A5010918611- Antioxidant Activity and Oxidative Stress
- Atherosclerosis and Cardiovascular Diseases
- Eicosanoids and Hypertension Pharmacology
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Lipoproteins and Cardiovascular Health
- Fatty Acid Research and Health
- Redox biology and oxidative stress
- Skin Protection and Aging
- Wnt/β-catenin signaling in development and cancer
- Immune Response and Inflammation
- Metabolomics and Mass Spectrometry Studies
- Mitochondrial Function and Pathology
- Natural product bioactivities and synthesis
- Phagocytosis and Immune Regulation
- Sphingolipid Metabolism and Signaling
- Peroxisome Proliferator-Activated Receptors
- Genomics, phytochemicals, and oxidative stress
- Cancer-related gene regulation
- Cholesterol and Lipid Metabolism
- Caveolin-1 and cellular processes
- Pharmacological Effects of Natural Compounds
- Adipose Tissue and Metabolism
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Nitric Oxide and Endothelin Effects
- Cancer, Lipids, and Metabolism
University of Graz
2016-2025
Medical University of Vienna
2007-2018
National Jewish Health
2017
National Cancer Institute
2017
University of Colorado Denver
2017
Thrombosis Research Institute
2015
University of Vienna
2002-2011
University Hospital of Basel
1992-2010
University of Basel
2010
University of Eastern Finland
2008
Heme oxygenase-1 (HO-1) catalyzes the rate-limiting step in heme degradation, protects against oxidative stress, and shows potent anti-inflammatory effects. Oxidized phospholipids, which are generated during inflammation apoptosis, modulate inflammatory response by inducing expression of several genes including HO-1. Here we investigated signaling pathways transcriptional events involved induction HO-1 gene oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (OxPAPC) human...
Angiogenesis is a common feature observed in advanced atherosclerotic lesions. We hypothesized that oxidized phospholipids (OxPLs), which accumulate vessels can stimulate angiogenesis. found 1-palmitoyl-2-arachidonoyl- sn -glycero-3-phosphocholine (OxPAPC) stimulated the formation of sprouts from endothelial cell spheroids and promoted growth capillaries into Matrigel plugs mice. OxPLs expression vascular factor (VEGF) vivo several normal tumor types vitro. In addition, OxPAPC upregulated...
Besides their well-characterized proinflammatory and proatherogenic effects, oxidized phospholipids, such as oxPAPC (oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-phosphocholine) have been shown to beneficial responses in vascular cells via induction of antioxidant enzymes heme oxygenase-1. We therefore hypothesized that could evoke a general cytoprotective response activation antioxidative transcription factor Nrf2. Here, we show increases nuclear accumulation Using the small interfering...
The ATF4 arm of the unfolded protein response is increasingly recognized for its relevance to pathology, and in particular angiogenic reactions. Oxidized phospholipids (OxPLs), known accumulate atherosclerotic vessels, were shown upregulate vascular endothelial growth factor (VEGF) induce angiogenesis via an ATF4-dependent mechanism. In this study, we analyzed mechanism upregulation by OxPLs more specifically involvement NRF2, major transcriptional mediator electrophilic stress...
DCs are able to undergo rapid maturation, which subsequently allows them initiate and orchestrate T cell-driven immune responses. DC maturation must be tightly controlled in order avoid random cell activation development of autoimmunity. Here, we determined that 12/15-lipoxygenase-meditated (12/15-LO-mediated) enzymatic lipid oxidation regulates fine-tunes consecutive Specifically, 12/15-LO activity the threshold via generation phospholipid products induced an antioxidative response...
After an acute phase of inflammation or injury, restoration the endothelial barrier is important to regain vascular integrity and prevent edema formation. However, little known about mediators that control function. We show here oxidized phospholipids accumulate at sites tissue damage are potent regulators Oxygenated epoxyisoprostane-containing phospholipids, but not fragmented exhibited barrier-protective effects mediated by small GTPases Cdc42 Rac their cytoskeletal, focal adhesion,...
Lipoprotein-derived phospholipid oxidation products have been implicated as candidate triggers of the inflammatory process in atherosclerosis. However, vivo evidence regarding impact oxidized phospholipids on artery wall thus far has elusive. Therefore, aim this study was to investigate if structurally defined induce expression atherogenic chemokines and monocyte adhesion intact murine arteries.To model accumulation arterial wall, 1-palmitoyl-2-arachidonoyl-sn-3-glycero-phosphorylcholine...
Acute inflammation and vascular leak are cardinal features of acute lung injury the respiratory distress syndrome. Nonspecific tissue in response to infectious noninfectious insults lead oxidative stress generation lipid oxidation products, which may inhibit inflammatory bacterial components.To test hypothesis that oxidized 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphorylcholine (OxPAPC) attenuate lipopolysaccharide (LPS) enhance barrier recovery, we used vivo vitro models LPS-induced...
Polyunsaturated fatty acids are precursors of multiple pro- and anti-inflammatory molecules generated by enzymatic stereospecific positionally specific insertion oxygen, which is a prerequisite for recognition these mediators cellular receptors. However, nonenzymatically oxidized free esterified polyunsaturated also demonstrate activities relevant to inflammation. In particular, phospholipids containing acid residues (oxidized phospholipids; OxPLs) were shown induce proinflammatory changes...
Oxidized phospholipids (OxPLs) that are abundant in atherosclerotic lesions increasingly recognized as context-dependent lipid mediators demonstrating both pro- and antiinflammatory activities. Molecular mechanisms of their effects largely unknown. Here we present novel information on the whereby OxPLs modulate activation TLR4 by lipopolysaccharide (LPS).We show, using several cell types various inflammatory genes readouts, different classes molecular species do not stimulate but exert...
Oxidized phospholipids (OxPLs) are increasingly recognized as signaling mediators that not only markers of oxidative stress but also "makers" pathology relevant to disease pathogenesis. Understanding the biological role individual molecular species OxPLs requires knowledge their concentration kinetics in cells and tissues. In this work, we describe a straightforward "fingerprinting" procedure for analysis broad spectrum generated by oxidation four most abundant polyunsaturated...
Vascular integrity and the maintenance of blood vessel continuity are fundamental features circulatory system maintained through endothelial cell-cell junctions. Defects in barrier become an initiating factor several pathologies, including ischemia/reperfusion, tumor angiogenesis, pulmonary edema, sepsis, acute lung injury. Better understanding mechanisms stimulating enhancement may provide novel therapeutic strategies. We previously reported that oxidized phospholipids (oxidized...
Cadherins are a family of cellular adhesion proteins mediating homotypic cell‐cell binding. In contrast to classical cadherins, T‐cadherin does not possess the transmembrane and cytosolic domains known be essential for tight mechanical coupling cells, is instead attached cell membrane by glycosylphosphatidylinositol (GPI) anchor. This study explores hypothesis that might function as signal‐transducing protein. Membranes from human rat vascular smooth muscle cells were fractionated using...
Lipid oxidation products and in particular oxidized phospholipids (OxPL) are increasingly recognized as inducers of chronic inflammation characteristic atherosclerosis. OxPL stimulate production chemokines adhesion monocytes to endothelial cells. However, accumulating data suggest that, addition the proatherogenic proinflammatory effects, can antiinflammatory tissue-protective mechanisms. Thus, depending on biological situation, either or inhibit inflammation. In this review, inflammatory...