A5078492380- Influenza Virus Research Studies
- Animal Virus Infections Studies
- Animal Disease Management and Epidemiology
- Viral gastroenteritis research and epidemiology
- Virology and Viral Diseases
- Respiratory viral infections research
- Immune Response and Inflammation
- Virus-based gene therapy research
- Viral Infections and Immunology Research
- Viral Infections and Vectors
- interferon and immune responses
- Microbial infections and disease research
- Vector-Borne Animal Diseases
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Parvovirus B19 Infection Studies
- Herpesvirus Infections and Treatments
- Bacteriophages and microbial interactions
- Streptococcal Infections and Treatments
- Oral microbiology and periodontitis research
- Escherichia coli research studies
- Salmonella and Campylobacter epidemiology
- Plant Virus Research Studies
- Livestock and Poultry Management
- Digital Imaging for Blood Diseases
U.S. National Poultry Research Center
2015-2025
Agricultural Research Service
2015-2025
United States Department of Agriculture
2008-2025
Government of the United States of America
2023
University of Georgia
2003-2009
Poultry Research Institute
2002
Morehouse School of Medicine
1992
Emory University
1991-1992
Centers for Disease Control and Prevention
1991
National Center for Infectious Diseases
1990
ABSTRACT A real-time reverse-transcription PCR (RRT-PCR) was developed to detect avian paramyxovirus 1 (APMV-1) RNA, also referred as Newcastle disease virus (NDV), in clinical samples from birds. The assay uses a single-tube protocol with fluorogenic hydrolysis probes. Oligonucleotide primers and probes were designed sequences conserved region of the matrix protein (M) gene that recognized diverse set ( n = 44) APMV-1 isolates. second primer-probe targeted fusion (F) code for cleavage site...
The highly pathogenic avian influenza (HPAI) H5N1 virus clade 2.3.4.4b has caused the death of millions domestic birds and thousands wild in USA since January 2022 (refs.
Different genotypes of avian paramyxovirus serotype-1 virus (APMV-1) circulate in many parts the world. Traditionally, Newcastle disease (NDV) is recognized as having two major divisions represented by classes I and II, with class II being further divided into sixteen genotypes. Although all NDV are members APMV-1 one serotype, antigenic genetic diversity observed between different Reports vaccine failure from countries reports our lab on reduced ability classical vaccines to significantly...
We describe here the development of a mouse subcutaneous chamber model that allows for examination host-parasite interactions as well determination gross pathology with Porphyromonas (Bacteroides) gingivalis challenge. When inoculated into stainless-steel chambers implanted subcutaneously in female BALB/c mice, P. W83, W50, and A7436 (10(8) to 10(10) CFU) caused cachexia, ruffling, general erythema phlegmonous, ulcerated, necrotic lesions, death. W50/BEI, HG405, 33277 (10(10) produced...
Current technologies with next generation sequencing have revolutionized metagenomics analysis of clinical samples. To achieve the non-selective amplification and recovery low abundance genetic sequences, a simplified Sequence-Independent, Single-Primer Amplification (SISPA) technique in combination MiSeq platform was applied to target negative- positive-sense single-stranded RNA viral sequences. This method allowed successful sequence assembly full or near length avian influenza virus...
Low pathogenicity avian influenza virus (LPAIV) and lentogenic Newcastle disease (l NDV) are commonly reported causes of respiratory in poultry worldwide with similar clinical pathobiological presentation. Co-infections do occur but not easily detected, the impact co-infections on pathobiology is unknown. In this study chickens turkeys were infected a l NDV vaccine strain (LaSota) H7N2 LPAIV (A/turkey/VA/SEP-67/2002) simultaneously or sequentially three days apart. No signs observed...
Newcastle disease virus (NDV) is an avian paramyxovirus that causes significant economic losses to the poultry industry worldwide. There limited knowledge about immune response infection with virulent NDVs, and how this may contribute disease. In study, pathogenesis transcriptional host of chickens a NDV strain rapidly 100 % mortality was characterized. Using microarrays, strong observed in spleens at early times after induction groups genes involved innate antiviral pro-inflammatory...
Wild aquatic birds have been associated with the intercontinental spread of H5 subtype highly pathogenic avian influenza (HPAI) viruses A/goose/Guangdong/1/96 (Gs/GD) lineage during 2005, 2010, and 2014, but dispersion by wild waterfowl has not implicated other HPAI viruses. To better understand why Gs/GD infect transmit more efficiently in than viruses, groups mallard ducks were challenged one 14 different H7 including a H5N1 (clade 2.2) virus from Mongolia, part 2005 dispersion, H5N8 H5N2...
