A5083210524- Endoplasmic Reticulum Stress and Disease
- Cancer, Hypoxia, and Metabolism
- Genetics and Neurodevelopmental Disorders
- Autophagy in Disease and Therapy
- Pancreatic function and diabetes
- Adipose Tissue and Metabolism
- Mitochondrial Function and Pathology
- Adipokines, Inflammation, and Metabolic Diseases
- Graphene and Nanomaterials Applications
- Ubiquitin and proteasome pathways
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- Diet, Metabolism, and Disease
- Adenosine and Purinergic Signaling
- Galectins and Cancer Biology
- Cellular transport and secretion
- Advanced biosensing and bioanalysis techniques
- Genetics, Aging, and Longevity in Model Organisms
- Metabolism, Diabetes, and Cancer
- Nanoparticles: synthesis and applications
- Advanced Nanomaterials in Catalysis
- Hormonal Regulation and Hypertension
- Epigenetics and DNA Methylation
- Religious Education and Schools
- Nanoparticle-Based Drug Delivery
Palladin Institute of Biochemistry
2019-2025
National Academy of Sciences of Ukraine
2019-2025
Abstract Objective. For the effective growth of malignant tumors, including glioblastoma, necessary factors involve endoplasmic reticulum (ER) stress, hypoxia, and availability nutrients, particularly glucose. The ER degradation enhancing alpha-mannosidase like protein 1 (EDEM1) is involved in ER-associated (ERAD) targeting misfolded glycoproteins for an N-glycan-independent manner. EDEM1 was also identified as a new modulator insulin synthesis secretion. present study aims to investigate...
Abstract Objective. It is known that inhibition of the endoplasmic reticulum transmembrane signaling protein (ERN1) suppresses glioblastoma cells proliferation. The present study aims to investigate impact ERN1 endoribonuclease and kinase activities on TOB1 , HBEGF TWIST1 gene expression in U87MG with an intent reveal role regulation these genes. Methods. inhibited (dnrERN1) or both enzymatic (endoribonuclease kinase; dnERN1) were used. Cells transfected empty vector served as controls....
Abstract Objective. Endoplasmic reticulum (ER) stress and hypoxia are key factors for the effective growth of malignant tumors, including glioblastoma. The phosphoserine aminotransferase 1 (PSAT1) is an ER stress-responsive enzyme responsible serine synthesis necessary tumor cell proliferation. present study aims to investigate regulation PSAT1 gene expression in U87MG glioblastoma cells normal human astrocytes by depending on hydrocortisone, a native hormone used co-treatment other tumors....
Abstract Objective. The aim of the present investigation was to study effect hypoxia on expression genes encoding endothelin-1 (EDN1) and its cognate receptors (EDNRA EDNRB) as well endothelin converting enzyme 1 (ECE1) in U87 glioma cells response inhibition endoplasmic reticulum stress signaling mediated by ERN1/IRE1 (endoplasmic nucleus 1) for evaluation their possible significance control growth through ERN1 hypoxia. Methods. level EDN1 , EDNRA EDNRB ECE1 micro-RNA miR-19, miR-96,...
Glucose and glutamine supply as well serine synthesis endoplasmic reticulum (ER) stress are important factors of glioblastoma growth. Previous studies showed that the knockdown ERN1 (ER to nucleus signaling 1) suppressed cell proliferation modified sensitivity numerous gene expressions nutrient deprivations. The present study is aimed investigate impact glucose deprivations on expression genes in U87MG cells relation with intent reveal role pathway ER stress-dependent regulation these...
Abstract Objective. Serine synthesis as well endoplasmic reticulum stress and hypoxia are important factors of malignant tumor growth including glioblastoma. Previous studies have shown that the knockdown ERN1 (endoplasmic to nucleus signaling) significantly suppressed glioblastoma cell proliferation modified regulation. The present study is aimed investigate impact on expression PHGDH (phosphoglycerate dehydrogenase), PSAT1 (phosphoserine aminotransferase 1), PSPH phosphatase), ATF4...
Abstract Objective. Carboxypeptidase E (CPE) plays an important role in the biosynthesis of neurotransmitters and peptide hormones including insulin. It also promotes cell proliferation, survival, invasion tumor cells. The endoplasmic reticulum stress, hypoxia, nutrient supply are significant factors malignant growth glioblastoma. There data indicating that knockdown to nucleus signaling 1 (ERN1) suppressed glioblastoma proliferation increased invasiveness these present study aims...
Objective. Homeobox genes play a fundamental role in the embryogenesis, but some of them have been linked to oncogenesis. The present study is aimed investigate impact glucose and glutamine deprivations on expression homeobox such as PAX6 (paired box 6), PBX3 (PBX 3), PBXIP1 interacting protein 1), MEIS1 (MEIS MEIS2 ERN1 knockdown U87 glioma cells with intent reveal (endoplasmic reticulum nucleus signaling 1) pathway endoplasmic stress dependent regulation genes. Methods. control...
