A5055533225- Endoplasmic Reticulum Stress and Disease
- Mitochondrial Function and Pathology
- Genetics and Neurodevelopmental Disorders
- Autophagy in Disease and Therapy
- Ubiquitin and proteasome pathways
- Cancer, Hypoxia, and Metabolism
- Pancreatic function and diabetes
- Growth Hormone and Insulin-like Growth Factors
- Nanoparticles: synthesis and applications
- Adipose Tissue and Metabolism
- RNA Interference and Gene Delivery
- CRISPR and Genetic Engineering
- RNA regulation and disease
- Graphene and Nanomaterials Applications
- Microtubule and mitosis dynamics
- Folate and B Vitamins Research
- Nuclear Structure and Function
- MicroRNA in disease regulation
- ATP Synthase and ATPases Research
- FOXO transcription factor regulation
- Hormonal Regulation and Hypertension
- Cancer, Lipids, and Metabolism
- Biochemical and Molecular Research
- Anesthesia and Neurotoxicity Research
- Sirtuins and Resveratrol in Medicine
Palladin Institute of Biochemistry
2014-2023
National Academy of Sciences of Ukraine
2014-2023
Abstract Objective. The aim of the present investigation was to study effect hypoxia on expression genes encoding endothelin-1 (EDN1) and its cognate receptors (EDNRA EDNRB) as well endothelin converting enzyme 1 (ECE1) in U87 glioma cells response inhibition endoplasmic reticulum stress signaling mediated by ERN1/IRE1 (endoplasmic nucleus 1) for evaluation their possible significance control growth through ERN1 hypoxia. Methods. level EDN1 , EDNRA EDNRB ECE1 micro-RNA miR-19, miR-96,...
The unique properties of single-walled carbon nanotubes (SWCNTs) make them viable candidates for versatile implementation in the biomedical devices. They are able to cross blood–brain barrier, enter cells and accumulate cell nuclei. We studied effect these nanoparticles on expression genes associated with endoplasmic reticulum stress proliferation, viability cancerogenesis as well microRNAs normal human astrocytes. have shown that treatment astrocytes by small doses SWCNTs (2 8 ng/ml medium...
Abstract Inhibition of ERN1/IRE1α (endoplasmic reticulum to nucleus signaling 1/inositol requiring enzyme-1α), the major pathway endoplasmic stress, significantly decreases tumor growth. We have studied expression transcription factors such as E2F8 (E2F factor 8), EPAS1 (endothelial PAS domain protein 1), TBX3 (T-box 3), ATF3 (activating FOXF1 (forkhead box F1), and HOXC6 (homeobox C6) in U87 glioma cells overexpressing dominant-negative defective endoribonuclease (dnr-ERN1) well both kinase...
Abstract Objective. The aim of the present investigation was to study expression genes encoding polyfunctional proteins insulinase (insulin degrading enzyme, IDE) and pitrilysin metallopeptidase 1 (PITRM1) in U87 glioma cells response inhibition endoplasmic reticulum stress signaling mediated by ERN1/IRE1 (endoplasmic nucleus 1) for evaluation their possible significance control metabolism through ERN1 as well hypoxia, glucose glutamine deprivations. Methods. level IDE PITRM1 studied...
Abstract Objective. The development of obesity and its metabolic complications is associated with dys-regulation various intrinsic mechanisms, which control basic processes via changes in the expression numerous regulatory genes. main goal this work was to study association between insulin-like growth factors (IGF1 IGF2) IGF-binding proteins insulin resistance obese adolescents for evaluation possible contribution these genes resistance. Methods. IGF1, IGF2, IGFBPs mRNA measured blood normal...
