A5045364623- Cardiac electrophysiology and arrhythmias
- Circular RNAs in diseases
- Virus-based gene therapy research
- MicroRNA in disease regulation
- RNA modifications and cancer
- Ion channel regulation and function
- RNA Research and Splicing
- CRISPR and Genetic Engineering
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Cancer-related molecular mechanisms research
- Neuroscience and Neuropharmacology Research
- DNA Repair Mechanisms
- RNA Interference and Gene Delivery
- Biotin and Related Studies
- Receptor Mechanisms and Signaling
- Insect Resistance and Genetics
- Cellular transport and secretion
- Plant Virus Research Studies
- Pluripotent Stem Cells Research
- Cancer-related Molecular Pathways
- Click Chemistry and Applications
- Sirtuins and Resveratrol in Medicine
Medical Process (France)
2025
Zhejiang University of Science and Technology
2025
East China University of Science and Technology
2023-2024
Center for Life Sciences
2021-2023
Peking University
2021-2023
Columbia University Irving Medical Center
2020-2022
Jiangsu Normal University
2020-2021
Henan Agricultural University
2020
Zhejiang University
2017-2018
Nankai University
2018
The targeted degradation of membrane proteins would afford an attractive and general strategy for treating various diseases that remain difficult with the current proteolysis-targeting chimera (PROTAC) methodology. We herein report a covalent nanobody-based PROTAC strategy, termed GlueTAC, protein high specificity efficiency. first established mass-spectrometry-based screening platform rapid development nanobody (GlueBody) allowed proximity-enabled cross-linking surface antigens on cancer...
Spatiotemporally resolved dissection of subcellular proteome is crucial to our understanding cellular functions in health and disease. We herein report a bioorthogonal photocatalytic decaging-enabled proximity labeling strategy (CAT-Prox) for spatiotemporally mitochondrial profiling living cells. Our systematic survey the photocatalysts has led identification Ir(ppy)2bpy as mitochondria-targeting catalyst that allowed photocontrolled, rapid rescue azidobenzyl-caged quinone methide highly...
Midbrain dopaminergic (mDA) neurons exhibit extensive dendritic and axonal arborizations, but local protein synthesis is not characterized in these neurons. Here, we investigate messenger RNA (mRNA) localization translation mDA neuronal axons dendrites, both of which release dopamine (DA). Using highly sensitive ribosome-bound sequencing imaging approaches, find no evidence for mRNA axons. In contrast, dendrites the substantia nigra pars reticulata (SNr) contain ribosomes mRNAs encoding...
Abstract Human pluripotent stem cells (hPSCs) have potential applications in biological studies and regenerative medicine. However, precise genome editing hPSCs remains time-consuming labor-intensive. Here we demonstrate that the recently identified CRISPR-Cpf1 can be used to efficiently generate knockout knockin hPSC lines. The unique properties of CRISPR-Cpf1, including shorter crRNA length low off-target activity, are very attractive for many applications. In particular, develop an...
The rapid emergence and spread of escaping mutations severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has significantly challenged our efforts in fighting against the COVID-19 pandemic. A broadly neutralizing reagent these concerning variants is thus highly desirable for prophylactic therapeutic treatments SARS-CoV-2 infection. We herein report a covalent engineering strategy on protein minibinders potent neutralization such as B.1.617.2 (Delta), B.1.617.1 (Kappa), B.1.1.529...
Puromycin is an amino-acyl transfer RNA analog widely employed in studies of protein synthesis. Since puromycin covalently incorporated into nascent polypeptide chains, anti-puromycin immunofluorescence enables visualization A common assumption local messenger translation that the staining puromycylated polypeptides fixed cells accurately reports on their original site translation, particularly when ribosomes are stalled with elongation inhibitors prior to treatment. However, we attempted...
