A5049376781- Mosquito-borne diseases and control
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- SARS-CoV-2 detection and testing
- Viral Infections and Vectors
- Insect symbiosis and bacterial influences
- HIV Research and Treatment
- Malaria Research and Control
- Monoclonal and Polyclonal Antibodies Research
- vaccines and immunoinformatics approaches
- Herpesvirus Infections and Treatments
- Glycosylation and Glycoproteins Research
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- Studies on Chitinases and Chitosanases
- RNA modifications and cancer
- Microfluidic and Capillary Electrophoresis Applications
- CRISPR and Genetic Engineering
- RNA Research and Splicing
- Virology and Viral Diseases
- HIV/AIDS drug development and treatment
- Advanced Biosensing Techniques and Applications
- Microfluidic and Bio-sensing Technologies
- Electrowetting and Microfluidic Technologies
- Viral gastroenteritis research and epidemiology
Colorado State University
2016-2025
University of Colorado Anschutz Medical Campus
2024
Saint Louis University
2000-2023
Bipar
2021
Washington University in St. Louis
2005
ADVERTISEMENT RETURN TO ISSUEPREVReviewNEXTPaper-Based Microfluidic Devices: Emerging Themes and ApplicationsYuanyuan Yang†, Eka Noviana†, Michael P. Nguyen†, Brian J. Geiss‡, David S. Dandy§, Charles Henry*†§View Author Information† Department of Chemistry, Colorado State University, Fort Collins, 80523, United States‡ Microbiology, Immunology, Pathology, States§ Chemical Biological Engineering, States*E-mail: [email protected]. Phone: +1-970-491-2852.Cite this: Anal. Chem. 2017, 89, 1,...
Salmonella causes over a million foodborne illnesses per year in the United States resulting more hospitalizations and deaths than any other bacterial pathogen. To help prevent outbreaks, rapid, portable, sensitive, reliable method for onsite detection of bacteria that can be used different sample matrices would beneficial. Herein, we present colorimetric paper-based analytical device (PAD) combined with immunomagnetic separation (IMS) detecting typhimurium. IMS anti-Salmonella coated...
Coronavirus disease-19 (COVID-19) emerged in late 2019 China and rapidly became pandemic. As with other coronaviruses, a preponderance of evidence suggests the virus originated horseshoe bats ( Rhinolophus spp.) may have infected an intermediate host prior to spillover into humans. A significant concern is that SARS-CoV-2 could become established secondary reservoir hosts outside Asia. To assess this potential, we challenged deer mice Peromyscus maniculatus ) found robust replication upper...
Previous studies have suggested that alpha-glucosidase inhibitors such as castanospermine and deoxynojirimycin inhibit dengue virus type 1 infection by disrupting the folding of structural proteins prM E, a step crucial to viral secretion. We extend these evaluating inhibitory activity against panel clinically important flaviviruses including all four serotypes virus, yellow fever West Nile virus. Using in vitro assays we demonstrated infections were inhibited castanospermine. In contrast,...
The 5′-end of the flavivirus genome harbors a methylated m7 GpppA 2′OMe cap structure, which is generated by virus-encoded RNA triphosphatase, (guanine-N7) methyltransferase, nucleoside 2′- O -methyltransferase, and guanylyltransferase. presence guanylyltransferase activity in NS5 has been suggested several groups but not empirically proven. Here we provide evidence that N-terminus protein true We demonstrate GTP can be used as substrate enzyme to form covalent GMP–enzyme intermediate via...
Abstract Background Arthropod-borne viruses (arboviruses) can persistently infect and cause limited damage to mosquito vectors. RNA interference (RNAi) is a antiviral response important in restricting virus replication has been shown be active against some arboviruses. The goal of this study was use recombinant Sindbis (SINV; family Togaviridae ; genus Alphavirus ) that expresses B2 protein Flock House (FHV; Nodaviridae Alphanodavirus ), inhibits RNAi, determine the effects linking arbovirus...
Abstract Antimicrobial resistance (AMR), the ability of a bacterial species to resist action an antimicrobial drug, has been on rise due widespread use agents. Per World Health Organization, AMR estimated annual cost USD 34 billion in US and is predicted be number one cause death worldwide by 2050. One way bacteria can spread, which individuals contract infections, through contaminated water. Monitoring environment currently requires that samples transported central laboratory for slow labor...
