A5085503776- Immune cells in cancer
- Mesenchymal stem cell research
- Peroxisome Proliferator-Activated Receptors
- Nuclear Receptors and Signaling
- Monoclonal and Polyclonal Antibodies Research
- Bone Metabolism and Diseases
- Phagocytosis and Immune Regulation
- Macrophage Migration Inhibitory Factor
- Immune Cell Function and Interaction
- Immune Response and Inflammation
- Bone and Joint Diseases
- Cell death mechanisms and regulation
- Trace Elements in Health
- Bone health and treatments
- Cancer, Hypoxia, and Metabolism
- Cell Adhesion Molecules Research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Cancer Cells and Metastasis
- Inflammatory mediators and NSAID effects
- T-cell and B-cell Immunology
- Inflammasome and immune disorders
- Thermal Regulation in Medicine
- Zebrafish Biomedical Research Applications
- Neuroinflammation and Neurodegeneration Mechanisms
- Hippo pathway signaling and YAP/TAZ
Cardiff University
2016-2024
University of Wales Institute Cardiff
2022
Friedrich-Alexander-Universität Erlangen-Nürnberg
2012-2021
Universitätsklinikum Erlangen
2012-2021
Tenovus Cancer Care
2019
Fraunhofer Institute for Cell Therapy and Immunology
2014
DCs are able to undergo rapid maturation, which subsequently allows them initiate and orchestrate T cell-driven immune responses. DC maturation must be tightly controlled in order avoid random cell activation development of autoimmunity. Here, we determined that 12/15-lipoxygenase-meditated (12/15-LO-mediated) enzymatic lipid oxidation regulates fine-tunes consecutive Specifically, 12/15-LO activity the threshold via generation phospholipid products induced an antioxidative response...
Uptake of apoptotic cells (ACs) by macrophages ensures the nonimmunogenic clearance dying cells, as well maintenance self-tolerance to AC-derived autoantigens. Upon ingestion, ACs exert an inhibitory influence on inflammatory signaling within phagocyte. However, molecular signals that mediate these immune-modulatory properties are incompletely understood. In this article, we show phagocytosis thymocytes was enhanced in tissue-resident where process resulted inhibition NF-κB and repression...
Article2 June 2020Open Access Transparent process Tissue-resident macrophages actively suppress IL-1beta release via a reactive prostanoid/IL-10 pathway Natacha Ipseiz Systems Immunity Research Institute, Heath Park, Cardiff University, Cardiff, UK Search for more papers by this author Robert J Pickering orcid.org/0000-0003-3332-9868 Marcela Rosas Victoria Tyrrell Luke C Davies Selinda Orr Wellcome-Wolfson Institute Experimental Medicine, School of Dentistry and Biomedical Science, Queen's...
Abstract Efficient cellular fusion of mononuclear precursors is the prerequisite for generation fully functional multinucleated bone-resorbing osteoclasts. However, exact molecular factors and mechanisms controlling osteoclast remain incompletely understood. Here we identify RANKL-mediated activation caspase-8 as early key event during fusion. Single cell RNA sequencing-based analyses suggested that parts apoptotic machinery accompanied differentiation into mature A subsequent...
Rapid clearance of apoptotic cells, frequently referred to as efferocytosis, is crucial for the maintenance tissue homeostasis and prevention autoimmunity. The common model cell involves a system released "Find me" exposed "Eat signals on detected recognized by matching receptors macrophages or dendritic cells (DC), phagocytic synapse. Osteoclasts share monocyte lineage with these professional mononuclear phagocytes, thus raising question if, in addition bone resorption, osteoclasts can act...
To define how peroxisome proliferator-activated receptor (PPAR) β/δ expression level in mesenchymal stem cells (MSCs) could predict and direct both their immunosuppressive therapeutic properties. PPARβ/δ interacts with factors such as nuclear factor-kappa B (NF-κB) regulates the of molecules including vascular cell adhesion molecule (VCAM)-1 intercellular (ICAM)-1. Since these are critical for MSC function, we investigated role on properties.We either treated human MSCs (hMSCs) irreversible...
