A5050421555- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Immune Response and Inflammation
- RNA Interference and Gene Delivery
- Corneal Surgery and Treatments
- Atherosclerosis and Cardiovascular Diseases
- Ocular Surface and Contact Lens
- Corneal surgery and disorders
- Immune cells in cancer
- Peptidase Inhibition and Analysis
- Mast cells and histamine
- Bone Metabolism and Diseases
- Phagocytosis and Immune Regulation
- Extracellular vesicles in disease
- Ubiquitin and proteasome pathways
- Cytomegalovirus and herpesvirus research
- Signaling Pathways in Disease
- Antimicrobial Peptides and Activities
- MicroRNA in disease regulation
- vaccines and immunoinformatics approaches
- Genomics, phytochemicals, and oxidative stress
- Renal Transplantation Outcomes and Treatments
- Transgenic Plants and Applications
Universitätsklinikum Erlangen
2014-2023
Friedrich-Alexander-Universität Erlangen-Nürnberg
2002-2023
University of Agriculture Faisalabad
1992
DCs are able to undergo rapid maturation, which subsequently allows them initiate and orchestrate T cell-driven immune responses. DC maturation must be tightly controlled in order avoid random cell activation development of autoimmunity. Here, we determined that 12/15-lipoxygenase-meditated (12/15-LO-mediated) enzymatic lipid oxidation regulates fine-tunes consecutive Specifically, 12/15-LO activity the threshold via generation phospholipid products induced an antioxidative response...
Bioactive borate glasses additionally doped with copper and/or zinc have enthralling immune-modulatory effects on immune cells.
Excessive macrophage (Mφ) activation results in chronic inflammatory responses or autoimmune diseases. Therefore, identification of novel immune checkpoints on Mφ, which contribute to resolution inflammation, is crucial for the development new therapeutic agents. Herein, we identify CD83 as a marker IL-4 stimulated pro-resolving alternatively activated Mφ (AAM). Using conditional KO mouse (cKO), show that important phenotype and function Mφ. CD83-deletion decreased levels inhibitory...
CD83 is up-regulated on the surface of dendritic cells (DCs) during maturation and has been widely used as a marker for mature DCs. Recently, we reported recombinant expression extracellular immunoglobulin domain human (hCD83ext). Using this soluble form CD83, allogeneic well specific cytotoxic T lymphocyte proliferation could be blocked in vitro. Here report functional analysis vivo, using murine experimental autoimmune encephalomyelitis (EAE) model. Strikingly, only three injections...
Modulation of immune responses is one the main research aims in transplant immunology. In this study, we investigate local immunomodulatory properties soluble CD83 (sCD83) at graft-host interface using high-risk corneal transplantation model. model, which mimics inflammatory status and preexisting vascularization patients undergoing transplantation, allogeneic donor corneas are transplanted onto sCD83-treated recipient animals. This model allows direct precise application modulator side....
Foxp3-positive regulatory T cells (Tregs) are crucial for the maintenance of immune homeostasis and keep responses in check. Upon activation, Tregs transferred into an effector state expressing transcripts essential their suppressive activity, migration, survival. However, it is not completely understood how different intrinsic environmental factors control differentiation. Here, we present first time to our knowledge data suggesting that Treg-intrinsic expression CD83 Treg differentiation...
Dendritic cells (DCs) are crucial regulators of immunity and important in inducing maintaining tolerance. Here, we investigated the potential a novel DC-immunomodulating agent, soluble CD83 (sCD83), transplant tolerance.We used C3H-to-C57BL/6 mouse cardiac transplantation model that exhibits combination severe cell-mediated rejection moderate antibody-mediated whether sCD83 could augment therapy consisting Rapamycin (Rapa) anti-CD45RB monoclonal antibody (α-CD45) to prolong allograft...
Dendritic cells (DCs) are crucial to balance protective immunity and autoimmune inflammatory processes. Expression of CD83 is a well-established marker for mature DCs, although its physiological role still not completely understood. Using DC-specific CD83–conditional KO (CD83ΔDC) mouse, we provide new insights into the function within this cell type. Interestingly, CD83-deficient DCs produced drastically increased IL-2 levels displayed higher expression costimulatory molecules CD25 OX40L,...
The aryl hydrocarbon receptor (AhR) is an environmental sensor and ligand-activated transcription factor that critically involved in the regulation of inflammatory responses induction tolerance by modulating immune cells. As dendritic cells (DCs) express high AhR levels, they are efficient to induce immunomodulatory effects after being exposed AhR-activating compounds derived from environment or diet. To gain new insights into molecular targets following AhR-activation human monocyte-derived...
NFATc1 is a member of the nuclear factor activated T cells (NFAT) family transcription factors. NFAT upon T‐cell receptor activation followed by intracytoplasmatic calcium influx where calmodulin, sensor protein, activates phosphatase calcineurin that dephosphorylates proteins and results in import. Here, we show analysis conditional NFATc1‐deficient mice bearing deletion CD4 + CD8 cells. polarized under Th17 conditions express reduced levels Th17‐associated RORγT (where ROR RAR‐related...
Interference with autoimmune-mediated cytokine production is a key yet poorly developed approach to treat autoimmune and inflammatory diseases such as rheumatoid arthritis. Herein, we show that soluble CD83 (sCD83) enhances the resolution of antigen-induced arthritis (AIA) by strongly reducing expression levels cytokines IL-17A, IFNγ, IL-6, TNFα within joints. Noteworthy, also RANKL, osteoclast differentiation, joint destruction was significantly inhibited sCD83. In addition, osteoclasts...
Plasma extracellular vesicles (pEV) can harbor a diverse array of factors including active proteases and the amyloid-precursor-protein (APP) cleavage product Aβ, involved in plaque formation Alzheimer`s diseases (AD). A potential role such AD pathology is unexplored. In case-control study randomly selected patients with other neurological (n = 14), healthy controls 7), we systematically analyzed content pEV, using different assay systems. addition, determined their entry path into brain...
During inflammation, murine and human monocytes can develop into dendritic cells (DC), but this process is not entirely understood. Here, we demonstrate that extracellular vesicles (EV) secreted by mature DC (maDC) differentiate peripheral immature DC, expressing a unique marker pattern, including 6-sulfo LacNAc (slan), Zbtb46, CD64, CD14. While EV from both maDC differentiated similar to GM-CSF/IL-4 stimulation, only maDC-EV produced precursors, which upon maturation stimulus developed...
Abstract The small adaptor protein growth factor receptor–bound 2 (Grb2) modulates and integrates signals from receptors on cellular surfaces in inner signaling pathways. In murine T cells, Grb2 is crucial for amplification of TCR signaling. cell–specific Grb2fl/fl Lckcretg Grb2-deficient mice show reduced cell numbers due to impaired negative positive selection. this study, we found that CD4cretg were normal the thymus only slightly affected periphery. Ex vivo analysis CD4+ Th populations...
Immune responses reflect a complex interplay of cellular and extracellular components which define the microenvironment tissue. Therefore, factors that locally influence re-establish tolerance might be beneficial to mitigate immune-mediated reactions, including rejection transplant. In this study, we demonstrate pre-incubation donor tissue with immune modulator soluble CD83 (sCD83) significantly improves graft survival using high-risk corneal transplantation model. The induction tolerogenic...