The increases in persistent sodium currents (I (Na.P)) and Na(+)/H(+) exchange (NHE) causes intracellular Ca(2+) overload. objective of this study was to determine the contribution I (Na.P) NHE on hypoxia- or acute ischemia-induced increase reverse Na(+)/Ca(2+) current (HIR- AIR-I (NCX)). (NCX) rabbit ventricular myocytes were recorded during hypoxia ischemia, combination acidosis (pH values 6.0 intracellularly 6.8 extracellularly) hypoxia, using whole-cell patch-clamp techniques. results...
Obesity is a growing global health concern associated with severe metabolic disorders, necessitating the development of safer and more effective therapeutic strategies. Soybean sprout peptides (SSPs), derived from germinated soybeans, are bioactive compounds potential antiobesity effects. This study aimed to investigate molecular mechanisms SSPs through an integrated approach combining network pharmacology, docking, in vivo experiments. SSP sequences were identified using...
Background/Aims Resveratrol has been demonstrated to be protective in the cardiovascular system. The aim of this study was assess effects resveratrol on hydrogen peroxide (H2O2)-induced increase late sodium current (INa.L) which augmented reverse Na+-Ca2+ exchanger (INCX), and diastolic intracellular Ca2+ concentration ventricular myocytes. Methods INa.L, INCX, L-type (ICa.L) properties were determined using whole-cell patch-clamp techniques dual-excitation fluorescence photomultiplier...
Antibody-dependent enhancement (ADE) contributes to the pathogenesis of porcine reproductive and respiratory syndrome virus (PRRSV)-persistent infection. However, mechanisms PRRSV-ADE infection are still confusing. A clear understanding event upon by ADE pathway has become crucial for developing efficient intervention PRRSV In this study, an assay showed that in alveolar macrophages (AMs) significantly decreased production interferon-α (IFN-α) tumor necrosis factor-α (TNF-α), increased...
The noncoding RNAs play important role in growth and development of mammalian skeletal muscle. Recent work has shown that circRNAs are abundant muscle tissue, with significant changes their expression patterns during aging. We identified a novel circRNA called circARID1A is highly expressed mice compare to its linear transcript. Experiments significantly inhibited the process C2C12 cell proliferation promoted differentiation. Interactions between miRNA were screened by gene chip sequencing....
Many studies indicate that an increase in late sodium current (I(Na.L)) of cardiomyocytes causes intracellular Na overload and subsequently raises the reverse Na/Ca exchanger (INCX), ultimately resulting Ca overload. Therefore, using drugs to inhibit increased INa.L under various pathological conditions can lower This study was intended explore effect sophocarpine (SOP) on INa.L, INCX, calcium transient contraction rabbit ventricular myocytes induced by Anemonia sulcata toxin II (ATX II),...
The highly expressed circHIPK3 is a circular RNA that has been previously reported to regulate the growth of human cells. In this study, we found an increased expression in bovine mammary epithelial cells treated with prolactin (PRL) high-throughput sequencing data. Thus, further investigated effect on proliferation and differentiation We used qRT-PCR/Cell Counting Kit-8 (CCK-8) Western blotting analysis evaluate effects cell proliferation. promotes STAT5 signaling pathway was associated...
Methylglyoxal (MGO) is a highly reactive metabolite mainly formed as byproduct of glycolysis. Elevated MGO has been considered risk factor for several diseases including diabetes and neurodegeneration. While modifications on proteins were globally profiled, the cross-links between induced by in proteomes are unexplored to date. Here, we reported quantitative chemoproteomic platform based mass shifts that enables identification events protein proteomes. A total 66 cross-linked targets...
Abstract To achieve multisite‐targeting‐based DNA cleavage simultaneously, we designed two kinds of CRISPR RNA arrays by fusing four single guide RNAs (sgRNAs for Cas9 or crRNAs Cpf1) with uncleavable linkers (CRISPRay). The CRISPRay could operate on adjacent target sites to cleave in a collaborative manner. Two demonstrated robust inactivation the firefly luciferase gene living cells. In vitro and sequencing also verified that sgRNA directed SpCas9 nuclease cut at simultaneously whereas...