Abstract Lumpy skin disease (LSD) is a devastating of cattle characterized by fever, nodules on the skin, lymphadenopathy and milk drop. Several haematophagous arthropod species like dipterans ticks are suspected to play role in transmission LSDV. Few conclusive data however available importance biting flies horseflies as potential vectors LSDV transmission. Therefore an vivo study was carried out investigate possible Stomoxys calcitrans Haematopota spp . from experimentally infected...

Vaccines form the cornerstone of any control, eradication and preventative strategy this is no different for lumpy skin disease. However, usefulness a vaccine determined by multiplicity factors which include stability, efficiency, safety ease use, to name few. Although vaccination campaign in Balkans against disease virus (LSDV) was successful has been implemented with success past other countries, data failure have also reported. It therefore purpose study compare five homologous live...

From 2017 to 2019, several vaccine-like recombinant strains of lumpy skin disease virus (LSDV) were discovered in Kazakhstan and neighbouring regions Russia China. Shortly before their emergence, the authorities launched a mass vaccination campaign with Neethling-based Lumpivax vaccine. Since none other countries affected region had used homologous LSDV vaccine, it was soon suspected that vaccine cause these unusual strains. In this study, we performed genome-wide molecular analysis...

Lumpy skin disease virus (LSDV) is a vector-transmitted capripox that causes in cattle. Stomoxys calcitrans flies are considered to be important vectors as they able transmit viruses from cattle with the typical LSDV nodules naive No conclusive data are, however, available concerning role of subclinically or preclinically infected transmission. Therefore, an vivo transmission study 13 donors, experimentally inoculated LSDV, and naïve acceptor bulls was performed whereby S. were fed on either...

Lumpy skin disease is a high-consequence in cattle caused by infection with the poxvirus lumpy virus (LSDV). The endemic most countries Africa and an emerging threat to populations Europe Asia. As LSDV spreads into new regions, it important that signs of are recognized promptly animal caregivers. This study describes gross, microscopic, ultrastructural changes occur over time experimentally challenged LSDV. Four calves were inoculated wildtype monitored for 19 21 days. At 7 days after...

Lumpy Skin Disease virus is a poxvirus from the genus Capripox that mainly affects bovines and it causes severe economic losses to livestock holders. The currently dispersing in Asia, but little known about detailed phylogenetic relations between strains genome evolution. We reconstructed whole-genome-sequence (WGS)-based phylogeny compared with single-gene-based phylogenies. To study population spatiotemporal patterns greater detail, we networks. determined there are multiple clades within...

ABSTRACT Since 1998, 9 of the 26 serotypes bluetongue virus (BTV) have spread throughout Europe, and serotype 8 has suddenly emerged in northern causing considerable economic losses, direct (mortality morbidity) but also indirect, due to restriction animal movements. Therefore, many new types vaccines, particularly subunit with improved safety efficacy for a broad range BTV are currently being developed by different laboratories. Here we exploited reverse genetics-based replication-deficient...

The aim of this study was to evaluate the general characteristics commercially available enzyme-linked immunosorbent assays (ELISAs) detect antibody against classical swine fever (CSF), as well assess their potential use accompanying marker tests able differentiate infected from vaccinated animals (DIVA). Chekit* CSF-Sero and HerdChek* CSFV Ab, both which antibodies E2 protein virus (CSFV), had highest sensitivity. Both were practicable showed good reproducibility. Comparable sensitivity...

Lumpy skin disease virus (LSDV) causes an economically important in cattle. Here, we report the complete genome sequence of first LSDV isolate identified mainland Europe. Evros/GR/15 was isolated from cases reported on 18 August 2015 Evros region, Greece.

During this study a new Immunoperoxidase Monolayer Assay (IPMA) was developed for the detection of antibodies against lumpy skin disease virus (LSDV) in an easy and low tech setting. Using two dilutions (1:50 1:300) duplicate format, test shown to be highly sensitive, specific repeatable. In comparison VNT commercial ELISA, LSDV-IPMA able detect LSDV earlier infected, vaccinated vaccinated/infected animals. The assay is very flexible as it can easily adapted sheeppox or goatpox scaled-up...

