhood exanthema known hand-foot-and-mouth disease (HFMD) and can cause neurological during acute infection. Principal Finding In this study, we describe the production, purification characterization of EV71 virus produced from Vero cells grown in five-liter serum-free bioreactor system containing 5 g/L Cytodex 1 microcarrier. The viral titer was >106 TCID50/mL by 6 days post infection when MOI 10−5 used at initial...
Mesenchymal stem/stromal cells (MSCs) are promising potential candidates for the treatment of immunological diseases because their immunosuppressive functions. However, molecular mechanisms that mediate MSCs' activity remain elusive. In this article, we report first time, to our knowledge, secreted growth-regulated oncogene (GRO) chemokines, specifically GRO-γ, in human MSC-conditioned media have an effect on differentiation and function monocyte-derived dendritic cells. The were driven...
Background Chinese and Malay subjects have been reported to require less maintenance warfarin than Indians that could not be accounted for by cytochrome P450 (CYP) 2C9 variants. Vitamin K epoxide reductase complex 1 (VKORC1) is the target enzyme of warfarin, VKORC1 intronic variants haplotypes recently shown influence activity requirements. Methods We sequenced coding regions CYP2C9 inferred haplotype from 10 in 147 Chinese, 85 Malay, 43 Indian patients receiving warfarin. Results The mean...
Enterovirus 71 (EV71) and coxsackievirus (CVA) are the most common causative factors for hand, foot, mouth disease (HFMD) neurological disorders in children. Lack of a reliable animal model is an issue investigating EV71-induced manifestation humans, current clinical therapies symptomatic. We generated novel EV71-infectious with hSCARB2-transgenic mice expressing discovered receptor human SCARB2 (hSCARB2). The challenge isolates EV71 CVA16 resulted HFMD-like syndromes caused by E59 (B4)...
Acinetobacter baumannii (Ab) is a global emerging bacterium causing nosocomial infections such as pneumonia, meningitis, bacteremia and soft tissue especially in intensive care units. Since Ab resistant to almost all conventional antibiotics, it now one of the 6 top-priorities dangerous microorganisms listed by Infectious Disease Society America. The development vaccine most promising cost-effective strategies prevent infections. In this study, we identified potential protective candidates...
Enterovirus (EV) 71 infection is known to cause hand-foot-and-mouth disease (HFMD) and in severe cases, induces neurological disorders culminating fatality. An outbreak of EV71 South East Asia 1997 affected over 120,000 people caused a few individuals. The control through public health interventions remains minimal treatments are only symptomatic. Recently, human scavenger receptor class B, member 2 (SCARB2) has been reported be cellular EV71. We expressed SCARB2 gene NIH3T3 cells...
Background The highly pathogenic avian influenza (HPAI) H5N1 virus continues to cause disease in poultry and humans. hemagglutinin (HA) envelope protein is the primary target for subunit vaccine development. Methodology/Principal Findings We used baculovirus-insect cell expression obtain trimeric recombinant HA (rHA) proteins from two HPAI viruses. investigated rHA immunogenicity mice via immunizations, found that highest levels of neutralizing antibodies resulted coupling with a PELC/CpG...
Enterovirus 71 (EV71) has caused several epidemics of hand, foot and mouth diseases (HFMD) in Asia now is being recognized as an important neurotropic virus. Effective medications prophylactic vaccine against EV71 infection are urgently needed. Based on the success inactivated poliovirus vaccine, a prototype chemically candidate been developed currently human phase 1 clinical trial.In this report, we present development serum-free cell-based vaccine. The optimization at each step...
Highly pathogenic avian influenza (HPAI) H5N1 viruses and their transmission capability from birds to humans have raised global concerns about a potential human pandemic. The inherent nature of antigenic changes in has not been sufficiently taken into account immunogen designs for broadly protective HPAI vaccines.We designed hyperglycosylated HA vaccine using N-linked glycan masking on highly variable sequences the HA1 globular head. Immunization these DNA vaccines followed by...