A5024683104- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Virology and Viral Diseases
- Identification and Quantification in Food
- Viral Infections and Immunology Research
- Herpesvirus Infections and Treatments
- Poxvirus research and outbreaks
- Probiotics and Fermented Foods
- Food Safety and Hygiene
- Listeria monocytogenes in Food Safety
- Virus-based gene therapy research
- Enterobacteriaceae and Cronobacter Research
- COVID-19 Clinical Research Studies
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- SARS-CoV-2 and COVID-19 Research
- Essential Oils and Antimicrobial Activity
- Malaria Research and Control
- Vector-borne infectious diseases
- Aquaculture disease management and microbiota
- Mosquito-borne diseases and control
- COVID-19 epidemiological studies
- Vibrio bacteria research studies
- Antibiotic Resistance in Bacteria
University of Tripoli
2016-2025
The Pirbright Institute
2006-2024
Center for Solar Energy Research and Studies
2024
Little is understood about the immune responses involved in pathogenesis of infectious bursal disease virus (IBDV). Strains IBDV differ their virulence: F52/70 a classical virulent strain (vIBDV), whereas UK661 very (vvIBDV) that causes greater pathology and earlier mortality. The exact clinical death are still unclear. Pro-inflammatory cytokines such as interleukin (IL)-1β IL-6, produced by activated macrophages, could play role, T natural killer (NK) cells, interferon (IFN)-γ, which...
Foot-and-mouth disease (FMD) is a highly contagious of livestock affecting animal production and trade throughout Asia Africa. Understanding FMD virus (FMDV) global movements evolution can help to reconstruct the spread between endemic regions predict risks incursion into FMD-free countries. Global expansion single FMDV lineage rare but result in severe economic consequences. Using extensive sequence data we have reconstructed space-time transmission history O/ME-SA/Ind-2001 (which normally...
Foot-and-mouth disease viruses are often restricted to specific geographical regions and spread new areas may lead significant epidemics. Phylogenetic analysis of sequences the VP1 genome region recent outbreak from Libya Saudi Arabia has revealed a lineage, O-Ind-2001, normally found in Indian subcontinent. This paper describes characterization field collected these cases provides information about real-time RT-PCR assay that can be used detect this lineage discriminate them other endemic...
On March 2013, the Libyan poultry industry faced severe outbreaks due to mixed infections of APMV-1 (Newcastle disease) and low pathogenic avian influenza (AI) H9N2 subtype which were causing high mortality great economic losses. isolated characterized. Genetic sequencing APMV-1/chicken/Libya/13VIR/ 7225-1/2013 isolate revealed presence a velogenic belonging lineage 5 (GRRRQKR*F Lin.5) or genotype VII in class II, according nomenclature use. Three AI viruses subtype, namely...
Aim: The aim of this work was to isolate and molecularly identify enterohemorrhagic Escherichia coli (EHEC) O157 in milk dairy products Libya, addition; clear the accuracy cultural biochemical identification as compared with molecular by partial sequencing 16S rDNA for existing isolates.Materials Methods: A total 108 samples raw (cow, she-camel, goat) locally made (fermented cow's milk, Maasora, Ricotta ice cream) were collected from some regions (Janzour, Tripoli, Kremiya, Tajoura Tobruk)...
The aim of the current investigation was to screen presence Staphylococci spp., especially S. aureus in meat, meat products different animal species, and some seafood sold retail markets Libya using cultural molecular techniques, study their antibiotics resistance profiles.A total 139 samples from red products, were collected many areas Libya. Enumeration isolation spp. by normal methods followed identification techniques bacterial DNA extraction partial sequencing 16S rDNA.Out samples, 112...
Antibiotic resistance represents a fundamental issue affecting public health today. occurs when bacteria alter their response to antibiotics. Pathogenic and genes can diffuse throughout human natural habitats. The complicated interactions between diverse bacterial communities that affect the of people, aquatic animals, environment are an illustration ecological issues. Pollution oceans by antibiotic-resistant (ARB) disturb equilibrium may spread humans. Also, climate change (CC)...
The genus Vibrio includes several food-borne pathogens that cause a spectrum of clinical conditions including septicemia, cholera and milder forms gastroenteritis. Several spp. are commonly associated with transmission cholerae, parahemolyticus, vulnificus. Microbiological analysis for enumeration isolation were carried out total 93 samples seafood, meat products from different geographic localities in Libya (Tripoli, Regdalin, Janzour Tobruk). detected by conventional cultural molecular...
Sporadic outbreaks of foot-and-mouth disease (FMD) have occurred in Libya for almost fifty years. During the spring 2013, a countrywide serosurvey was undertaken to assess level FMD virus circulation and identify serotypes country. A total 4221 sera were collected, comprising samples from large ruminants (LR; n=1428 357 farms) small (SR; n=2793 141 farms). sero-prevalence NSP antibodies determined by ELISA 19.0% (271/1428) with 95% CI (16.9 - 21.0) 13.5% (378/2793) (12.3 14.8) LR SR samples,...
North Africa has a great diversity of indigenous sheep breeds whose origin is linked to its environmental characteristics and certain historical events that took place in the region. To date, few genome-wide studies have been conducted investigate population structure African sheep. The objective present study was provide detailed assessment genetic admixture patterns six Maghreb populations using Illumina 50K Ovine BeadChip comparisons with 22 global mouflon. Regardless method analysis...
