A5043366713- CRISPR and Genetic Engineering
- RNA and protein synthesis mechanisms
- Viral Infections and Immunology Research
- Probiotics and Fermented Foods
- Cytomegalovirus and herpesvirus research
- Advanced biosensing and bioanalysis techniques
- Gut microbiota and health
- SARS-CoV-2 and COVID-19 Research
- Bacterial Genetics and Biotechnology
- RNA Interference and Gene Delivery
- Computability, Logic, AI Algorithms
- Protein Hydrolysis and Bioactive Peptides
- Insect symbiosis and bacterial influences
- Viral gastroenteritis research and epidemiology
- RNA regulation and disease
- Microbial Metabolites in Food Biotechnology
- Genomics and Rare Diseases
- Water Quality Monitoring Technologies
- melanin and skin pigmentation
- Cancer Research and Treatments
- Advanced Electron Microscopy Techniques and Applications
- Tea Polyphenols and Effects
- Virus-based gene therapy research
- Electron and X-Ray Spectroscopy Techniques
- Wastewater Treatment and Nitrogen Removal
University of Copenhagen
2017-2025
Fudan University
2018-2024
Zhongshan Hospital
2022-2024
Sun Yat-sen University
2023
The First Affiliated Hospital, Sun Yat-sen University
2023
Shandong University
2021
Huashan Hospital
2018
State Key Laboratory of Food Science and Technology
2015-2016
Jiangnan University
2016
Huazhong Agricultural University
2014-2015
The global emergence of SARS-CoV-2 variants has led to increasing breakthrough infections in vaccinated populations, calling for an urgent need develop more effective and broad-spectrum vaccines combat COVID-19. Here we report the preclinical development RQ3013, mRNA vaccine candidate intended bring broad protection against concern (VOCs). which contains pseudouridine-modified mRNAs formulated lipid nanoparticles, encodes spike (S) protein harboring a combination mutations responsible immune...
Abstract Aspirin, also named acetylsalicylate, can directly acetylate the side‐chain of lysine in protein, which leads to possibility unexplained drug effects. Here, study used isotopic‐labeling aspirin‐d 3 with mass spectrometry analysis discover that aspirin acetylates 10 HDACs proteins, including SIRT1, most studied NAD + ‐dependent deacetylase. SIRT1 is acetylated by vitro. It identified 408 abolishes deacetylation activity impairing substrates binding affinity. Interestingly, be CBP...
Prokaryotic CRISPR-Cas immune systems are classified into six types based on their effector complexes which cleave dsDNA specifically (types I, II and V), ssRNA exclusively (type VI) or both via a ruler mechanism ssDNA unspecifically III). To date, no specific cleavage of target has been reported for CRISPR-Cas. Here, we demonstrate dual activities subtype I-D system carries type III Cas10-like large subunit, Cas10d. In addition to activity dependent the HD domain Cas10d, helicase Cas3'...
Antiviral defense by type III CRISPR-Cas systems relies on two distinct activities of their effectors: the RNA-activated DNA cleavage and synthesis cyclic oligoadenylate. Both are featured as indiscriminate nucleic acid subjected to spatiotemporal regulation. To yield further insights into involved mechanisms, we reconstituted LdCsm, a lactobacilli III-A system in Escherichia coli. Upon activation target RNA, this immune mediates robust degradation but lacks oligoadenylates. Mutagenesis Csm3...
Abstract A hallmark of type I CRISPR–Cas systems is the presence Cas3, which contains both nuclease and helicase activities required for DNA cleavage during interference. In subtype I-D systems, however, histidine-aspartate (HD) domain encoded as part a Cas10-like large effector complex subunit activity in separate Cas3’ subunit, but functional mechanistic consequences this organisation are not currently understood. Here we show that Sulfolobus islandicus Cas10d exhibits an unusual...
CRISPR-Cas (clustered regularly interspaced short palindromic repeats-CRISPR-associated) systems provide adaptive immunity against invasive nucleic acids guided by CRISPR RNAs (crRNAs) in archaea and bacteria. Type III effector complexes show RNA cleavage RNA-activated DNA activity, representing the only known system of dual acid interference. Here, we investigated function Cmr1 genetic assays interference activity mutants biochemical characterization their mutated Cmr complexes. Three cmr1α...
Heterologous vaccine schedules have been recommended to provide superior immunity and protection against emergent SARS-CoV-2 variants of concern. We aimed evaluate the safety, immunogenicity, efficacy an mRNA COVID-19 RQ3013 compared with adenoviral vectored Ad5-nCoV protein subunit ZF2001 as fourth dose in adults primed three doses inactivated vaccines China.