Infections with the highly pathogenic avian influenza (HPAI) H5N1 clade 2.3.4.4b virus have resulted in death of millions domestic birds and thousands wild U.S. since January, 2022. Throughout this outbreak, spillovers to mammals been frequently documented. Here, we report detection HPAI dairy cattle herds across several states The affected cows displayed clinical signs encompassing decreased feed intake, altered fecal consistency, respiratory distress, milk production abnormal milk....
Serum bactericidal activity (SBA) and ELISA antibody levels elicited by two efficacious serogroup B meningococcal vaccines were measured in a controlled trial involving 408 15- to 20-year-olds. Subjects given doses at 6-week interval of or control vaccine. Response was defined as > = 4-fold rise level. After the Finlay Institute (Havana) vaccine 12 months, proportions SBA responders not different from those group (15% 17% [vaccine] vs. 13% 9% [control], P .05). National Public Health (Oslo)...
During the spring of 2002, a low pathogenic avian influenza (LPAI) A (H7N2) virus caused major outbreak among commercial poultry in Virginia and adjacent states. The primarily affected turkey flocks, causing respiratory distress decreased egg production. Experimentally, turkeys were more susceptible than chickens to H7N2 infection, with 50% bird infectious dose titers equal 100.8 102.8–3.2, respectively. Comparison shedding from cloaca oropharynx demonstrated that recent isolates readily...
Exotic Newcastle disease virus (NDV) isolated from chickens during the 2002-2003 California outbreak (CA exotic [END] virus) was inoculated into 4-week-old specificpathogen-free (SPF) White Leghorn chickens, 3-week-old SPF Beltsville turkeys, 6-week-old commercial Broad Breasted and 10- to 20-week-old racing pigeons, clinicopathologic features of were compared. Birds monitored clinically euthanized sequentially with collection tissues. Tissues examined by histopathology, immunohistochemistry...
Vaccination is an important tool in the protection of poultry against avian influenza (AI). For field use, overwhelming majority AI vaccines produced are inactivated whole virus formulated into oil emulsion. However, recombinant vectored gaining use for their ability to induce heterologous isolates and overcome maternal antibody interference. In these studies, we compared chickens provided by a turkey herpesvirus (HVT) vector vaccine expressing hemagglutinin (HA) gene from clade 2.2 H5N1...
ABSTRACT In June of 2012, an H7N3 highly pathogenic avian influenza (HPAI) virus was identified as the cause a severe disease outbreak in commercial laying chicken farms Mexico. The purpose this study to characterize Mexican 2012 HPAI (A/chicken/Jalisco/CPA1/2012) and determine protection against conferred by different H7 inactivated vaccines chickens. Both adult young chickens intranasally inoculated with became infected died at between 2 4 days postinoculation (p.i.). High titers viral...
The virulence determinants for highly pathogenic avian influenza viruses (AIVs) are considered multigenic, although the best characterized factor is hemagglutinin (HA) cleavage site. capability of to reassort gene segments one potential way new emerge with different characteristics. To evaluate role other in virulence, we used reverse genetics generate two H5N1 recombinant differing pathogenicity chickens. Single-gene reassortants were determine which viral genes contribute altered...
From December 2014 through June 2015, the US experienced most costly highly pathogenic avian influenza (HPAI) outbreak to date. Most cases in commercial poultry were caused by an H5N2 strain which was a reassortant with 5 Eurasian lineage genes, including clade 2.3.4.4 goose/Guangdong/1996 hemagglutinin, and 3 genes from North American wild waterfowl low pathogenicity viruses. The primarily affected turkeys table-egg layer type chickens. Three isolates selected for characterization turkeys:...
Maternally-derived antibodies (MDA) provide early protection from disease, but may interfere with active immunity in young chicks. In highly pathogenic avian influenza virus (HPAIV)-enzootic countries, broiler chickens typically have MDA to Newcastle disease (NDV) and H5 HPAIV, their impact on recombinant vectored vaccines is unclear. We assessed the effectiveness of a spray-applied NDV vaccine AIV insert (rNDV-H5) turkey herpesvirus (HVT) (rHVT-H5) commercial broilers alone (MDA:AIV−NDV+)...
Avian metapneumovirus (aMPV) poses a significant threat to the poultry industry worldwide, primarily affecting turkeys and chickens. The recent detection of aMPV-A -B subtypes in United States marks shift after prolonged period free aMPV following eradication previously circulating subtype C. Hence, demand for molecular diagnostic tests has arisen due their limited availability US market. In this study, we present characterization based on complete genome sequence A, which was detected first...
A whole-genome shotgun sequencing (sWGS) approach was applied to chicken clinical tracheal swab samples during metagenomics investigations identify possible microorganisms among poultry with respiratory diseases. After applying sequencing, Ornithobacterium rhinotracheale (ORT) and a putative prophage candidate were found in one of the samples. multi-locus sequence typing (MLST) scheme ORT genome involved adk, aroE, fumC, gdhA, pgi, pmi genes. Antibiotic resistant analysis demonstrated...