Objective. Single-walled carbon nanotubes (SWCNTs) are considered to be one of the nanomaterials attractive for biomedical applications, particularly in health sciences as imaging probes and drug carriers, especially field cancer therapy. The increasing exploitation necessitates a comprehensive evaluation potential impact these nanomaterials, which purposefully accumulate cell nucleus, on human function genome normal tumor tissues. aim this study was investigate sensitivity expression DNAJB9...
Abstract Objective. The aim of the present investigation was to study impact glucose and gluta-mine deprivations on expression genes encoding EDN1 (endothelin-1), its cognate receptors (EDNRA EDNRB), ECE1 (endothelin converting enzyme 1) in U87 glioma cells response knockdown ERN1 (endoplasmic reticulum nucleus signaling 1), a major pathway endoplasmic stress, for evaluation their possible implication control growth through nutrient limitations. Methods. level EDN1, 1 with treated by or...
Objective. Nanographene oxide, an oxidation derivative of graphene, is considered to be one the nanomaterials attractive for biomedical applications, although this nanomaterial toxic. The increasing exploitation graphene-based materials necessitates a comprehensive evaluation potential impact these on human health. Moreover, it necessary investigate in detail mechanisms its toxic effect living cells particularly at genome level. present study aimed evaluate low doses nanographene oxide...
Abstract Objective. Glucocorticoids are important stress-responsive regulators of insulin-dependent metabolic processes realized through specific changes in genome function. The aim this study was to investigate the impact cortisol on insulin receptor and related genes expression HEK293 cells upon induction endoplasmic reticulum (ER) stress by tunicamycin hypoxia. Methods. human embryonic kidney cell line used. Cells were exposed (10 µM) as well inducers hypoxia (dimethyloxalylglycine, DMOG;...
Homeobox genes play an important role in health and disease including oncogenesis. The present investigation aimed to study ERN1-dependent hypoxic regulation of the expression encoding homeobox proteins MEIS (zinc finger E-box binding 2) LIM 1 family, SPAG4 (sperm associated antigen 4) NKX3-1 (NK3 1) U87MG glioblastoma cells response inhibition ERN1 (endoplasmic reticulum nucleus signaling for evaluation their possible significance control growth.
Abstract Objective. Serine hydroxymethyltransferase (SHMT2) plays a multifunctional role in mitochondria (folate-dependent tRNA methylation, translation, and thymidylate synthesis). The endoplasmic reticulum stress, hypoxia, glucose glutamine supply are significant factors of malignant tumor growth including glioblastoma. Previous studies have shown that the knockdown to nucleus signaling 1 (ERN1) pathway stress strongly suppressed glioblastoma cell proliferation modified sensitivity these...
Nanographene oxide (nGO) nanoparticles (NPs) have unique properties and are widely used in various fields, including biomedicine. These NPs, however, also exhibit toxic ef-fects therefore, the understanding of molecular mechanism nGO toxicity is very im-portant mainly for nanomedicine, especially cancer therapy. This study aimed to examine impact NPs on expression genes associated with endoplasmic reticulum (ER) stress, proliferation, cancerogenesis both normal human astrocytes U87MG...
Abstract Objective. The aim of the present investigation was to study expression genes encoding homeobox proteins ZEB2 (zinc finger E-box binding 2), TGIF1 (TGFB induced factor 1), SPAG4 (sperm associated antigen 4), LHX1 (LIM LHX2, LHX6, NKX3-1 (NK3 and PRRX1 (paired related 1) in U87 glioma cells response glucose deprivation control with knockdown ERN1 (endoplasmic reticulum nucleus signaling major pathway endoplasmic stress signaling, for evaluation it possible significance growth through...
Abstract Objective. The aim of the present investigation was to study expression genes encoding IRS1 (insulin receptor substrate 1) and some other functionally active proteins in U87 glioma cells under silencing polyfunctional chaperone HSPB8 for evaluation possible significance this protein intergenic interactions. Methods. Silencing mRNA introduced by specific siRNA. level , HK2 GLO1 HOMER3 MYL9 NAMPT PER2 PERP GADD45A DEK studied quantitative polymerase chain reaction. Results. It shown...
The aim of this work was to study the association between expression glucose metabolism related genes and insulin resistance, which is changed in obese adolescents adult men with without for better understanding molecular basis development obesity complications evaluation possible contribution these resistance. Methods. level their regulations studied by real-time qPCR adipose tissue blood cells using SYBRGreen Mix specific each mRNA forward reverse primers. Total RNA extracted TRIZOL...