A5026373390- Estrogen and related hormone effects
- Cytokine Signaling Pathways and Interactions
- Immune cells in cancer
- Immune Cell Function and Interaction
- Ferroptosis and cancer prognosis
- Cancer, Lipids, and Metabolism
- RNA modifications and cancer
- CAR-T cell therapy research
- Epigenetics and DNA Methylation
- T-cell and B-cell Immunology
- FOXO transcription factor regulation
- Cancer Genomics and Diagnostics
- Animal Virus Infections Studies
- Genetic diversity and population structure
- Genetics, Aging, and Longevity in Model Organisms
- Medical Imaging and Pathology Studies
- Cancer, Hypoxia, and Metabolism
- Viral Infections and Immunology Research
- Proteoglycans and glycosaminoglycans research
- Acute Lymphoblastic Leukemia research
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- Fibroblast Growth Factor Research
- Angiogenesis and VEGF in Cancer
- Prostate Cancer Treatment and Research
University of Minnesota
2015-2024
University of Minnesota System
2017-2024
University of Minnesota Medical Center
2013-2024
Twin Cities Orthopedics
2012-2024
Computational Physics (United States)
2020-2022
Pittsburgh Supercomputing Center
2020-2021
Toxicologie, Pharmacologie et Signalisation Cellulaire
2013
Minnesota Supercomputing Institute
2005-2009
North Carolina State University
2006
Roslin Institute
2006
Porcine circovirus 2 causes different clinical syndromes resulting in a significant economic loss the pork industry. Three pigs with unexplained cardiac and multi-organ inflammation that tested negative for PCV2 other known porcine pathogens were further analyzed. Histology was used to identify microscopic lesions multiple tissues. Metagenomics detect viral sequences tissue homogenates. In situ hybridization RNA expression tissue. all three cases we characterized genome of new called PCV3...
In 2015 and 2016, Senecavirus A (SVA) emerged as an infectious disease in Brazil, China the United States (US). a Colombian commercial swine farm, vesicles on snout coronary bands were reported tested negative for foot-and-mouth virus (FMDv), but positive SVA. The whole-genome phylogenetic analysis indicates strain clusters with strains from States, not recent SVA Brazil.
Abstract While macrophages are among the most abundant immune cell type found within primary and metastatic mammary tumors, how their complexity heterogeneity change with progression remains unknown. Here, were isolated from lungs of mice bearing orthotopic tumors for single-cell RNA sequencing (scRNA-seq). Seven distinct macrophage clusters identified, including populations exhibiting enhanced differential expression genes related to antigen presentation (H2-Aa, Cd74), cycle (Stmn1, Cdk1),...
Integration of external signals and B-lymphoid transcription factor activities organise B cell lineage commitment through alternating cycles proliferation differentiation, producing a diverse repertoire mature cells. We use single-cell transcriptomics/proteomics to identify differentially expressed gene networks across development correlate these with subtypes leukemia. Here we show unique transcriptional signatures that refine the pre-B expansion stages into pre-BCR-dependent...
Abstract Introduction Progesterone receptors (PR) are emerging as important breast cancer drivers. Phosphorylation events common to cells impact PR transcriptional activity, in part by direct phosphorylation. PR-B but not PR-A isoforms phosphorylated on Ser294 mitogen activated protein kinase (MAPK) and cyclin dependent 2 (CDK2). Phospho-Ser294 PRs resistant ligand-dependent Lys388 SUMOylation (that is, a repressive modification). Antagonism of small ubiquitin-like modifier (SUMO)ylation...
Estrogen and progesterone are potent breast mitogens. In addition to steroid hormones, multiple signaling pathways input estrogen receptor (ER) (PR) actions via posttranslational events. Protein kinases commonly activated in cancers phosphorylate hormone receptors (SRs) profoundly impact their activities. To better understand the role of modified PRs cancer, we measured total phospho-Ser294 209 human tumors represented on 2754 individual tissue spots within a microarray assayed regulation...
Tumor-associated macrophages contribute to tumor progression and therapeutic resistance in breast cancer. Within the microenvironment, tumor-derived factors activate pathways that modulate macrophage function. Using vitro vivo models, we find induce activation of Janus kinase (JAK)/signal transducer activator transcription 3 (STAT3) pathway macrophages. We also demonstrate loss STAT3 myeloid cells leads enhanced mammary tumorigenesis. Further studies show tumors JAK/STAT inhibitor...
