A5040838377- Viral Infections and Immunology Research
- Immune cells in cancer
- Transplantation: Methods and Outcomes
- Cervical Cancer and HPV Research
- Congenital heart defects research
- Cancer Immunotherapy and Biomarkers
- Atherosclerosis and Cardiovascular Diseases
- Cancer Genomics and Diagnostics
- Aortic aneurysm repair treatments
- Animal Virus Infections Studies
- Signaling Pathways in Disease
- COVID-19 Clinical Research Studies
- SARS-CoV-2 and COVID-19 Research
- Interstitial Lung Diseases and Idiopathic Pulmonary Fibrosis
- Cardiac Fibrosis and Remodeling
- CAR-T cell therapy research
- HER2/EGFR in Cancer Research
- Breast Cancer Treatment Studies
- Liver Disease Diagnosis and Treatment
- Tumors and Oncological Cases
- T-cell and B-cell Immunology
- Aortic Disease and Treatment Approaches
- Immune Cell Function and Interaction
- Cell Adhesion Molecules Research
- Connective tissue disorders research
Washington University in St. Louis
2013-2024
Bipar
2024
Mallinckrodt (United States)
2022-2023
Duke University
2023
Brigham and Women's Hospital
2023
Alvin J. Siteman Cancer Center
2023
University of Minnesota Rochester
2022
Emory University
2022
Roche (Switzerland)
2022
Yale University
2022
There is ongoing debate as to whether cardiac complications of coronavirus disease-2019 (COVID-19) result from myocardial viral infection or are secondary systemic inflammation and/or thrombosis. We provide evidence that cardiomyocytes infected in patients with COVID-19 myocarditis and susceptible severe acute respiratory syndrome 2. establish an engineered heart tissue model pathology, define mechanisms pathogenesis, demonstrate cardiomyocyte 2 results contractile deficits, cytokine...
Immune checkpoint inhibitors (ICIs), antibodies targeting PD-1 (programmed cell death protein 1)/PD-L1 death-ligand 1) or CTLA4 (cytotoxic T-lymphocyte-associated 4), have revolutionized cancer management but are associated with devastating immune-related adverse events including myocarditis. The main risk factor for ICI myocarditis is the use of combination and inhibition. often fulminant pathologically characterized by myocardial infiltration T lymphocytes macrophages. Although much has...
Current models of B lymphocyte biology posit that cells continuously recirculate between lymphoid organs, without accumulating in peripheral healthy tissues. Nevertheless, lymphocytes are one the most prevalent leukocyte populations naive murine heart. To investigate this apparent inconsistency literature, we conducted a systematic analysis myocardial cell ontogeny, trafficking dynamics, histology, and gene expression patterns. We found represent subpopulation circulating make close contact...
Cardiac involvement is an important determinant of mortality among sarcoidosis patients. Although granulomatous inflammation a hallmark finding in cardiac sarcoidosis, the precise immune cell populations that comprise granuloma remain unresolved. Furthermore, it unclear how cellular and transcriptomic landscape differs from other inflammatory heart diseases.
Cellular rejection after heart transplantation imparts significant morbidity and mortality. Current immunosuppressive strategies are imperfect, target recipient T cells, have adverse effects. The innate immune response plays an essential role in the recruitment activation of cells. Targeting donor would represent earliest interventional opportunity within cascade. There is limited knowledge about cell types functions setting cardiac transplantation, no current therapeutics exist for...
Non–small cell lung cancer (NSCLC) is a leading cause of cancer-related deaths. Immune checkpoint blockade has improved survival for many patients with NSCLC, but most fail to obtain long-term benefit. Understanding the factors reduced immune surveillance in NSCLC critical improving patient outcomes. Here, we show that human harbors large amounts fibrosis correlates T infiltration. In murine models, induction led increased progression, impaired surveillance, and failure efficacy. Associated...
Tissue-resident macrophages are complementary to proinflammatory promote the progression of atherosclerosis. The noninvasive detection their presence and dynamic variation will be important understanding role in pathogenesis goal this study was develop a targeted PET radiotracer for imaging CD163-positive (CD163+) multiple mouse atherosclerosis models assess potential CD163 as biomarker humans. <b>Methods:</b> CD163-binding peptide identified using phage display conjugated with NODAGA...