The aim of the present study was to investigate effect inhibition endoplasmic reticulum stress signaling mediated by IRE1/ERN1 (inositol-requiring enzyme 1/endoplasmic nucleus 1) on expression genes encoding different groups insulin-like growth binding proteins (IGFBP6 and IGFBP7) CCN family (IGFBP8/CTGF/CCN2, IGFBP9/NOV/CCN3, IGFBP10/CYR61/CCN1, WISP1/CCN4, WISP2/CCN5) its sensitivity glucose deprivation in U87 glioma cells.The IGFBP6, IGFBP7, IGFBP8, IGFBP9, IGFBP10, WISP1, WISP2 studied...
The aim of the present study was to examine effect glucose deprivation on expression genes encoded glucocorticoid receptor (NR3C1) and some related proteins (NR3C2, AHR, NRIP1, NNT, ARHGAP35, SGK1, SGK3) in U87 glioma cells response inhibition endoplasmic reticulum stress signaling mediated by ERN1/IRE1 (endoplasmic nucleus 1/inositol requiring enzyme 1) for evaluation their possible significance control growth through IRE1 deprivation.The NR3C1, NR3C2, SGK3 transfected empty vector pcDNA3.1...
Background: Endoplasmic reticulum to nucleus signaling 1 (ERN1) is a major pathway of endoplasmic stress and crucial for malignant tumor growth. Objective: The article aims discuss the recent progress in discovery targets their involvement Methods: Literature from PubMed database related growth chemoresistance was searched reviewed. Results: plays an important part involved invasion metastasis. Inhibition protein kinase endoribonuclease activities ERN1 significantly reduces through...
Abstract Objective. Serine synthesis as well endoplasmic reticulum stress and hypoxia are important factors of malignant tumor growth including glioblastoma. Previous studies have shown that the knockdown ERN1 (endoplasmic to nucleus signaling) significantly suppressed glioblastoma cell proliferation modified regulation. The present study is aimed investigate impact on expression PHGDH (phosphoglycerate dehydrogenase), PSAT1 (phosphoserine aminotransferase 1), PSPH phosphatase), ATF4...
Abstract Objective. The aim of the present investigation was to examine effect inhibition endoplasmic reticulum stress signaling, mediated by IRE1 (inositol requiring enzyme 1), which is a central mediator unfolded protein response on expression genes encoding glucocorticoid receptor (NR3C1) and some related proteins (SGK1, SGK3, NCOA1, NCOA2, ARHGAP35, NNT) their hypoxic regulation in U87 glioma cells for evaluation possible significance control growth. Methods. NR3C1,SGK1,SGK3, NNT cells,...
Objective. Single-walled carbon nanotubes (SWCNTs) are able to cross the blood-brain barrier, penetrate through cell membrane, and accumulate in nucleus, which purposefully allows their use health sciences as imaging probes drug carriers cancer therapy. The aim of this study was investigate effect low doses SWCNTs on expression microRNAs associated with proliferation brain development zebrafish (Danio rerio) embryos. Methods. embryos (72 h post fertilization) were exposed (2 8 ng/ml medium)...
Glycolysis and glutaminolysis as well endoplasmic reticulum stress are required for tumor progression suggests through regulation of the cell cycle. Inhibition ERN1/IRE1 (endoplasmic to nucleus signaling 1/inositol requiring enzyme 1), a central mediator stress, significantly suppresses glioma proliferation growth modifies sensitivity gene expressions glucose glutamine deprivation. We have studied expression genes encoded transcription factors such E2F8 (E2F factor 8), EPAS1 (endothelial PAS...
We have studied the effect of inhibition IRe1 (inositol requiring enzyme 1), which is a central mediator endoplasmic reticulum stress and controller cell proliferation tumor growth, on hypoxic regulation expression different related genes in U87 glioma cells.It was shown that hypoxia leads to up-regulation Il13Ra2, cD24, ING1, ING2, eNDOG, TRaPPc3, TSFM, MTIF2 at mRNa level control cells.Changes for ING1 CD24 were more significant.At same time, IRE1 modifies all genes.In particular, it...
