A5087776121- Advanced Fluorescence Microscopy Techniques
- Optical Coherence Tomography Applications
- Photoacoustic and Ultrasonic Imaging
- Dermatology and Skin Diseases
- melanin and skin pigmentation
- Skin Protection and Aging
- Advancements in Transdermal Drug Delivery
- Cell Adhesion Molecules Research
- Psoriasis: Treatment and Pathogenesis
- Skin and Cellular Biology Research
- Ocular Infections and Treatments
- MicroRNA in disease regulation
- Genetic and rare skin diseases.
- Contact Dermatitis and Allergies
- Spectroscopy Techniques in Biomedical and Chemical Research
- Nonmelanoma Skin Cancer Studies
- Corneal surgery and disorders
- Hair Growth and Disorders
- Tendon Structure and Treatment
- CAR-T cell therapy research
- Retinoids in leukemia and cellular processes
- Cancer and Skin Lesions
- Cutaneous lymphoproliferative disorders research
- Silk-based biomaterials and applications
- Lipid Membrane Structure and Behavior
National Taiwan University
2005-2023
National Taiwan University Hospital
2011-2021
Cathay General Hospital
2016-2021
Hanyang University Guri Hospital
2010
National Science and Technology Council
2006
Academia Sinica
2006
University of California, San Francisco
2000
Chang Gung Memorial Hospital
1998
Mayo Clinic
1995
Taipei Hospital
1980-1994
The anticonvulsant carbamazepine (CBZ) frequently causes cutaneous adverse drug reactions (cADRs), including maculopapular eruption (MPE), hypersensitivity syndrome (HSS), Stevens–Johnson (SJS) and toxic epidermal necrolysis (TEN). We reported that SJS/TEN caused by CBZ is strongly associated with the HLA-B*1502 gene in Han Chinese. Here, we extended our genetic study to different types of CBZ-cADRs (91 patients, 60 patients SJS/TEN, 13 18 exanthema versus 144 tolerant controls). used...
The photoaging process of facial skin is investigated by use multiphoton fluorescence and second-harmonic generation (SHG) microscopy. We obtain the autofluorescence (AF) SHG images superficial dermis from three patients aged 20, 40, 70? years. results show that areas AF increase with age, whereas decrease age. are consistent histological findings in which collagen progressively replaced elastic fibers. changes quantified a to aging index (SAAID). Our suggest SAAID can be good indicator...
Background One determining factor of skin colour is the distribution pattern melanosomes within keratinocytes. Melanosomes in keratinocytes light as Caucasians are distributed membrane-bound clusters, whereas dark African/American individuals tend to be larger and individually. It has been shown that melanin content, composition size human epidermis vary considerably with both ethnicity chronic sun exposure. Objectives To assess quantitatively have transferred photoprotected (volar forearm)...
Hypoxia-inducible factor-1alpha (HIF-1alpha) is widely considered to be one of the key regulators tumor angiogenesis. The upstream regulation complex and involves several growth factors, cytokines, hypoxia. Herein, we have identified miR-519c as a hypoxia-independent regulator HIF-1alpha, acting through direct binding HIF-1alpha 3' untranslated region leading reduced Overexpression resulted in significant decrease protein levels tube formation human umbilical vein endothelial cells;...
We performed multiphoton fluorescence (MF) and second-harmonic generation (SHG) imaging on human basal cell carcinoma samples. In the dermis, carcinomas can be identified by masses of autofluorescent cells with relatively large nuclei marked peripheral palisading. normal SHG from dermal collagen contributes largely to signal. However, within cancer stroma, signals diminish are replaced signals, indicating that structures responsible for have been altered. To better delineate stroma a...
Color patterns of bird plumage affect animal behavior and speciation. Diverse are present in different species within the individual. Here, we study cellular molecular basis feather pigment pattern formation. Melanocyte progenitors distributed as a horizontal ring proximal follicle, sending melanocytes vertically up into epithelial cylinder, which gradually emerges feathers grow. Different form by modulating presence, arrangement, or differentiation melanocytes. A layer peripheral pulp...
The purpose of this work was to demonstrate the use combined imaging modality multiphoton autofluorescence and second-harmonic generation (SHG) microscopy in obtaining spectrally resolved morphologic features cornea, limbus, conjunctiva, sclera whole, ex vivo porcine eyes.The 780-nm output a femtosecond, titanium-sapphire laser used induce broadband (435-700 nm) SHG (390 from various regions surface eyes. A water-immersion objective for convenient curved eye.Multiphoton useful identifying...
Background Pruritus is very common in uraemic patients, but the treatment remains challenging. Studies regarding narrowband ultraviolet B (NB-UVB) phototherapy for pruritus are rare. Objectives To investigate whether or not NB-UVB an effective pruritus. Methods We conducted a single-blind, randomized, controlled trial patients with refractory The group received three times per week 6 weeks. dose of started from 210 mJ cm−2 and was increased by 10% each time. control time-matched exposures to...
Background Uremic pruritus is a common and intractable symptom in patients on chronic hemodialysis, but factors associated with the severity of remain unclear. This study aimed to explore associations metabolic dialysis adequacy aggravation pruritus. Methods We conducted 5-year prospective cohort maintenance hemodialysis. A visual analogue scale (VAS) was used assess intensity Patient demographic clinical characteristics, laboratory parameters, (assessed by Kt/V), were recorded at baseline...
To demonstrate the application of multiphoton fluorescence (MF) and second harmonic generation (SHG) microscopy for ex vivo characterization structural alterations human corneas due to keratoconus.Buttons keratoconic derived from penetrating keratoplasty were sent analysis with a custom-built microscope. Fluorescence detected within cytoplasm SHG signal obtained collagen used morphologic changes in corneal specimens.The fluorescent epithelial cells around apical area elongated aligned...
The thermal disruption of collagen I in rat tail tendon is investigated with second-harmonic generation (SHG) microscopy. We investigate its effects on SHG images and intensity the temperature range 25 degrees-60 degrees C. find that signal decreases rapidly starting at 45 However, imaging reveals breakage fibers not evident until 57 C worsens increasing temperature. At C, structures both molten fibrous exist, appears to be complete 60 Our results suggest that, addition measurement,...
Although pruritus is a common complaint in patients with diabetes, little known about its relation glycemic control. We investigated whether generalized associated control type 2 diabetes. A total of 385 diabetes who attended the care system underwent cutaneous examination by dermatologist at teaching hospital Taiwan. detailed interview questionnaire including visual analogue scale was used to assess various characteristics and intensity pruritus. Multivariate logistic regression association...