Brain metastases can occur in nearly half of patients with early and locally advanced (stage I-III) non-small cell lung cancer (NSCLC). There are no reliable histopathologic or molecular means to identify those who likely develop brain metastases. We sought determine if deep learning (DL) could be applied routine H&E-stained primary tumor tissue sections from stage I-III NSCLC predict the development metastasis. Diagnostic slides 158 followed for at least 5 years (Met
Development of the cerebral vessels, pharyngeal arch arteries (PAAs). and cardiac outflow tract (OFT) requires multipotent neural crest cells (NCCs) that migrate from tube to target tissue destinations. Little is known about how mammalian NCC development orchestrated by gene programming at chromatin level, however. Here we show Brahma-related 1 (Brg1), an ATPase subunit Brg1/Brahma-associated factor (BAF) chromatin-remodeling complex, required in NCCs direct cardiovascular development. Mouse...
Elastin is an important ECM (extracellular matrix) protein in large and small arteries. Vascular smooth muscle cells (SMCs) produce the layered elastic laminae found arteries but synthesize little elastin muscular However, have a well-defined internal lamina (IEL) that separates endothelial (ECs) from SMCs. The extent to which ECs contribute IEL unknown.To use targeted (Eln) deletion mice explore relative contributions of SMCs formation different arteries.We used SMC- EC-specific Cre...
The monocyte chemoattractant protein-1/CCR2 (chemokine receptor 2) axis plays an important role in abdominal aortic aneurysm (AAA) pathogenesis, with effects on disease progression and anatomic stability. We assessed the expression of CCR2 a rodent model human tissues, using targeted positron emission tomography radiotracer (
COVID-19 is associated with serious cardiovascular complications, incompletely understood mechanism(s). Pericytes have key functions in supporting endothelial cells and maintaining vascular integrity. We demonstrate that human cardiac pericytes are permissive to SARS-CoV-2 infection organotypic slice primary cell cultures. Viral entry into mediated by endosomal proteases, leads up-regulation of inflammatory markers, vasoactive mediators, nuclear factor kappa-B-dependent death. Furthermore,...
Polymerase chain reaction (PCR) was used to amplify and identify the presence of DNA human papillomavirus (HPV) types 6, 11, 16, 18 in peripheral blood mononuclear cells (PBMCs) women with without urogenital HPV infections. various found PBMCs 13 25 (52.0%) patients infections none 19 control subjects who are free The may impair immunologic functions lymphocytes play a role epidemiology pathogenesis HPVinduced diseases.
Tumor‐associated macrophages play an important role in tumor progression, but whether they exert a tumor‐progressive effect remains controversial. Here, we demonstrated that activated macrophage‐conditioned medium (AMCM) obtained from RAW (RAW/AMCM) induced epithelial‐mesenchymal transition (EMT) and stimulated the migratory invasive activities of HepG2 cells, whereas control conditioned media had no effect. Epithelial‐cadherin (E‐cadherin) β‐catenin staining patterns were altered at...
Hypoxia-induced pulmonary hypertension (PH) is one of the most common and deadliest forms PH. Fibroblast growth factor receptors 1 2 (FGFR1/2) are elevated in patients with PH mice exposed to chronic hypoxia. Endothelial FGFR1/2 signaling important for adaptive response several injury types we hypothesized that endothelial would protect against hypoxia-induced Mice lacking FGFR1/2, activated FGFR signaling, human artery cells (HPAECs) were challenged We assessed effect activation inhibition...
Background Viral myocarditis is characterized by leukocyte infiltration of the heart and cardiomyocyte death. We recently identified C-C chemokine ligand (CCL) 17 as a proinflammatory effector receptor 2-positive macrophages dendritic cells that are recruited to contribute adverse left ventricular remodeling following myocardial infarction pressure overload. Methods Results Mouse encephalomyocarditis virus was used investigate function CCL17 in viral model. Ccl17Gfp reporter knockout mice...