Apart from their role in the immune defence against pathogens evidence of a antimicrobial peptides (AMPs) autoimmune diseases has accumulated past years. The aim this project was to examine functional impact human cathelicidin LL-37 and mouse cathelicidin-related AMP (CRAMP) on pathogenesis lupus arthritis. Serum anti-LL-37 levels were measured by ELISA healthy donors patients with Systemic Lupus Erythematosus (SLE) Rheumatoid arthritis (RA). Pristane-induced induced female wild type (WT)...
Abstract Microglia cells fulfill key homeostatic functions and essentially contribute to host defense within the CNS. Altered activation of microglia, in turn, has been implicated neuroinflammatory neurodegenerative diseases. In this study, we identify nuclear receptor (NR) Nr4a1 as rheostat controlling threshold polarization microglia. steady-state ubiquitous neuronal-derived stress signals such ATP induced expression NR, which contributed maintenance a resting noninflammatory microglia...
Eosinophils were reported to serve as an essential component of the plasma cell niche within bone marrow. As potential contribution eosinophils humoral immunity has remained incompletely understood, we aimed further characterize their role during antibody responses and additionally investigate in autoimmune disease. Contrary our expectations currently prevailing paradigm, found that are fully dispensable for survival murine marrow cells accordingly do not contribute production...
ABSTRACT NR4A1 (Nur77 or NGFI-B), an orphan member of the nuclear receptor superfamily, has been identified as a key regulator differentiation and function myeloid, lymphoid, mesenchymal cells. The detailed role in bone biology is incompletely understood. Here, we report for novel factor controlling migration recruitment osteoclast precursors during remodeling. Myeloid-specific but not osteoblast-specific deletion resulted osteopenia due to increase number bone-lining osteoclasts. Although...
Abstract Bone turnover, which is determined by osteoclast-mediated bone resorption and osteoblast-mediated formation, represents a highly energy consuming process. The metabolic requirements of osteoblast differentiation mineralization, both essential for regular however, remain incompletely understood. Here we identify the nuclear receptor peroxisome proliferator-activated (PPAR) δ as key regulator metabolism. Induction PPARδ was adaption increased rate in mitochondrial respiration...
Mesenchymal stem cells (MSC) are highly immunosuppressive able to reduce chronic inflammation through the active release of mediators.Recently, we showed that glucocorticoid-induced leucine zipper (Gilz) expression by MSC is involved in their therapeutic effect promoting generation regulatory T cells.However, mechanisms underlying this pivotal role Gilz remain elusive. Methods and ResultsIn study, have uncovered evidence modulates phenotype function Th1 Th17 likely upregulating level Activin...
Tissue-resident macrophages exhibit specialized phenotypes dependent on their in vivo physiological niche. Investigation of function often relies upon complex whole mouse transgenic studies. While some appropriate lineage-associated promoters exist, there are no options for tissue-specific targeting macrophages. We have developed full protocols productive infection (defined by stable transgene expression) tissue-resident with lentiviral vectors, enabling RNA and protein overexpression,...
Chronic Kidney Disease (CKD) is associated with markedly increased cardiovascular (CV) morbidity and mortality. inflammation, a hallmark of both CKD CV diseases (CVD), believed to drive this association. Pro-inflammatory endogenous TLR agonists, Damage-Associated Molecular Patterns (DAMPs), have been found elevated in patients’ plasma suggested promote CVD, however, confirmation their involvement, the underlying mechanism(s), extent which individual DAMPs contribute vascular pathology...
Inflammatory responses require mobilization of innate immune cells from the bone marrow. The functionality this process depends on state marrow microenvironment. We therefore hypothesized that molecular changes in osteoblasts, which are essential stromal microenvironment, influence inflammatory response. Here, we show osteoblast-specific expression AP-1 transcription factor Fra-2 (Fra-2Ob-tet) induced a systemic with infiltration neutrophils and proinflammatory macrophages into spleen liver...