A5078150047- Urological Disorders and Treatments
- Tissue Engineering and Regenerative Medicine
- Urinary Bladder and Prostate Research
- Bladder and Urothelial Cancer Treatments
- Epigenetics and DNA Methylation
- Viral Infections and Immunology Research
- Connective tissue disorders research
- Urinary Tract Infections Management
- Pelvic floor disorders treatments
- Aldose Reductase and Taurine
- Spectroscopy Techniques in Biomedical and Chemical Research
- Cell Adhesion Molecules Research
- Urologic and reproductive health conditions
- Pediatric Urology and Nephrology Studies
- Neuroblastoma Research and Treatments
- Cardiac Fibrosis and Remodeling
- Circadian rhythm and melatonin
- T-cell and B-cell Immunology
- Cytokine Signaling Pathways and Interactions
- Protease and Inhibitor Mechanisms
- Electrospun Nanofibers in Biomedical Applications
- Muscle Physiology and Disorders
- Hernia repair and management
- Hippo pathway signaling and YAP/TAZ
- Virus-based gene therapy research
Hospital for Sick Children
2013-2024
St. Clair College
2024
University of Toronto
2001-2023
Biologie du Développement et Cellules Souches
2017-2018
SickKids Foundation
2001-2017
Great Ormond Street Hospital
1992-2017
University College London
1992-2017
Technion – Israel Institute of Technology
2007
Mansoura University
2005
University Health Network
2000
Abstract —Viral myocarditis is an important cause of heart failure and dilated cardiomyopathy. T lymphocytes are implicated in myocardial damage murine models coxsackievirus B3 (CVB3) myocarditis. We used knockout mice lacking CD4 (CD4 −/− ), CD8 (CD8 both coreceptors or the T-cell receptor β chain (TCRβ ) to address contribution subpopulations host susceptibility CVB3 Severity disease was magnified but attenuated mice, consistent with a pathogenic role for + lymphocytes. Elimination...
Bladder exstrophy epispadias complex (BEEC) is a severe congenital anomaly; however, the genetic and molecular mechanisms underlying formation of BEEC remain unclear. TP63, member TP53 tumor suppressor gene family, expressed in bladder urothelium skin over external genitalia during mammalian development. It plays role We have previously shown that p63(-/-) mouse embryos developed phenotype identical to human BEEC. hypothesised TP63 involved pathogenesis. RNA was extracted from foreskin...
Neuroblastoma (NB), a tumor of the primitive neural crest, despite aggressive treatment portends poor long-term survival for patients with advanced high stage NB. New strategies are required.We investigated coordinated targeting essential homeostatic regulatory factors involved in cancer progression, histone deacetylases (HDACs) and carbonic anhydrases (CAs).We evaluated antitumor potential HDAC inhibitor (HDACi), pyridylmethyl-N-{4-[(2-aminophenyl)-carbamoyl]-benzyl}-carbamate (MS-275)...
Extracellular matrix changes are often crucial inciting events for fibroproliferative disease. Epigenetic changes, specifically DNA methylation, critical factors underlying differentiated phenotypes. We examined the dependency of matrix-induced fibroproliferation and SMC phenotype on methyltransferases. The cooperativity with growth factors, cell density hypoxia was also examined. Primary rat visceral early passage (0-2) were plated native collagen or damaged/heat-denatured collagen. Hypoxia...
Partial bladder outlet obstruction causes prominent morphological changes in the wall, which leads to dysfunction. In this paper, we demonstrate that polarized light imaging can be used identify location of induced structural other modalities fail detect. We 2-week and 6-week partial rats, harvested obstructed bladders, then measured their retardances while distended high pressures compared them controls. Our results show retardance central part ventral side (above ureters) closer urethra as...
Congenital bladder outlet obstruction from either mechanical or functional causes often results in clinical fibrosis. We tested the hypothesis that early molecular changes relevant to fibrosis occur response stretch injury of wall and specific extracellular matrix receptors mediate some these responses. Furthermore, we introduce a novel ex vivo model which has advantages over previously described models by uniquely interrogating responses distention.The bladders Sprague Dawley rats were...