Many type III CRISPR-Cas systems rely on the cyclic oligoadenylate (cOA) signaling pathway to exert immunization. However, LdCsm, a III-A lactobacilli immune system mediates efficient plasmid clearance in spite of lacking cOA signaling. Thus, provides good model for detailed characterization RNA-activated DNase vitro and vivo. We found ATP functions as ligand enhance LdCsm ssDNase, enhancement is essential vivo clearance. In assays demonstrated cleaved transcriptional bubbles at any...
The aim of this study was to investigate the influence proteolytic pork hydrolysate (PPH) on yoghurt production by Lactobacillus delbrueckii subsp. bulgaricus and Streptococcus thermophilus. Fresh lean cut into pieces mixed with deionized water dealt protease, then resulting PPH added milk effects production. fermentation time, viable cell counts, flavor, free amino acids compounds, sensory evaluation were evaluated. These results showed that significantly stimulated growth acidification...
Reporter gene systems are useful for studying bacterial molecular biology, including the regulation of expression and histochemical analysis protein products. Here, two genes, β-1,4-mannanase (manB) from Bacillus pumilus β-glucuronidase (gusA) Escherichia coli K12, were cloned into vector pELX1. The patterns these reporter genes in Lactobacillus casei investigated by measuring their enzymatic activities estimating recombinant yields using western blot analysis. Whereas mannanase activity was...
The membrane bioreactor (MBR) system has become more and attractive in the field of wastewater treatment. It is particularly situations where long solids retention times are required, such as nitrifying bacteria, physical critical to achieving efficiency for biological degradation pollutant. Although it a new technology, MBR process been applied industrial treatment only past decade. opto-electronic industry, developed very fast over decade world, high technology manufacturing. containing...
Clustered regularly interspaced short palindromic repeats (CRISPR) and CRISPR-associated (Cas) systems provide the adaptive antiviral immunity against invasive genetic elements in archaea bacteria. These immune are divided into at least six different types, among which Type III CRISPR–Cas show several distinct activities as demonstrated from investigation of bacterial III-A archaeal III-B past decade. First, although initial experiments suggested that provided DNA interference activity,...
Abstract The global emergence of SARS-CoV-2 variants has led to increasing breakthrough infections in vaccinated populations, calling for an urgent need develop more effective and broad-spectrum vaccines combat COVID-19. Here we report the preclinical development RQ3013, mRNA vaccine candidate intended bring broad protection against concern (VOCs). which contains pseudouridine-modified mRNAs formulated lipid nanoparticles, encodes spike(S) protein harboring a combination mutations...
Type III CRISPR-Cas systems show the target (tg)RNA-activated indiscriminate DNA cleavage and synthesis of oligoadenylates (cOA) a secondary signal that activates downstream nuclease effectors to exert RNA/DNA cleavage, both activities are regulated in spatiotemporal fashion. In III-B Cmr systems, cognate tgRNAs activate two Cmr2-based activities, which then inactivated via tgRNA by Cmr4, but how Cmr4 regulates immunization remains be experimentally characterized. Here, we conducted...
With the continuing development of sequencing technology, genomics has been applied in a variety biological research areas. In particular, application to marine species, which boast high diversity, promises great scientific and industrial potential. Significant progress made especially over past few years. Consequently, BGI, leveraging its prominent contributions research, established BGI-Qingdao, an institute specifically aimed at exploring genomics. order accelerate related applications,...
We previously reported that the β-1,4-Mannanase (manB) gene from Bacillus pumilus functions as a good reporter in Lactobacillus casei. Two vectors were constructed. One carries signal peptide of secretion protein Usp45 (SPUsp45) Lactococcus lactis (pELSH), and other full-length S-layer protein, SlpA, L. acidophilus (pELWH). In this work, another vector, pELSPH, was constructed to include SlpA (SPSlpA), capacity all three drive expression manB casei evaluated. The results showed SPUsp45 is...
Abstract Antiviral defense by type III CRISPR-Cas systems relies on two distinct activities of their effectors: the RNA-activated DNA cleavage and synthesis cyclic oligoadenylate. Both are featured as indiscriminate nucleic acid subjected to spatiotemporal regulation. To yield further insights into involved mechanisms, we reconstituted LdCsm, a lactobacilli III-A system in Escherichia coli . Upon activation target RNA, this immune mediates robust degradation but lacks oligoadenylates....