The effect of single‐walled carbon nanotubes (SWCNTs) on the expression level several genes, related to cell cycle control, proliferation and migration in human glioma line U87 was investigated. exposure cells different concentrations during 24 48 h affects CCND2 (Cyclin D2), DTNA (Dystrobrevin alpha), PARVB (parvin beta), DUSP1 (dual specificity phosphatase 1), PFKFB3 (6‐phosphofructo‐2‐kinase/fructose‐2,6‐bisphosphatase‐3) PFKFB4 genes at mRNA level, as well alternative splice variants...
We investigated the impact of IRe1/eRN1 (inositol requiring enzyme 1/endoplasmic reticulum to nucleus signaling 1) knockdown on hypoxic regulation expression a subset proliferation and migration-related genes in U87 glioma cells.It was shown that hypoxia leads up-regulation MeST SNaI2, TcF8 GTF2F2 at mRNa level control cells.At same time did not affect TCF3 GTF2B transcription factor genes.In turn, inhibition IRE1 modified effect all studied genes, except MyBl1 GTF2B.For instance, IRe1...
Objective. Homeobox genes play a fundamental role in the embryogenesis, but some of them have been linked to oncogenesis. The present study is aimed investigate impact glucose and glutamine deprivations on expression homeobox such as PAX6 (paired box 6), PBX3 (PBX 3), PBXIP1 interacting protein 1), MEIS1 (MEIS MEIS2 ERN1 knockdown U87 glioma cells with intent reveal (endoplasmic reticulum nucleus signaling 1) pathway endoplasmic stress dependent regulation genes. Methods. control...
We have studied the expression of a subset genes encoding important tumor growth related factors in U87 glioma cells with IRE1 (inositol requiring enzyme-1) knockdown as well their hypoxic regulation. It was shown that levels activating transcription factor 6 (ATF6), clusterin (CLU), adhesion G protein-coupled receptor E5 (ADGRE5), transglutaminase 2, C polypeptide (TGM2), leukemia inhibitory (LIF), phosphoserine aminotransferase 1 (PSAT1), glyoxalase I (GLO1) and tetraspanin 13 (TSPAN13)...
Abstract Objective. The aim of the present investigation was to study impact glucose and gluta-mine deprivations on expression genes encoding EDN1 (endothelin-1), its cognate receptors (EDNRA EDNRB), ECE1 (endothelin converting enzyme 1) in U87 glioma cells response knockdown ERN1 (endoplasmic reticulum nucleus signaling 1), a major pathway endoplasmic stress, for evaluation their possible implication control growth through nutrient limitations. Methods. level EDN1, 1 with treated by or...
Activation of cell proliferation and surviving as well an increased angiogenesis are important for tumor growth through signaling pathways the unfolding protein response/endoplasmic reticulum stress, which is a fundamental phenomenon secure protection cells by maintaining functional integrity endoplasmic reticulum. The response aims to resolve stress expanding protein-folding apparatus, decreasing load newly synthesized proteins, enhancing degradation removal improperly folded proteins from...
The aim of the present study was to examine effect chromium disilicide and titanium nitride nanoparticles on expression level genes encoding important regulatory factors (IGFBP1, IGFBP2, IGFBP3, IGFBP4, IGFBP5, SNARK/NUAK2, CD36, PECAM1/CD31) in mouse liver for evaluation possible toxic effects these nanoparticles.Male mice received 20 mg (45 nm) (20 with food every working day 2 months. IGFBP1, SNARK, PECAM1 studied by quantitative polymerase chain reaction.Treatment led down-regulation...
Abstract IRE-1α (inositol requiring enzyme-1α), the most evolutionarily conserved of endoplasmic reticulum stress signaling pathways, is highly implicated in sustaining proliferation glioma cells and subsequent tumor growth, which decreased by inhibition IRE-1α. To explore mediated regulation ubiquitin system cells, expression a subset specific peptidases (USP) activating enzyme E1-like protein/autophagy related 7 (GSA7/ATG7) genes was studied, during hypoxic wild type U87 with inhibited...