A5017601607- Cancer, Hypoxia, and Metabolism
- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Adipose Tissue and Metabolism
- Metabolism and Genetic Disorders
- Autophagy in Disease and Therapy
- Metabolism, Diabetes, and Cancer
- Pancreatic function and diabetes
- Hormonal Regulation and Hypertension
- Genetics and Neurodevelopmental Disorders
- Ubiquitin and proteasome pathways
- ATP Synthase and ATPases Research
- Angiogenesis and VEGF in Cancer
- Diet and metabolism studies
- Adrenal Hormones and Disorders
- Growth Hormone and Insulin-like Growth Factors
- Cancer-related Molecular Pathways
- Nanoparticles: synthesis and applications
- Circadian rhythm and melatonin
- Immune Response and Inflammation
- RNA modifications and cancer
- Liver Disease Diagnosis and Treatment
- Cell Adhesion Molecules Research
- RNA regulation and disease
- RNA Interference and Gene Delivery
National Academy of Sciences of Ukraine
2016-2025
Palladin Institute of Biochemistry
2016-2025
Biology Institute of Shandong Academy of Sciences
2022
Bogomolets National Medical University
2015-2017
Université de Bordeaux
2012
Inserm
2012
Medical University of Vienna
2006
University Hospital of Basel
2006
University of Virginia
2006
Luzerner Kantonsspital
2006
Vascular endothelial growth factor (VEGF) mediates angiogenic activity in a variety of estrogen target tissues. To determine whether has direct transcriptional effect on VEGF gene expression, we developed model system by transiently transfecting human promoter-luciferase reporter constructs into primary endometrial cells and Ishikawa cells, derived from well-differentiated adenocarcinoma. In epithelial treatment with 17β-estradiol (E 2 ) resulted 3.8-fold increase luciferase activity,...
One of the key mediators hypoxic response in animal cells is hypoxia-inducible transcription factor-1 (HIF-1) complex, which α-subunit highly susceptible to oxygen-dependent degradation. The manifested many pathophysiological processes such as tumor growth and metastasis. During hypoxia, shift a primarily glycolytic metabolic mode for their energetic needs. This also HIF-1-dependent up-regulation genes. Paradoxically, growing under conditions normal oxygen tension show elevated rates that...
When oxygen becomes limiting, cells shift primarily to a glycolytic mode for generation of energy. A key regulator flux is fructose‐2,6‐bisphosphate (F‐2,6‐BP), potent allosteric 6‐phosphofructo‐1‐kinase (PFK‐1). The levels F‐2,6‐BP are maintained by family bifunctional enzymes, 6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase (PFKFB or PFK‐2), which have both kinase and phosphatase activities. Each member the enzyme characterized their phosphatase:kinase activity ratio (K:B)...
The study addressed the role for aldose reductase (AR) in 1) retinal oxidative stress and vascular endothelial growth factor (VEGF) overexpression early diabetes, 2) high glucose-induced cells. In vivo experiments were performed on control rats diabetic treated with or without low dose of AR inhibitor (ARI) fidarestat (2 16 mg · kg−1 day−1). vitro studies bovine cells (BREC) cultured either 5 30 mmol/l glucose 1 μmol/l fidarestat. Intracellular reactive oxygen species assessed using...
Angiogenesis is a common feature observed in advanced atherosclerotic lesions. We hypothesized that oxidized phospholipids (OxPLs), which accumulate vessels can stimulate angiogenesis. found 1-palmitoyl-2-arachidonoyl- sn -glycero-3-phosphocholine (OxPAPC) stimulated the formation of sprouts from endothelial cell spheroids and promoted growth capillaries into Matrigel plugs mice. OxPLs expression vascular factor (VEGF) vivo several normal tumor types vitro. In addition, OxPAPC upregulated...
Hypoxia induces the stabilization and transcriptional activation of hypoxia-inducible factor 1α (HIF-1α) protein, regulatory member HIF-1 complex. The molecular mechanisms that are responsible for oxygen sensing downstream pathways utilized by hypoxic signal still poorly understood. One hypothesis has postulated reactive species generated at mitochondrial complex III initiators signal. Here we find DNA-less (ρo) cells have a normal response to hypoxia, measured level HIF-1α protein...
Abstract Background Epidermal growth factor (EGF) receptors contribute to the development of malignant glioma. Here we considered possible implication EGFR ligand epiregulin (EREG) in glioma relation activity unfolded protein response (UPR) sensor IRE1α. We also examined EREG status several glioblastoma cell lines and Methods Expression biological properties were analyzed human cells vitro tumor xenografts with regard presence ErbB proteins blockade Inactivation IRE1α was achieved by using...
The PFKFB4 gene encodes isoenzyme of 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase (PFKFB or PFK-2/FBPase-2) which originally was found in the testes. We have studied hypoxic regulation prostate cancer cell line, PC-3, and several other lines. It shown that hypoxia significantly induced mRNA levels PC-3 as well HeLa, Hep3B HepG2 Hypoxia increased protein also. Moreover, desferrioxamine cobalt chloride, are known to mimic hypoxia, also had a stimulatory effect on expression mRNA. In...
