A5023051206- Plant Molecular Biology Research
- Plant Reproductive Biology
- Plant Gene Expression Analysis
- Plant tissue culture and regeneration
- Glutathione Transferases and Polymorphisms
- Redox biology and oxidative stress
- Viral Infections and Immunology Research
- Photosynthetic Processes and Mechanisms
- Retinal Development and Disorders
- Cell Adhesion Molecules Research
- Spaceflight effects on biology
- Photoreceptor and optogenetics research
- Sulfur Compounds in Biology
- RNA regulation and disease
- Ocular and Laser Science Research
- Respiratory viral infections research
- Viral Infectious Diseases and Gene Expression in Insects
- Neonatal Respiratory Health Research
- RNA and protein synthesis mechanisms
- Heat shock proteins research
- Immune Cell Function and Interaction
- Plant nutrient uptake and metabolism
Southern Taiwan Science Park
2013-2024
Academia Sinica
2013-2024
Agricultural Biotechnology Research Center, Academia Sinica
2018-2024
National Health Research Institutes
2011-2013
Enterovirus (EV) 71 infection is known to cause hand-foot-and-mouth disease (HFMD) and in severe cases, induces neurological disorders culminating fatality. An outbreak of EV71 South East Asia 1997 affected over 120,000 people caused a few individuals. The control through public health interventions remains minimal treatments are only symptomatic. Recently, human scavenger receptor class B, member 2 (SCARB2) has been reported be cellular EV71. We expressed SCARB2 gene NIH3T3 cells...
Enterovirus 71 (EV71) causes hand, foot, and mouth disease severe neurological disorders in children. Human scavenger receptor class B member 2 (hSCARB2) P-selectin glycoprotein ligand-1 (PSGL-1) are identified as receptors for EV71. The underling mechanism of PSGL-1-mediated EV71 entry remains unclear. endocytosis required were investigated Jurkat T mouse L929 cells constitutively expressing human PSGL-1 (PSGL-1-L929) or rhabdomyosarcoma (RD) displaying high SCARB2 but no by treatment...
The enigmatic nature of the specialized developmental programs orchids has fascinated plant biologists for centuries. recent releases orchid genomes indicate that possess new gene families, family expansions and contractions to regulate a diverse suite processes. However, extremely long life cycle lack molecular toolkit have hampered advancement biology research. To overcome technical difficulties establish platform rapid regulation studies, in this study, we developed an efficient...
Orchidaceae is one of the most abundant and diverse families in plant kingdom its unique developmental patterns have drawn attention many evolutionary biologists. Particular areas interest included co-evolution pollinators distinct floral structures, symbiotic relationships with mycorrhizal flora. However, comprehensive studies to decipher molecular basis growth development orchids remain scarce. Cell proliferation governed by cell-cycle regulation fundamental body. We took advantage...
Somatic embryogenesis is commonly used for clonal propagation of a wide variety plant species. Induction protocorm-like-bodies (PLBs), which are capable developing into individual plants, routine tissue culture-based practice micropropagation orchid plants. Even though PLBs often regarded as somatic embryos, our recent study provides molecular evidence to argue that not derived from embryogenesis. Here, we report and characterize the embryonic tissues induced by Phalaenopsis aphrodite LEAFY...
The protocorm is a structure that formed upon germination of an orchid seed. It lacks cotyledons and ovoid in shape. protocorm-like body (PLB), on the other hand, organ induced from somatic tissues. PLBs have been widely used for micropropagation. Because its unique application industry, PLB development has drawn considerable interest developmental biologists. Our previous genome-wide comparative transcriptome study demonstrated protocorms share similar molecular signatures suggested SHOOT...
Identification of HLA-restricted CD8+ T cell epitopes is important to study RSV-induced immunity and illness. We algorithmically analyzed the sequence fusion protein (F) respiratory syncytial virus (RSV) generated synthetic peptides that can potentially bind HLA-A*0201. Four out twenty-five 9-mer tested: 3 (F33–41), 13 (F214–222), 14 (F273–281), 23 (F559–567), were found HLA-A*0201 with moderate high affinity capable inducing IFN-γ IL-2 secretion in lymphocytes from transgenic (HLA-Tg) mice...
Abstract In epiphytes, aerial roots are important to combat water‐deficient, nutrient‐poor, and high‐irradiance microhabitats. However, whether can respond gravity auxin plays a role in regulating root development remain open‐ended questions. Here, we investigated the gravitropic response of epiphytic orchid Phalaenopsis aphrodite . Our data showed that P. failed gravity, this was correlated with lack starch granules/statolith sedimentation absence efflux carrier PIN2 gene. Using an...
Glutathione redox cycling is important for cell cycle regulation, but its mechanisms are not well understood. We previously identified a small-sized mutant, suppressor of mat3 15-1 (smt15-1) that has elevated cellular glutathione. Here, we demonstrated SMT15 chloroplast sulphate transporter. Reducing expression γ-GLUTAMYLCYSTEINE SYNTHETASE, encoding the rate-limiting enzyme required glutathione biosynthesis, corrected size defect smt15-1 cells. Overexpressing GLUTATHIONE SYNTHETASE (GSH2)...
Abstract Glutathione redox cycling is important for cell cycle regulation. However, the underlying mechanisms remain unclear. We previously identified a cell-size mutant, suppressor of mat3 15-1 ( smt15-1 ), that has elevated cellular glutathione, increased number divisions, and small daughter cells. Here, we demonstrated SMT15 chloroplast-associated membrane protein capable transporting sulfate. Reducing expression γ-GLUTAMYLCYSTEINE SYNTHETASE , which encodes rate-limiting enzyme required...