This study aimed to evaluate the antibacterial activity of flavonoids extracted from two Libyan brown algae namely Cystoseira compressa and Padina pavonica using microwave-assisted extraction method against pathogenic bacteria isolated meat, meat products, milk dairy products (Staphylococcus aureus subsp. (5 isolates), Bacillus cereus (3 pumilus (1 isolate), Salmonella enterica enteric (4 isolates) Enterohaemor-rhagic Escherichia coli O157 (EHEC O157) isolates)). All these isolates were...
Foot-and-mouth disease (FMD) affects the livestock industry and socioeconomic sustainability of many African countries. The success FMD control programs in Africa depends largely on understanding dynamics virus (FMDV) spread. In light recent outbreaks that affected North-Western countries 2018 2019, we investigated evolutionary phylodynamics causative serotype O viral strains all belonging to East-Africa 3 topotype (O/EA-3). We analyzed a total 489 sequences encoding FMDV VP1 genome region...
West Nile virus (WNV) is a globally significant mosquito-borne Flavivirus that causes disease (WND). In Libya, evidence of WNV circulation has been reported in humans but never animals. The aim this study was to determine the seroprevalence infection horses and dogs Libya. total, 574 63 serum samples were collected from apparently healthy, unvaccinated dogs, respectively, between 2016 2019. A commercially available competitive enzyme-linked immunosorbent assay (c-ELISA) kit initially used...
Abstract The epidemiological patterns of Bluetongue ( BT ) in North Africa and Mediterranean Basin MB dramatically changed by emergence subsequent episodes novel bluetongue virus BTV serotypes with highly pathogenic indexes socio‐economic impacts. objective the study was to investigate sero‐prevalence serotype distribution Libya. During 2015‐2016, a total 826 serum samples were collected from domestic ruminants All sera assayed competitive enzyme‐linked immunosorbent assays (c‐ ELISA )....
A cross‑sectional study was conducted in Libya 7 areas of Tripoli to determine the seroprevalence Peste des Petits Ruminants (PPR) Virus (PPRV) small ruminants (sheep and goats) between June August 2013, identify potential risk factors associated with infection. The involved 10% ruminant herds ≥50 animals region. They were selected randomly (15 herds), 35 58 samples, depending on its size, collected from each herd. Seven‑hundred twenty‑one serum samples unvaccinated (601 sheep 120 then...
Background and Aim: Foodborne illnesses are a serious challenge to human health the economic sector. For example, salmonellosis remains burden in developed developing nations. Rapid reliable molecular methods identify Salmonella strains essential for minimizing infection. This study aimed spp. raw milk dairy products using conventional techniques test antibiotic susceptibility of isolated strains. Materials Methods: One hundred thirty-one product samples were randomly collected from...
We conducted a cross-sectional study during 2013 to quantify the serological prevalence of peste des petits ruminants (PPR) infection and investigate host factors associated with PPR in small Libya. A two-stage sampling design was carried out. total number 148 flocks owning at least 100 heads each were randomly selected. Sixteen forty-eight samples collected from selected flock. 3,508 serum unvaccinated animals analysed IZSLER Brescia, Italy, by using competitive ELISA, IDvet innovative...
Foot-and-mouth disease (FMD) is a viral disease, widespread and highly contagious, that mainly affects cloven-hoofed domestic wild animals. FMD can lead to high economic losses due the reduction in animal production such as drop milk production, loss of body weight, mortality rate young ruminants. Sixteen samples were collected from animals showing typical clinical signs during last outbreak Libya 2018-2019. Flinders Technology Associates (FTA) cards impressed with blood, swabs, or vesicular...
Background: Cronobacter species is the most significant foodborne pathogens in infant milk formula (IMF). These have been incriminated sever form of neonatal meningitis, sepsis and necrotizing enterocolitis with high mortality rate. Aim: The current study was performed to demonstrate effect heat stress on spp. (C. sakazakii C. pulveris) reconstituted IMF (RIMF). Methods: inoculated five isolates four pulveris separately. nine were heated RIMF at 48, 52, 56, 60, 64 66°C. D- z-values...
In mammals, the role of interleukin-18 (IL-18) in immune response is to drive inflammatory and, normally therefore, anti-viral responses. IL-18 also shows promise as a vaccine adjuvant mammals. Chicken (chIL-18) has been cloned. The aim this study was investigate potential chIL-18 act context live recombinant Fowlpox virus (fpIBD1) against Infectious bursal disease (IBDV). fpIBD1 protects mortality, but not damage bursa Fabricius caused by IBDV infection. genome itself contains several...
Background: Food poisoning caused by bacterial agents is a worldwide problem, usually accompanied unpleasant symptoms and may be sever leading to death. Natural compounds from marine algae namely flavonoids play role in the remedy of this condition. Aim: This research aims assess potency extracted Enteromorpha intestinalis Caulerpa prolifera as antibacterial agents. Methods: E. was collected Western Libyan Coast C. Farwa Island. The antimicrobial activity determination minimum inhibitory...
Background and Aim: Klebsiella pneumoniae is one of the most common causes clinical asymptomatic mastitis in dairy cattle, as well milk products that affect quality. Mastitis caused by K. even more serious due to its poor response antibiotic therapy. The aim this study was detect identify presence produced Libya. Materials Methods: A total 234 samples were randomly collected from various locations Samples examined for using conventional cultural techniques, including cultivation violet red...
The bioactive algal components that contribute to their antimicrobial properties have garnered significant attention, particularly in light of the rising incidence antibiotic resistance. current study aimed evaluate antibacterial activity a Petalonia fascia methanolic extract and explore potentiation action extract–antibiotic combinations with available antibiotics, as well effects mixture on multidrug-resistant (MDR) bacteria, namely, five methicillin-resistant Staphylococcus aureus (MRSA)...