P-selectin translocation to the surface of endothelial cells is increased after exposure nitric oxide (NO) synthase inhibitor NG-nitro-L-arginine methyl ester (L-NAME), resulting in adhesiveness. L-NAME (3 mM) was added human cultured iliac vein for 1, 2, 4, and 6 h, mRNA expression quantified by a ribonuclease protection assay. In parallel experiments, NO donor, SPM-5185 (10 microM), venous cells, quantified. protein synthesis Western blot analysis. caused P-selection RNA at 2-4 whereas...
Abstract Objective. For the effective growth of malignant tumors, including glioblastoma, necessary factors involve endoplasmic reticulum (ER) stress, hypoxia, and availability nutrients, particularly glucose. The ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1) is involved in ER-associated (ERAD) targeting misfolded glycoproteins for an N-glycan-independent manner. EDEM1 was also identified as a new modulator insulin synthesis secretion. present study aims to investigate...
Abstract Objective. It is known that inhibition of the endoplasmic reticulum transmembrane signaling protein (ERN1) suppresses glioblastoma cells proliferation. The present study aims to investigate impact ERN1 endoribonuclease and kinase activities on TOB1 , HBEGF TWIST1 gene expression in U87MG with an intent reveal role regulation these genes. Methods. inhibited (dnrERN1) or both enzymatic (endoribonuclease kinase; dnERN1) were used. Cells transfected empty vector served as controls....
We hypothesize that poly (ADP-ribosyl)ation, is, (ADP-ribose) polymerase (PARP)-dependent transfer of ADP-ribose moieties from NAD to nuclear proteins, plays a role in diabetic nephropathy. evaluated whether PARP activation is present and two unrelated inhibitors, 3-aminobenzamide (ABA) 1,5-isoquinolinediol (ISO), counteract overexpression endothelin-1 (ET-1) ET receptors the renal cortex short-term diabetes. The studies were performed control rats streptozotocin-diabetic treated...
Previously we have shown that hypoxia strongly induces the expression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-3 and -4 (PFKFB-3 PFKFB-4) genes in several cancer cell lines via a HIF-dependent mechanism. In this paper studied hypoxic regulation PFKFB-4 PFKFB-3 mRNA as well its correlation with HIF-1alpha, HIF-2alpha, VEGF Glut1 pancreatic line Panc1 two gastric MKN45 NUGC3. This study clearly demonstrated are expresses MKN45, NUGC3 cancers cells both responsive to vitro....
Resuscitation from hemorrhage induces profound pathophysiologic alterations and activates inflammatory cascades able to initiate neutrophil accumulation in a variety of tissues. This process is accompanied by acute organ damage (e.g., lungs liver). We have previously demonstrated that significant leukocyte–endothelium interactions occur very early other forms ischemia/reperfusion (i.e., splanchnic traumatic shock) which are largely mediated increased expression the adhesion molecule,...
The aim of the present study was to examine association between expression insulin-like growth binding protein-1 and -2 (IGFBP1 IGFBP2), factor 2 mRNA protein 3/KH domain containing over-expressed in cancer (IGF2BP3/KOC1), HtrA serine peptidase 1/serine protease with IGF-binding (HTRA1/PRSS11) genes function endoplasmic reticulum stress signaling mediated by ERN1 (endoplasmic nucleus 1) as well regulation these hypoxia U87glioma cells.The IGFBP1, IGFBP2, IGF2BP3, HTRA1 U87 glioma cells its...
Abstract Objective. The aim of the present investigation was to study effect hypoxia on expression genes encoding endothelin-1 (EDN1) and its cognate receptors (EDNRA EDNRB) as well endothelin converting enzyme 1 (ECE1) in U87 glioma cells response inhibition endoplasmic reticulum stress signaling mediated by ERN1/IRE1 (endoplasmic nucleus 1) for evaluation their possible significance control growth through ERN1 hypoxia. Methods. level EDN1 , EDNRA EDNRB ECE1 micro-RNA miR-19, miR-96,...
The unique properties of single-walled carbon nanotubes (SWCNTs) make them viable candidates for versatile implementation in the biomedical devices. They are able to cross blood–brain barrier, enter cells and accumulate cell nuclei. We studied effect these nanoparticles on expression genes associated with endoplasmic reticulum stress proliferation, viability cancerogenesis as well microRNAs normal human astrocytes. have shown that treatment astrocytes by small doses SWCNTs (2 8 ng/ml medium...
Glucose and glutamine supply as well serine synthesis endoplasmic reticulum (ER) stress are important factors of glioblastoma growth. Previous studies showed that the knockdown ERN1 (ER to nucleus signaling 1) suppressed cell proliferation modified sensitivity numerous gene expressions nutrient deprivations. The present study is aimed investigate impact glucose deprivations on expression genes in U87MG cells relation with intent reveal role pathway ER stress-dependent regulation these...