A5011450118- vaccines and immunoinformatics approaches
- Monoclonal and Polyclonal Antibodies Research
- SARS-CoV-2 detection and testing
- Glycosylation and Glycoproteins Research
- Biosensors and Analytical Detection
- Advanced biosensing and bioanalysis techniques
- RNA and protein synthesis mechanisms
- SARS-CoV-2 and COVID-19 Research
- Innovative Microfluidic and Catalytic Techniques Innovation
- Viral gastroenteritis research and epidemiology
Colorado State University
2020-2025
University of Colorado Anschutz Medical Campus
2024
Point-of-care (POC) methods currently available for detecting SARS-CoV-2 infections still lack accuracy. Here, we report the development of a highly sensitive electrochemical immunoassay capable quantitatively presence virus in patient nasopharyngeal samples using stencil-printed carbon electrodes (SPCEs) functionalized with capture antibodies targeting nucleocapsid protein (N protein). Samples are added to electrode surface, followed by horseradish peroxidase (HRP)-conjugated detection also...
Defining the binding epitopes of antibodies is essential for understanding how they bind to their antigens and perform molecular functions. However, while determining linear monoclonal can be accomplished utilizing well-established empirical procedures, these approaches are generally labor- time-intensive costly. To take advantage recent advances in protein structure prediction algorithms available scientific community, we developed a calculation pipeline based on localColabFold...
A capillary-driven microfluidic sequential flow device, designed for eventual at-home or doctor's office use, was developed to perform an enzyme-linked immunosorbent assay (ELISA) serology assays.
Defining the binding epitopes of antibodies is essential for understanding how they bind to their antigens and perform molecular functions. However, while determining linear monoclonal can be accomplished utilizing well-established empirical procedures, these approaches are generally labor-and time-intensive costly. To take advantage recent advances in protein structure prediction algorithms available scientific community, we developed a calculation pipeline based on localColabFold...
Abstract The global COVID-19 pandemic has caused massive disruptions in every society around the world. To help fight COVID-19, new molecular tools specifically targeting critical components of causative agent SARS-Coronavirus-2 (SARS-CoV-2), are desperately needed. SARS-CoV-2 nucleocapsid protein is a major component viral replication processes, integral to particle assembly, and diagnostic marker for infection immune protection. Currently available antibody reagents were primarily...
Defining the binding epitopes of antibodies is essential for understanding how they bind to their antigens and perform molecular functions. However, while determining linear monoclonal can be accomplished utilizing well-established empirical procedures, these approaches are generally labor-and time-intensive costly. To take advantage recent advances in protein structure prediction algorithms available scientific community, we developed a calculation pipeline based on localColabFold...
Defining the binding epitopes of antibodies is essential for understanding how they bind to their antigens and perform molecular functions. However, while determining linear monoclonal can be accomplished utilizing well-established empirical procedures, these approaches are generally labor- time-intensive costly. To take advantage recent advances in protein structure prediction algorithms available scientific community, we developed a calculation pipeline based on localColabFold...
A disposable enzyme-linked immunosorbent assay (dELISA) device for ate-home or doctor’s office use was developed to detect SARS-CoV-2 antibodies. Serology testing antibodies is currently run using well-plate ELISAs in centralized laboratories. However, the scale of serology needed epidemiological and clinical screening studies will overwhelm existing laboratory resources. Instead, a point-of-need that can be used at home offices COVID-19 must one four target products prioritized by World...