This study was conducted to investigate the extent of platelet-leukocyte adhesion and platelet, monocyte, neutrophil activation in septic patients analyze whether these variables correlate with severity sepsis. Forty-seven consecutively admitted operative ICU a University Medical Centre 12 control prior elective surgery were included this prospective cohort study. Patients evaluated daily for sepsis criteria sepsis-associated organ failure assessment (SOFA) score used describe failure....

Abstract It has been suggested that extracts of feverfew may inhibit platelet behaviour via effects on sulphydryl groups. In the present study we have obtained evidence for such a mode action. (i) Compounds contain groups as cysteine and N-(2-mercaptopropionyl)glycine prevented inhibition by feverfew. (ii) Feverfew parthenolide (one active components feverfew) dramatically reduced number acid-soluble in platelets. This effect occurred at concentrations similar to those inhibited secretory...

The adhesion of activated platelets to leukocytes (rosette formation) seems be mediated by CD62 on and its counter-receptor (CD15 or a sialic acid-containing glycoprotein) polymorphonuclear (PMNL). However, neither treatment with an anti-CD62 antibody fucoidan nor PMNL anti-CD15 neuraminidase are able inhibit completely the adhesion. Therefore, we have studied platelet GPIIb/IIIa complex (CD41a) for involvement in PMNL. following evidences point participation CD41a leukocytes: a) inhibition...

Objective To determine the degree of regional and systemic coagulation activation soon after isolated severe head injury. Design Prospective, controlled clinical study. Setting The emergency room intensive care unit (ICU) a trauma center in university hospital serving population 5 million people. Patients Twenty-four victims: 20 patients with injury (head group, Glasgow Coma Score <or=to8) four bone fracture (control group). Interventions Insertion central venous, i.e. superior caval vein,...

Abstract Occlusive vascular diseases are promoted by a “prethrombotic state” with increased platelet activity. Polymerization of cytoskeletal proteins and exposure subcellular structures or rebinding secreted have been characterized as early reactions after activation preceding adhesion aggregation. Here, we demonstrate the kinetic increase in specific binding monoclonal antibodies to thrombospondin (P10) membrane markers CD63 (GP53, 53 kD lysosomal protein) CD62 (GMP140, 140 alpha granule...

Disintegrins represent a group of disulfide-rich peptides ranging in size from 41 to over 80 residues and are antagonists several integrin receptors. containing an RGD or KGD sequence potent inhibitors platelet aggregation as they block the binding fibrinogen αIIbβ3 integrin. The high affinity comparison short linear has been attributed localisation within defined three-dimensional structure. Cystine knot microproteins members another family small that consist only 28–40 amino acid residues....

Imaging of cerebral vein thrombosis is still challenging. Currently, diagnosis based on CT venography and MRI including MRA conventional digital subtraction angiography. However, especially in chronic cases, each method has shown its limitations. Newer strategies for are found molecular imaging using targeted contrast agents. The aim this study was to prove the feasibility a novel fibrin-targeted MR agent (EP-2104R; EPIX Pharmaceuticals) selective sinus venous an animal model.Thrombosis...

Platelet activation is accompanied by changes in the composition of platelet cytoskeleton with rapid incorporation and displacement certain proteins. Here we have inhibited cytoskeletal assembly pretreating platelets cytochalasin D (CyD) investigated effect on stability aggregates that form. The experiments were performed both citrated hirudinized platelet-rich plasma (PRP) aggregation was induced adenosine diphosphate (ADP), collagen, TXA2-mimetic U46619 adrenaline. Platelets formed,...

Electrical peripheral nerve neurostimulation (PNS) is reported to be an effective pain treatment. An objective proof of antinociceptive effect lacking. The human experimental study addressed PNS effects on nociception and by electrophysiology psychophysics. In 23 healthy volunteers, 39 sessions were conducted. Three experiments (PNS ipsilateral, contralateral, Control) consisted 13 each. Conditioning (100 Hz) left ipsilateral) or right contralateral) superficial radial trunk evoked...

The purpose of this study was to describe the usefulness recent advances neuronavigational technology in management skull base tumors and vascular lesions, treated via a approach.In 16 patients (skull meningioma n = 9, petrous apex epidermoid l, craniopharyngeoma 1, giant internal carotid artery aneurysm basilar/vertebral 2, brain stem cavernoma 2), "advanced" neuronavigation used. In contrast "conventional" neuronavigation, information for neurosurgeon enhanced by intraoperative screen...

<b>Introduction</b> Antithrombotic medication has proven efficacy in the treatment and prevention of cardiovascular cerebrovascular diseases. A major disadvantage is increased incidence cerebral hemorrhages such as subdural hematomas (SDH). The impact antithrombotic therapy on outcome hematoma not well characterized to date. <b>Materials Methods</b> We retrospectively analyzed patients with admitted our hospital between 2005 2009. medication, other risk factors (age, sex, preexisting...

Summary A patient with idiopathic thrombocytopenic purpura developed after splenectomy a thrombasthenia-like severe haemor-rhagic diathesis characterized by normal or subnormal platelet count, prolonged bleeding time, strongly reduced adhesion to glass and defective aggregation in response ADP collagen. In contrast hereditary thrombasthenia membrane glycoproteins (GP) lib Ilia were normally present the patient’s platelets. Immunoelectrophoretic analysis revealed an abnormal behaviour of GP...

The degradation of platelet-activating factor (PAF) and lipid concentrations were measured in sera from 20 patients with insulin-dependent diabetes mellitus age- sex-matched healthy volunteers. PAF-degrading capacity as well triglycerides total very low density low-density lipoprotein cholesterol found to be significantly increased the group, whereas difference observed high-density was statistically nonsignificant. There also a series close relationships between PAF some variables control...

AbstractThe purpose of this investigation was to obtain information on platelet-leukocyte conjugate formation in whole blood and factors that affect it. We also measured platelet leukocyte activation by quantitating the expression CD62P CD11b. In both cases a flow cytometric approach used. The results show platelet-monocyte platelet-polymorphonuclear (PMNL) is enhanced simply stirring blood, with optimum occurring after 10 min. case monocytes, adenosine diphosphate (ADP). Both monocyte PMNL...

AbstractPlatelets in stirred whole blood can be induced to form aggregates and also heterotypic platelet-monocyte (P/M) platelet-neutrophil (P/N) conjugates. Here we have investigated the effects of three GPIIb-IIIa antagonists (GR144053F, MK-852 ReoproTM, a CD62P-blocking antibody, GA6, EDTA on conjugate formation that occurs stirring response adding ADP PAF. We confirmed identities conjugates by light microscopy after cell sorting. Platelet aggregation was measured platelet counting....