CD8 effector T cells with a CD127
Abstract The epidemiology and genetic diversity of transmissible gastroenteritis virus (TGEV) in the United States (US) was investigated by testing clinical cases for TGEV real time RT-PCR between January 2008 November 2016. Prevalence ranged 3.8–6.8% peaked during cold months until March 2013, which prevalence decreased to < 0.1%. Nineteen complete genomes a single strain porcine respiratory coronavirus (PRCV) from US were generated compared historical strains investigate evolution these...
Retroviral and transposon-based mutagenesis screens in mice have been useful for identifying candidate cancer genes some tumor types. However, many of the organs that exhibit highest rates humans, including prostate, not previously amenable to these approaches. This study shows first time Sleeping Beauty transposon system can be used identify prostate mice. Somatic mobilization a mutagenic resulted focal epithelial proliferation hyperplasia prostate. Efficient methods were established...
Identification of unknown pathogens in pigs displaying enteric illness is difficult due to the large diversity bacterial and viral species found within faecal samples. Current methods often require or isolation, testing only a limited number known using quantitative PCR analysis. Herein, faeces from two 25-day-old piglets with diarrhoea Texas, USA, were analysed by metagenomic next-generation sequencing rapidly identify possible pathogens. Our analysis included bioinformatics pipeline rapid...
Progesterone receptors (PR) are transcription factors relevant to breast cancer biology. Herein, we describe an N-terminal common docking (CD) domain in PR-B, a motif first described mitogen-activated protein kinases. Binding studies revealed PR-B interacts with dual-specificity phosphatase 6 (DUSP6) via the CD domain. Mutation of (mCD) attenuated cell cycle progression and expression target genes (including STAT5A Wnt1); mCD failed undergo phosphorylation on Ser81, ck2-dependent site...
Cancer cells use stress response pathways to sustain their pathogenic behavior. In breast cancer, response-associated phenotypes are mediated by the tumor kinase, Brk (PTK6), via hypoxia-inducible factors HIF-1α and HIF-2α. Given that glucocorticoid receptor (GR) is highly expressed in triple-negative cancer (TNBC), we investigated cross-talk between hormone-driven GR signaling HIF-regulated physiologic stress. Primary TNBC explants or cell lines treated with ligand dexamethasone exhibited...
Cancer has been conceptualized as a chronic wound with predominance of tumor promoting inflammation. Given the accumulating evidence that microenvironment supports growth, we investigated hyaluronan (HA)-CD44 interactions within breast cancer cells, to determine whether this axis directly impacts formation an inflammatory microenvironment. Our results demonstrate cells synthesize and fragment HA express CD44 on cell surface. Using RNA sequencing approaches, found loss in altered expression...
The progesterone receptor (PR) and its coactivators are direct targets of activated cyclin-dependent kinases (CDKs) in response to peptide growth factors, progesterone, deregulation cell cycle inhibitors. Herein, using the T47D breast cancer model, we probed mechanisms cycle–dependent PR action. In absence exogenous progestin, is specifically phosphorylated during G2/M phase. Accordingly, numerous target genes regulated, including HSPB8, a heat-shock protein whose high expression associated...
Abstract Androgen receptor (AR) inhibition is standard of care for advanced prostate cancer (PC). However, efficacy limited by progression to castration-resistant PC (CRPC), usually due AR re-activation via mechanisms that include amplification and structural rearrangement. These two classes alterations often co-occur in CRPC tumors, but it unclear whether this reflects intercellular or intracellular heterogeneity AR. Resolving important developing new therapies predictive biomarkers. Here,...
Breast cancer invasion and metastasis result from a complex interplay between tumor cells the microenvironment (TME). Key oncogenic changes in TME include aberrant synthesis, processing, signaling of hyaluronan (HA). Hyaluronan-mediated motility receptor (RHAMM, CD168; HMMR) is an HA enabling to sense respond this during breast progression. Previous studies have associated RHAMM expression with progression; however, cause effect mechanisms are incompletely established. Focused gene analysis...
Genetic markers (microsatellites and SNPs) were used to create compare maps of the turkey chicken genomes. A physical map genome was built by comparing sequences with whole-genome sequence BLAST analysis. genetic linkage <i>(Meleagris gallopavo)</i> developed segregation analysis within University Minnesota/Nicholas Turkey Breeding Farms (UMN/NTBF) resource population. This includes 314 loci arranged into 29 groups. An additional 40 are tentatively placed groups based on...