We present a novel partner-specific protein-protein interaction site prediction method called PAIRpred. Unlike most existing machine learning binding methods, PAIRpred uses information from both proteins in protein complex to predict pairs of interacting residues the two proteins. captures sequence and structure about residue through pairwise kernels that are used for training support vector classifier. As result, presents more detailed model binding, offers state art accuracy predicting...
Infectious diseases commonly occur in contaminated water, food, and bodily fluids spread rapidly, resulting death of humans animals worldwide. Among infectious agents, viruses pose a serious threat to public health global economy because they are often difficult detect their infections hard treat. Since it is crucial develop rapid, accurate, cost-effective, in-situ methods for early detection viruses, variety sensors have been reported so far. This review provides an overview the recent...
Zika virus (ZIKV; family Flaviviridae, genus Flavivirus) is a rapidly expanding global pathogen that has been associated with severe clinical manifestations, including devastating neurological disease in infants. There are currently no molecular clones of New World ZIKV available lack significant attenuation, hindering progress toward understanding determinants transmission and pathogenesis. Here we report the development characterization novel reverse genetics system based on 2015 isolate...
Rapid and inexpensive serological tests for SARS-CoV-2 antibodies are needed to conduct population-level seroprevalence surveillance studies can improve diagnostic reliability when used in combination with viral tests. Here, we report a novel low-cost electrochemical capillary-flow device quantify IgG targeting nucleocapsid proteins (anti-N antibody) down 5 ng/mL low-volume (10 μL) human whole blood samples under 20 min. No sample preparation is as the integrates blood-filtration membrane...
Point-of-care (POC) methods currently available for detecting SARS-CoV-2 infections still lack accuracy. Here, we report the development of a highly sensitive electrochemical immunoassay capable quantitatively presence virus in patient nasopharyngeal samples using stencil-printed carbon electrodes (SPCEs) functionalized with capture antibodies targeting nucleocapsid protein (N protein). Samples are added to electrode surface, followed by horseradish peroxidase (HRP)-conjugated detection also...
Viral pathogens are a serious health threat around the world, particularly in resource limited settings, where current sensing approaches often insufficient and slow, compounding spread burden of these pathogens. Here, we describe label-free, point-of-care approach toward detection virus particles, based on microfluidic paper-based analytical device with integrated microwire Au electrodes. The is initially characterized through capturing streptavidin modified nanoparticles by biotin-modified...
The 5' RNA cap structure (m7GpppRNA) is a key feature of eukaryotic mRNAs with important roles in stability, splicing, polyadenylation, mRNA export, and translation. Higher eukaryotes can further modify this minimal the addition methyl group on ribose 2'-O position first transcribed nucleotide (m7GpppNmpRNA) sometimes adjoining (m7GpppNmpNmpRNA). In higher eukaryotes, DXO protein was previously shown to be responsible for both decapping degradation transcripts harboring aberrant ends such as...
Abstract Coronavirus disease-19 (COVID-19) emerged in November, 2019 China and rapidly became pandemic. As with other coronaviruses, a preponderance of evidence suggests the virus originated horseshoe bats ( Rhinolophus spp.) likely underwent recombination event an intermediate host prior to entry into human populations. A significant concern is that SARS-CoV-2 could become established secondary reservoir hosts outside Asia. To assess this potential, we challenged deer mice Peromyscus...
We present novel flow control methods including valve, mixing control, and rate applicable to the laminate capillary-driven microfluidic devices.
Abstract The COVID-19 pandemic underlines the need for effective strategies controlling virus spread and ensuring sensitive detection of SARS-CoV-2. This review presents potential nanomaterial-enabled optical biosensors rapid low-cost SARS-CoV-2 biomarkers, demonstrating a comprehensive analysis including colorimetric, fluorescence, surface-enhanced Raman scattering, surface plasmon resonance methods. Nanomaterials metal-based nanomaterials, metal–organic frame–based nanoparticles, nanorods,...
Arthropod-borne flavivirus infection causes serious morbidity and mortality worldwide, but there are currently no effective antiflaviviral chemotherapeutics available for human use. Therefore, it is critical that new therapeutics against virus-specific targets be developed. To identify compounds may used as broadly active therapeutics, we have performed a high-throughput screening of 235,456 commercially small-molecule inhibitors the dengue virus NS5 RNA capping enzyme. We identified family...