A5001955204- Angiogenesis and VEGF in Cancer
- Platelet Disorders and Treatments
- Protease and Inhibitor Mechanisms
- Blood properties and coagulation
- TGF-β signaling in diseases
- Cancer-related molecular mechanisms research
- Kruppel-like factors research
- Antiplatelet Therapy and Cardiovascular Diseases
- Congenital heart defects research
- MicroRNA in disease regulation
- Connective Tissue Growth Factor Research
- Circular RNAs in diseases
- Developmental Biology and Gene Regulation
- ATP Synthase and ATPases Research
- Zebrafish Biomedical Research Applications
- Complement system in diseases
- Fibroblast Growth Factor Research
- Myeloproliferative Neoplasms: Diagnosis and Treatment
- Infectious Aortic and Vascular Conditions
- Calpain Protease Function and Regulation
- Protein Hydrolysis and Bioactive Peptides
- Multiple Myeloma Research and Treatments
- Bone Metabolism and Diseases
- Inflammatory Biomarkers in Disease Prognosis
- Mechanical Circulatory Support Devices
University of Freiburg
2014-2024
Universitäts-Herzzentrum Freiburg-Bad Krozingen
2014-2024
University Medical Center Utrecht
2015
University Medical Center Freiburg
2015
NewYork–Presbyterian Hospital
2014
Cornell University
2014
University of North Carolina at Chapel Hill
2014
New York Hospital Queens
2014
Presbyterian Hospital
2014
Heinrich Heine University Düsseldorf
1990-1991
Background— Adaptive neovascularization after arterial occlusion is an important compensatory mechanism in cardiovascular disease and includes both the remodeling of pre-existing vessels to collateral arteries (arteriogenesis) angiogenic capillary growth. We now aimed identify regulatory microRNAs involved modulation femoral artery mice. Methods Results— Using microRNA-transcriptome analysis, we identified miR-155 as a downregulated microRNA during hindlimb ischemia. Correspondingly,...
Summary MicroRNAs are small non-coding RNAs that negatively regulate posttranscriptional gene expression. Several microRNAs have been described to the process of angiogenesis. Previously, we shown bone morphogenetic protein 4 (BMP4) increased proangiogenic activity endothelial cells. In this project, now investigated how pro-angiogenic BMP4 effect is mediated by microRNAs. First, performed a microRNA array with BMP4-stimulated human umbilical vein cells (HUVECs). Among topregulated...
Abstract Occlusive vascular diseases are promoted by a “prethrombotic state” with increased platelet activity. Polymerization of cytoskeletal proteins and exposure subcellular structures or rebinding secreted have been characterized as early reactions after activation preceding adhesion aggregation. Here, we demonstrate the kinetic increase in specific binding monoclonal antibodies to thrombospondin (P10) membrane markers CD63 (GP53, 53 kD lysosomal protein) CD62 (GMP140, 140 alpha granule...
Objective— Previously, we have identified bone morphogenetic protein endothelial cell precursor–derived regulator (BMPER) to increase the angiogenic activity of cells in a concentration-dependent manner. In this project, now investigate how BMPER acts concert with key molecules angiogenesis promote blood vessel formation. Approach and Results— To assess effect on angiogenesis-related signaling pathways, performed an antibody array BMPER-stimulated cells. We detected increased basic...
Simultaneous generation of neural cells and that the nutrient-supplying vasculature during brain development is called neurovascular coupling. We report on a transgenic mouse with impaired transforming growth factor β (TGFβ)-signalling in forebrain-derived using Foxg1-cre knock-in to drive conditional knock-out Tgfbr2. Although expression FOXG1 assigned progenitors neurons telencephalon, Foxg1(cre/+);Tgfbr2(flox/flox) (Tgfbr2-cKO) mutants displayed intracerebral haemorrhage. Blood vessels...
Aldosterone is a key factor in adverse cardiovascular remodeling by acting on the mineralocorticoid receptor (MR) different cell types. Endothelial MR activation mediates hypertrophy, inflammation and fibrosis. Cardiovascular often accompanied impaired angiogenesis, which risk for development of heart failure. In this study, we evaluated impact endothelial cells angiogenesis. Deoxycorticosterone acetate (DOCA)-induced hypertension was associated with capillary rarefaction WT mice but not...