Previous molecular studies showed that the mTOR inhibitor rapamycin prevents bladder smooth muscle hypertrophy in vitro. We investigated effect of treatment vivo on a rat model partial outlet obstruction.A total 48 female Sprague-Dawley® rats underwent obstruction and received daily subcutaneous injections (1 mg/kg) or vehicle commencing 2 weeks postoperatively. A 36 sham surgery vehicle. Rats were sacrificed 3, 6 12 after surgery. Before sacrifice, voiding was observed metabolic cage for 24...
No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Nov 2003Collagen Directly Stimulates Bladder Smooth Muscle Cell Growth In Vitro: Regulation by Extracellular Regulated Mitogen Activated Protein Kinase DANIEL B. HERZ, KAREN AITKEN, and DARIUS J. BAGLI HERZDANIEL HERZ Current address: Pediatric Urology Associates, P. C., Medical Arts Atrium, Westchester Center, 19 Bradhurst Ave., Suite 2575, Hawthorne, New York 10532. More articles this author , AITKENKAREN AITKEN BAGLIDARIUS View All Author...
It is unknown how bladder smooth muscle cells sense extrinsic mechanical stimuli. The integrins are a large versatile family of transmembrane mechanoreceptors that transduce extracellular matrix (ECM) alterations into the cell, thereby, regulating proliferation, differentiation and ECM synthesis. To our knowledge we provide first evidence may be involved in responses to whole distention cell stretch.Bladders from 100 120 gm. rats were stretched 40 cm. H2O for 5 minutes. Five 96 hours after...
Microstructural remodelling in epithelial layers of various hollow organs, including changes tissue anisotropy, are known to occur under mechanical distension and during disease processes. In this paper, we analyze how bladder alters wall anisotropy using polarized light imaging (followed by Mueller matrix decomposition). Optical retardance values different regions normal rat bladders pressures derived. Then optical coherence tomography is used measure local thicknesses, enabling the...
Mammalian circadian control is determined by a central clock in the brain suprachiasmatic nucleus (SCN) and synchronized peripheral clocks other tissues. Increasing evidence suggests that SCN-independent regulation of also occurs. We examined how activation excitatory receptors influences protein PERIOD 2 (PER2) contractile organ, urinary bladder. PERIOD2:: LUCIFERASE-knock-in mice were used to report realtime PER2 dynamics bladder tissue. Rhythmic activities occurred wall with cycle ~24 h...
Host-pathogen interactions can induce epigenetic changes in the host directly, as well indirectly through secreted factors. Previously, uropathogenic Escherichia coli (UPEC) was shown to increase DNA methyltransferase activity and expression, which associated with methylation-dependent alterations urothelial expression of CDKN2A. Here, we showed that paracrine factors from infected cells alter another writer, EZH2, coordinate proliferation. Urothelial were inoculated UPEC, UPEC derivatives,...
No AccessJournal of UrologyINVESTIGATIVE UROLOGY1 Feb 2003Integrins Expressed With Bladder Extracellular Matrix After Stretch Injury In Vivo Mediate Smooth Muscle Cell Growth Vitro JYOTI UPADHYAY, KAREN J. AITKEN, CHRISTOPHER DAMDAR, STEPHANE BOLDUC, and DARIUS BÄGLI UPADHYAYJYOTI UPADHYAY More articles by this author , AITKENKAREN AITKEN DAMDARCHRISTOPHER DAMDAR BOLDUCSTEPHANE BOLDUC BÄGLIDARIUS View All Author Informationhttps://doi.org/10.1016/S0022-5347(05)64007-4AboutFull TextPDF...
We developed a cell transfer technology for covering demucosalized colonic segments with bladder urothelium. This would be achieved through aerosol spraying of single suspension urothelial and smooth muscle cells fibrin glue onto the segments.In 6 piglets (20 kg.) 4 cm.2 area was excised. Single prepared. A segment detubularized sigmoid colon isolated on its vascular pedicle demucosalized. The suspensions were combined an equal volume sprayed over raw submucosal surface segment....