A5101789588- Plant tissue culture and regeneration
- RNA Research and Splicing
- Advanced biosensing and bioanalysis techniques
- Epigenetics and DNA Methylation
- Lipid Membrane Structure and Behavior
- Genomics and Chromatin Dynamics
- Innovative Microfluidic and Catalytic Techniques Innovation
- Cancer Immunotherapy and Biomarkers
- Mitochondrial Function and Pathology
- Oral and Maxillofacial Pathology
- Herbal Medicine Research Studies
- Electrochemical Analysis and Applications
- Metabolism and Genetic Disorders
- Analytical Chemistry and Sensors
- Single-cell and spatial transcriptomics
- Cancer-related Molecular Pathways
- Ion Channels and Receptors
- Wnt/β-catenin signaling in development and cancer
- Sulfur Compounds in Biology
- Signaling Pathways in Disease
- Ferroptosis and cancer prognosis
- Ultrasound Imaging and Elastography
- Cellular Mechanics and Interactions
- Molecular Sensors and Ion Detection
- RNA Interference and Gene Delivery
Central South University
2014-2022
Fujian Medical University
2020-2022
Xiangya Hospital Central South University
2014-2022
Jiangxi Provincial People's Hospital
2022
Nanchang University
2022
Xuzhou No.1 People's Hospital
2022
Xuzhou Medical College
2022
Shandong Normal University
2015-2019
Jilin University
2015
Purdue University West Lafayette
2015
Here, a non-destructive amplification strategy is developed to image miRNAs in living cells, utilizing the enzyme-free hybridization chain reaction (HCR) with graphene oxide (GO) as carrier. This provides new tool for highly sensitive and simultaneous imaging of multiple low-level biomarkers, improving accuracy early disease diagnosis.
Idiopathic pulmonary fibrosis is a progressive fibrotic disease characterized by excessive deposition of (myo)fibroblast produced collagen fibrils in alveolar areas the lung. Lysyl oxidases (LOXs) have been proposed to be central enzymes that catalyze cross-linking fibers. Here, we report that, while its expression increased lungs, genetic ablation LOXL2 only leads modest reduction pathological but not On other hand, loss another LOX family member, LOXL4, markedly disrupts and Furthermore,...
Although laboratory studies have implicated the high mobility group box 1 (HMGB1) in melanoma, its clinical relevance remains unclear. We analyzed nearly 100 cases of human melanoma and found that HMGB1 was highly overexpressed samples relative to normal skin nevi tissues. Significantly, higher levels correlated with more advanced disease stages poorer survival patients. Unlike well-documented pro-inflammatory role extracellular HMGB1, we intracellular activity is necessary for cell...
A sensitive, label-free immunosensor based on iridium oxide (IrOx, 0 ≤ x 2) nanofibers, which were synthesized through a simple one-spinneret electrospinning method, was first developed for immunoassay of the cancer biomarker α-fetoprotein (AFP). The specific wire-in-tube nanostructure could be obtained and composition IrOx nanofibers also controlled changing annealing temperature. unique structure properties at 500 °C not only led to increased electrode surface area accelerated electron...
Considering the important functions of cellular Na+ and K+ together with their cooperative efforts on various biological processes, it is significant to simultaneously detect at a single-cell level. Here, we present novel method discriminate quantify in single cells using new near-infrared fluorescent probe associated microchip electrophoresis. The selectively responds both K+. electrophoresis allows accurate manipulation effective distinction Based method, concentration normal cancer was...
Article25 May 2022Open Access Source DataTransparent process Stress-induced vesicular assemblies of dual leucine zipper kinase are signaling hubs involved in activation and neurodegeneration Elena Tortosa orcid.org/0000-0003-3910-2314 Department Neuroscience, Genentech, Inc., South San Francisco, CA, USA Contribution: Conceptualization, Data curation, Software, Formal analysis, Supervision, Validation, Investigation, Visualization, Methodology, Writing - original draft, Project...
As important reactive oxygen species (ROS) and nitrogen (RNS), cellular superoxide anion (O2(•-)) nitric oxide (NO) play significant roles in numerous physiological pathological processes. Cellular O2(•-) NO also have a close relationship always interact with each other. Thus, the simultaneous detection of intracellular NO, especially at single-cell level, is important. In this paper, we present novel method to simultaneously detect quantify single cells using microchip electrophoresis based...
Various intracellular metal ions have closely related functional roles in the nervous system. An excess or deficiency of essential can contribute to neurodegenerative diseases. Thus, detection various neurons is important for diagnosing and monitoring these In particular, single-cell analysis multiple allows us not only understand cellular heterogeneity differentiation but also determine actual relationships among each individual cell. Aiming at low efficient manipulation interference...
We established an efficient method for single-cell multiple miRNA analysis by droplet microfluidics with high sensitivity and throughput.
Seven in absentia homolog 1 (Siah1) has been shown plays important roles the pathogenesis and development of multiple cancers. However, functions mechanisms Siah1 non-small cell lung cancer (NSCLC) remain unclear. In our study, we found that knock down could inhibit proliferation NSCLC cells, while over-expression had opposite effects. Molecularly, bioinformatics analysis determined notch receptor (Notch1) might be potential target Siah1. Subsequently, identified acted as an E3 ligase to...
We investigated the effects of various concentrations diethyl aminoethyl hexanoate (DA-6) on regeneration and growth adventitious buds in vitro purple coneflower cultures. Among 3 types explants tested, leaf required higher DA-6 than petiole root order to obtain high rates, while lowest concentration DA-6. Additionally, with ploidy levels were more sensitive addition DA-6, lower achieve its maximal rate. Interestingly, application a that was conducive inhibitory levels. Moreover, during...
Abstract Bird song is typically depicted as a male singing long‐distance signal to potentially unknown receivers (1) deter males and (2) attract females. Nevertheless, many songbirds sing from close distances known receiver; of these species may be under more intense selective pressure modify their songs depending on the sex receiver in order convey different motivational states (aggression versus courtship) sexes. In laboratory setting, we examined how affected within‐song variation...
A strategy of repeatedly inducing axillary bud formation from explants was tested for micro propagation purple coneflower (Echinacea purpurea L.) different gene dosages. It found that lower dosage required concentrations cytokinin (BA) and auxin (NAA) to achieve higher rate buds, with the most suitable BA NAA tested, haploid had much than tetraploid, while diploid closer tetraploid. By established culture protocol, about 1,500,000 haploid, 1,200,000 600,000 tetraploid plantlets can be...
Introduction RALA is a member of the small GTPase Ras superfamily and has been shown to play role in promoting cell proliferation migration most tumors, increase resistance anticancer drugs such as imatinib cisplatin. Although many literatures have studied cancer-promoting mechanism RALA, there lack relevant pan-cancer analysis. Methods This study systematically analyzed differential expression mutation pan-cancer, including different tissues cancer lines, prognosis immune infiltration...