A5061848625- Monoclonal and Polyclonal Antibodies Research
- Biochemical and Structural Characterization
- Protein purification and stability
- Viral Infectious Diseases and Gene Expression in Insects
- Glycosylation and Glycoproteins Research
- Transgenic Plants and Applications
- Chemical Synthesis and Analysis
- CAR-T cell therapy research
- Peptidase Inhibition and Analysis
- Click Chemistry and Applications
- Enzyme Catalysis and Immobilization
- Enzyme Production and Characterization
- Advanced Biosensing Techniques and Applications
- RNA Interference and Gene Delivery
- Toxin Mechanisms and Immunotoxins
- HER2/EGFR in Cancer Research
- Bacterial Genetics and Biotechnology
- Immune Cell Function and Interaction
- Advanced biosensing and bioanalysis techniques
- Nanofabrication and Lithography Techniques
- Bacteriophages and microbial interactions
- Carbohydrate Chemistry and Synthesis
- Immunotherapy and Immune Responses
- Microbial Metabolic Engineering and Bioproduction
- Blood properties and coagulation
Technical University of Darmstadt
2016-2025
Hesse (Germany)
2024
Merck (Germany)
2017-2023
Bioengineering Center
2013
National Center for Genetic Engineering and Biotechnology
2013
Heinrich Heine University Düsseldorf
2011
University of Göttingen
1991-2007
Clemenshospital Münster
2007
Canadian Light Source (Canada)
2006
University of Saskatchewan
2006
Abstract Proximity-dependent biotinylation (PDB) combined with mass spectrometry analysis has established itself as a key technology to study protein-protein interactions in living cells. A widespread approach, BioID, uses an abortive variant of the E. coli BirA biotin protein ligase, quite bulky enzyme slow labeling kinetics. To improve PDB versatility and speed, various enzymes have been developed by different approaches. Here we present small-size engineered enzyme: ultraID. We show its...
Antibody-dependent cellular cytotoxicity plays a pivotal role in antibody-based tumor therapies and is based on the recruitment of natural killer cells to antibody-bound via binding Fcγ receptor III (CD16). Here we describe generation chimeric DNA aptamers that simultaneously bind CD16α c-Met, overexpressed many tumors. By application systematic evolution ligands by exponential enrichment (SELEX) method, specific were isolated bound with high specificity affinity (91 pm-195 nm) their...
In addition to antibodies with the classical composition of heavy and light chains, adaptive immune repertoire sharks also includes a heavy-chain only isotype, where antigen binding is mediated exclusively by small highly stable domain, referred as vNAR. recent years, due their high affinity specificity combined size, physicochemical stability low-cost production, vNAR fragments have evolved promising target-binding scaffolds that can be tailor-made for applications in medicine...
A good impression: modular approach using a ruthenium(II) catalyst during peptide synthesis gives rigid and well-defined triazole bridges as tailor-made substitutes for natural disulfide (see structures). The corresponding modification of the monocyclic sunflower trypsin inhibitor-1 yielded an equally potent peptidomimetic containing redox stable 1,5-disubstituted 1,2,3-triazole bridge.
Abstract Artificial cells are biomimetic microstructures that mimic functions of natural cells, can be applied as building blocks for molecular systems engineering, and host synthetic biology pathways. Here we report enzymatically synthesized polymer-based artificial with the ability to express proteins. were using biocatalytic atom transfer radical polymerization-induced self-assembly, in which myoglobin synthesizes amphiphilic block co-polymers self-assemble into structures such micelles,...
DegP and DegQ are homologous endoproteases found in the periplasmic compartment of Escherichia coli. The studies presented here suggest that have very similar substrate specificities cleave substrates which transiently or globally denatured. Model were cleaved at discrete Val/Xaa Ile/Xaa sites, suggesting aliphatic, beta-branched residues, typically buried hydrophobic core most proteins, important determinants cleavage specificity. Indeed, peptide bonds model generally inaccessible native...
Pseudomonas aeruginosa is both a ubiquitous environmental bacterium and an opportunistic human pathogen. A remarkable metabolic versatility allows it to occupy multitude of ecological niches, including wastewater treatment plants such hostile environments as the respiratory tract. P. able degrade metabolize biocidic SDS, detergent most commercial personal hygiene products. We identify SdsA1 secreted SDS hydrolase that use primary sulfates sole carbon or sulfur source. Homologues are found in...
Bispecific antibodies (bsAbs) and antibody-drug conjugates (ADCs) have already demonstrated benefits for the treatment of cancer in several clinical studies, showing improved drug selectivity efficacy. In particular, simultaneous targeting prominent antigens, such as EGF receptor (EGFR) c-MET, by bsAbs has raised increasing interest potentially circumventing cross-talk c-MET-mediated acquired resistance during anti-EGFR monotherapy. this study, we combined EGFR × c-MET with potency cytotoxic...
There is growing interest in the fast and robust engineering of protein pH-sensitivity that aims to reduce binding at acidic pH, compared neutral pH. Here, we describe a novel strategy for incorporation pH-sensitive antigen functions into antibody variable domains using combinatorial histidine scanning libraries yeast surface display. The allows simultaneous screening both, high affinity pH 7.4 pH-sensitivity, excludes conventional negative selection steps. As proof concept, applied this...
The Ecballium elaterium trypsin inhibitor II (EETI-II), a member of the squash family protease inhibitors, is composed 28 amino acid residues and potent trypsin. Its compact structure defined by triple-stranded antiparallel β-sheet, which held together three intramolecular disulfide bonds forming cystine knot. In order to explore potential EETI-II peptide serve as structural scaffold for presentation randomized oligopeptides, we constructed two derivatives, where six-residue loop was...
Getting a look in: A high-throughput screening method has been developed for the identification and isolation of enantioselective hydrolases displayed on cell surfaces (see scheme; E: Esterase, P: Peroxidase). Enantiomeric substrates labeled with two different fluorescent dyes allow real-time analysis enantioselectivity by determination ratio green red single-cell fluorescence. Detailed facts importance to specialist readers are published as "Supporting Information". Such documents...
Present in various species, the knottins (also referred to as inhibitor cystine knots) constitute a group of extremely stable miniproteins with plethora biological activities. Owing their small size and high stability, are considered excellent leads or scaffolds drug design. Two knottin families contain macrocyclic compounds, namely cyclotides squash inhibitors. The cyclotide family nearly exclusively contains head-to-tail cyclized members. On other hand, predominantly linear Head-to-tail...