A5010885969- SARS-CoV-2 and COVID-19 Research
- Insect Resistance and Genetics
- Viral Infectious Diseases and Gene Expression in Insects
- Ion Channels and Receptors
- Invertebrate Immune Response Mechanisms
- Sodium Intake and Health
- Insect symbiosis and bacterial influences
- Entomopathogenic Microorganisms in Pest Control
- Viral gastroenteritis research and epidemiology
- CAR-T cell therapy research
- COVID-19 Clinical Research Studies
- Mosquito-borne diseases and control
- Diverse Education Studies and Reforms
- Influenza Virus Research Studies
- Monoclonal and Polyclonal Antibodies Research
- Viral Infections and Immunology Research
- Dermatologic Treatments and Research
- Cardiovascular, Neuropeptides, and Oxidative Stress Research
- Virus-based gene therapy research
- Viral Infections and Outbreaks Research
- Calcium signaling and nucleotide metabolism
- Viral Infections and Vectors
- RNA Interference and Gene Delivery
- Coenzyme Q10 studies and effects
- Global Educational Reforms and Inequalities
Army Medical University
2019-2024
Daping Hospital
2019-2024
Pioneer (United States)
2024
Peking University
2024
University of Tsukuba
2024
University of Chinese Academy of Sciences
2017-2022
Chinese Academy of Sciences
2017-2022
Wuhan Institute of Virology
2017-2022
Chongqing Medical University
2020
Second Affiliated Hospital of Chongqing Medical University
2020
Melanization, an important insect defense mechanism, is mediated by clip-domain serine protease (cSP) cascades and regulated serpins. Here we show that proteolytic activation of prophenoloxidase (PPO) PO-catalyzed melanization kill the baculovirus in vitro. Our quantitative proteomics biochemical experiments revealed infection cotton bollworm, Helicoverpa armigera, reduced levels most cascade members host hemolymph PO activity. By contrast, serpin-9 serpin-5 were sequentially upregulated...
The rapid emergence and spread of escaping mutations severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has significantly challenged our efforts in fighting against the COVID-19 pandemic. A broadly neutralizing reagent these concerning variants is thus highly desirable for prophylactic therapeutic treatments SARS-CoV-2 infection. We herein report a covalent engineering strategy on protein minibinders potent neutralization such as B.1.617.2 (Delta), B.1.617.1 (Kappa), B.1.1.529...
Melanization is a prominent insect humoral response for encapsulation of and killing invading pathogens. It mediated by protease cascade composed modular serine (SP), clip domain SPs (cSPs), which converts prophenoloxidase (PPO) into active phenoloxidase (PO). To date, melanization pathway in cotton bollworm Helicoverpa armigera, an important agricultural pest, remains largely unclear. biochemically reconstitute the vitro, putative proteases along with modified containing factor Xa cleavage...
The global emergence of SARS-CoV-2 variants has led to increasing breakthrough infections in vaccinated populations, calling for an urgent need develop more effective and broad-spectrum vaccines combat COVID-19. Here we report the preclinical development RQ3013, mRNA vaccine candidate intended bring broad protection against concern (VOCs). which contains pseudouridine-modified mRNAs formulated lipid nanoparticles, encodes spike (S) protein harboring a combination mutations responsible immune...
A multitude of functional mutations continue to emerge on the N-terminal domain (NTD) spike protein in SARS-CoV-2 Omicron subvariants. Understanding immunogenicity NTD and properties antibodies elicited by it is crucial for comprehending impact viral fitness guiding vaccine design. In this study, we find that most NTD-targeting isolated from individuals with BA.5/BF.7 breakthrough infection (BTI) are ancestral (wildtype or WT)-reactive non-neutralizing. Surprisingly, identified five...
Diet High in salt content have been associated with cardiovascular disease and chronic inflammation. We recently demonstrated that transient receptor potential canonical 3 (TRPC3) channels regulate myofibroblast transdifferentiation hypertrophic scars. Here, we examined how high activation of TRPC3 participates scarring during wound healing. In vitro, confirmed increased the protein expression marker alpha smooth muscle actin (α-SMA) wild-type mice (WT) primary cultured dermal fibroblasts...