Abstract This study was intended to investigate the actual platelet activation status after an acute coronary event. The of circulating platelets assayed directly by measuring membrane markers CD62 and CD63 with Düssel‐dorf III flow cytometry test in 22 patients diagnosis myocardial infarction during 48‐h observation period following number activated, marker‐positive sample significantly increased post‐MI patients: CD62: 5·8%× 2·25 ±1 vs. 3·5%× 2·32 , P ≤ 0·05; CD63: 18·7%× 1·77 4·6%× 2·16...
Myocardial infarction (MI) is a major cause of heart failure. The carboxypeptidase cathepsin A novel target in the treatment cardiac We aim to show that recently developed inhibitors protease attenuate post-MI Mice were subjected permanent left anterior descending artery (LAD) ligation or sham operation. 24 h post–surgery, LAD-ligated animals treated with daily doses inhibitor SAR1 placebo. After 4 weeks, three groups (sham, MI-placebo, MI-SAR1) evaluated. Compared sham-operated animals,...
Proper blood vessel formation is essential in health and often dysregulated ischaemic diseases. Therefore, regulatory mechanisms that control angiogenesis arteriogenesis are required to improve treatment of The aim this study was investigate the role homeobox transcription factor HoxB5 overexpression during revascularization disease. To assess effect on vivo, we subjected C57BL/6 mice femoral artery ligation with local intramuscular injection or green fluorescent protein adenoviral vectors....
The bone morphogenetic protein (BMP) signaling pathway plays a central role during vasculature development. Mutations or dysregulation of the BMP members have been linked to arteriovenous malformations. In present study, we investigated effect modulators endothelial precursor-derived regulator (BMPER) and twisted gastrulation homolog 1 (TWSG1) on specification zebrafish development analyzed downstream Notch in human cells. Silencing bmper twsg1b embryos by morpholinos resulted pronounced...
Regarding branching morphogenesis, neurogenesis and angiogenesis share common principle mechanisms make use of the same molecules. Therefore, investigation neuronal molecules involved in vascular morphogenesis provides new possibilities for pro-angiogenic approaches cardiovascular diseases.In this study, we investigated role transcription factor NPAS4 angiogenesis.Here, demonstrate that is expressed endothelial cells different origin using reverse PCR western blot analysis. To investigate...
The purpose of this study is to investigate the role platelet bone morphogenetic proteins (BMP)-4 during vascular inflammation and remodeling in a mouse model carotid wire injury. Transgenic mice with platelet-specific deletion BMP-4 (
'Ex vivo' testing of functional platelet properties using conventional techniques reflects the overall behaviour whole population in sample under investigation. Since many aspects depend on ultrastructural constituents which may vary from one cell to another, multiparameter single analysis platelets be advantageous providing direct insight into deviations at cellular level relevant pathophysiology disease states such as bleeding disorders or thrombophilia. Immunolabelling with monoclonal...
Dedifferentiated vascular smooth muscle cells (vSMCs) play an essential role in neointima formation, and we now aim to investigate the of bone morphogenetic protein (BMP) modulator BMPER (BMP endothelial cell precursor-derived regulator) formation. To assess expression arterial restenosis, used a mouse carotid ligation model with perivascular cuff placement. Overall after vessel injury was increased; however, tunica media decreased compared untreated control. Consistently, proliferative,...
Background: Veno-arterial extracorporeal membrane oxygenation (VA-ECMO) is used for critically ill patients requiring hemodynamic support but has been shown to induce an inflammatory response syndrome potentially leading severe complications and poor outcome. Monocytes are comprised of different subsets play a central role in the innate immune system. The unique small binding proteins, Designed Ankyrin Repeat Protein “F7” single chain variable fragment “MAN-1,” specifically detect activated...
Vascular smooth muscle cells (VSMCs) play a key role in aortic aneurysm formation. Bone morphogenetic proteins (BMPs) have been implicated as important regulators of VSMC phenotype, and dysregulation the BMP pathway has shown to be associated with vascular diseases. The aim this study was investigate for first time effects BMP-4 on phenotype understand its development thoracic aneurysms (TAAs). Using angiotensin II (AngII) osmotic pump model mice, aortas from mice VSMC-specific deficiency...
Abstract Background C-type natriuretic peptide (CNP) is an endothelium-derived paracrine molecule with important role in vascular homeostasis. In septic patients, the serum level of amino-terminal propeptide CNP (NT-proCNP) shows a strong positive correlation inflammatory biomarkers and, if elevated, correlates disease severity and indicates poor outcome. It not yet known whether NT-proCNP also clinical outcome patients suffering from severe acute respiratory syndrome coronavirus 2...