Vaccination is an effective approach to prevent, control and eradicate diseases, including lumpy skin disease (LSD). One of the measures address farmer hesitation vaccinate guaranteeing quality vaccine batches. The purpose this study was demonstrate importance a procedure via evaluation LSD vaccine, Lumpivax (Kevevapi). initial PCR screening revealed presence wild type virus (LSDV) goatpox (GTPV), in addition LSDV. New phylogenetic PCRs were developed characterize detail genomic content...

The current epidemic in Asia, driven by LSDV recombinants, poses difficulties to existing DIVA PCR tests, as these do not differentiate between homologous vaccine strains and the recombinant strains. We, therefore, developed validated a new duplex real-time capable of differentiating Neethling-based from classical wild-type that are currently circulating Asia. potential this assay, seen silico evaluation, was confirmed on samples infected vaccinated animals isolates recombinants (n = 12),...

ABSTRACT Lumpy skin disease virus (LSDV) causes an economically important in cattle. Here, we report the complete genome sequences of three LSDV strains obtained directly from live attenuated vaccines: Lumpyvax (MSD Animal Health), Herbivac LS (Deltamune) and Skin Disease Vaccine (Onderstepoort Biological Products).

Vaccines have proven themselves as an efficient way to control and eradicate lumpy skin disease (LSD). In addition the safety efficacy aspects, it is important know duration for which vaccines confer protective immunity, this impacts design of eradication program. We evaluated immunity induced by a live attenuated vaccine (LSDV LAV) inactivated Inac), both based on LSDV. Cattle were vaccinated challenged after 6, 12 18 months LSDV LAV or 6 Inac. The elicited strong immune response protection...

ABSTRACT Lumpy skin disease virus (LSDV) causes a nodular dermatitis in cattle and has high economic consequences affected areas. Detection of LSDV exposure mostly relies on the humoral immune response, while cell-mediated (CMI) an important hallmark reaction to LSDV, is neglected. We collected samples during 3 weeks post-vaccination with Neethling-based live attenuated vaccine (LAV) 4 post-LSDV infection under experimental conditions i) investigate development CMI ii) optimize...

Vaccination is the main control measure to prevent Lumpy skin disease (LSD), and Neethling-based homologous vaccines have been shown be safe effective against infection with classical clade 1.2 strains. In 2017, recombinant 2 LSDV strains originating from a badly produced insufficiently controlled vaccine were first detected in Russia. A 2.5 strain spread Russia throughout Southeast Asia caused massive epidemic. this study, efficacy of three different Neethling strain-based was evaluated....

Sheep pox is endemic in most parts of Northern Africa and has the potential to cause severe economic problems. Live attenuated vaccines are used Morocco, many other countries, control disease. virus (SPPV) re-appeared 2010 causing a nodular clinical form previously not observed Morocco. The signs during course this outbreak initial reports citing similarity nucleotide sequence between Moroccan vaccine strain field isolates warranted more depth analysis epizootic. In study, showed that...

Bluetongue virus (BTV) is endemic in many parts of the world, often causing severe hemorrhagic disease livestock. To date, at least 27 different serotypes have been recognized. Vaccination against all necessary to protect susceptible animals and prevent onward spread by insect vectors. In our previous studies, we generated replication-deficient (disabled infectious single-cycle [DISC]) strains for a number reported preliminary data on their protective efficacy animals. this report, advance...

Capripoxviruses cause economically important diseases in domestic ruminants regions endemic for these viruses. We report here the complete genome sequence of KSGP 0240 vaccine strain from live attenuated Kenyavac (JOVAC).

Lumpy skin disease (LSD) diagnosis is primarily based on clinical surveillance complemented by PCR of lesion crusts or nodule biopsies. Since LSD can be subclinical, the sensitivity could lower than expected. Furthermore, real-time for detection viral DNA in blood samples from subclinical animals only intermittently positive. Therefore, this study aimed to investigate an acceptable, easily applicable and more sensitive testing method LSD. An animal experiment was conducted ear notches...

Lumpy skin disease virus (LSDV) causes a severe, systemic, and economically important in cattle. Here, we report coding-complete sequences of recombinant LSDVs from four outbreaks October November 2020 northeastern Vietnam.