The renal proximal tubule (RPT) plays a pivotal role in regulating sodium reabsorption and thus blood pressure (BP). Transient receptor potential ankyrin 1 (TRPA1) has been reported to protect against injury by modulating mitochondrial function. We hypothesize that the activation of TRPA1 its agonist cinnamaldehyde may mitigate high-salt intake-induced hypertension inhibiting urinary through restoration tubular epithelial
Oxyplax ochracea (Moore) is a pest that causes severe damage to wide range of crops, forests and fruit trees. The complete genome sequence nucleopolyhedrovirus (OxocNPV) was determined using Roche 454 pyrosequencing system. OxocNPV has double-stranded DNA (dsDNA) 113,971 bp with G+C content 31.1%. One hundred twenty-four putative open reading frames (ORFs) encoding proteins >50 amino acids in length minimal overlapping were predicted, which covered 92% the whole genome. Six baculoviral...
Abstract The global emergence of SARS-CoV-2 variants has led to increasing breakthrough infections in vaccinated populations, calling for an urgent need develop more effective and broad-spectrum vaccines combat COVID-19. Here we report the preclinical development RQ3013, mRNA vaccine candidate intended bring broad protection against concern (VOCs). which contains pseudouridine-modified mRNAs formulated lipid nanoparticles, encodes spike(S) protein harboring a combination mutations...
Abstract This article introduces Kominkan and its practice on Japanese social education how it facilitates adult lifelong learning in local communities. Historically, was understood as all that promotes development societal progress. Kominkan, institutions for citizens, contribute to community resolve issues problems through self‐determined learning. Nowadays, is declining mainly because of administration reasons. However, the need restoring recognized reconfirmed practice. Through...
Artaxa digramma is a lepidopteran pest distributed throughout southern China, Myanmar, Indonesia, and India. nucleopolyhedrovirus (ArdiNPV) specific viral pathogen of A. deemed as promising biocontrol agent against the pest. In this study, complete genome sequence ArdiNPV was determined by deep sequencing. The contains double-stranded DNA (dsDNA) 161,734 bp in length 39.1% G+C content. Further, 149 hypothetical open reading frames (ORFs) were predicted to encode proteins >50 amino acids...
Objective . Myofibroblast transformation has been shown to be associated with the reactive oxygen species- (ROS-) producing enzyme NADPH oxidase (Nox4). Inhibition of transient receptor potential channel canonical type 3 (TRPC3) attenuates mitochondrial calcium handling and ROS production in vasculature hypertensive rats. However, it remains elusive whether TRPC3 regulates participates myofibroblast transdifferentiation during wound healing. Methods Results In this study, we demonstrated...
Abstract P26, a homolog of the viral-encoded nuclease poxin that neutralizes cGAS-STING innate immunity, is widely distributed in various invertebrate viruses, lepidopteran insects, and parasitoid wasps. P26/poxin from certain insect viruses also retains protease activity, though its biological role remains unknown. Given many P26s contain signal peptide, it surmised P26 may possess extracellular functions. Here, we report secretory baculoviral suppresses melanization, prominent immunity...
Neutralizing monoclonal antibodies (mAb) are a major therapeutic strategy for the treatment of severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) infection. The continuous emergence new SARS-CoV-2 variants worldwide has increased urgency development mAbs. In this study, we immunized mice with receptor-binding domain (RBD) prototypic strain (WIV04) and screened 35 RBD-specific mAbs using hybridoma technology. Results plaque reduction neutralization test showed that 25 neutralized...
Abstract Japan, like many countries, is facing problems with an aging society, and lifelong learning becoming more important. To provide older adults the opportunity to enroll in programs, it essential offer suitable programs. However, designing programs for not easy because they may have various physical mental conditions. approach problem, authors proposed a mechanism personalizing adults. The basic ideas of are: (1) omitting unessential steps (2) suggesting collaboration others. In this...
Abstract A multitude of functional mutations continue to emerge on the N-terminal domain (NTD) spike protein in SARS-CoV-2 Omicron subvariants. Understanding immunogenicity NTD and properties antibodies elicited by it is crucial for comprehending impact viral fitness guiding vaccine design. In this study, we find that most NTD-targeting isolated from individuals with BA.5/BF.7 breakthrough infection (BTI) are ancestral (wildtype or WT)-reactive non-neutralizing. Surprisingly, identified five...