A5015847011- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Infectious Diseases and Tuberculosis
- Diagnosis and treatment of tuberculosis
- Pneumonia and Respiratory Infections
- Vibrio bacteria research studies
- Aquaculture disease management and microbiota
- Salmonella and Campylobacter epidemiology
- Pneumocystis jirovecii pneumonia detection and treatment
- Disaster Response and Management
- Genomics and Rare Diseases
- Biochemical and Molecular Research
- Bacteriophages and microbial interactions
- Antibiotic Resistance in Bacteria
- COVID-19 and healthcare impacts
- Gene expression and cancer classification
- Bacterial biofilms and quorum sensing
- Hepatitis C virus research
- Orthopedic Infections and Treatments
- Biosimilars and Bioanalytical Methods
- Genetics and Neurodevelopmental Disorders
- Biomedical Text Mining and Ontologies
- Escherichia coli research studies
- Bacterial Genetics and Biotechnology
- Epilepsy research and treatment
National Center for HIV/AIDS Viral Hepatitis STD and TB Prevention
2011-2022
Centers for Disease Control and Prevention
2012-2022
University of Colorado Denver
2022
Children's Hospital Colorado
2022
Epidemic Intelligence Service
2021
Florida College
2006
University of Florida
2000-2006
Emory University
2005
The emergence of multi- and extensively drug-resistant tuberculosis is a significant impediment to the control this disease because treatment becomes more complex costly. Reliable timely drug susceptibility testing critical ensure that patients receive effective become noninfectious. Molecular methods can provide accurate rapid results. We used DNA sequencing detect resistance first-line antituberculosis drugs isoniazid (INH), rifampin (RIF), pyrazinamide (PZA), ethambutol (EMB) second-line...
A clear understanding of the genetic basis antibiotic resistance in Mycobacterium tuberculosis is required to accelerate development rapid drug susceptibility testing methods based on sequence. Raw genotype–phenotype correlation data were extracted as part a comprehensive systematic review develop standardised analytical approach for interpreting associated mutations rifampicin, isoniazid, ofloxacin/levofloxacin, moxifloxacin, amikacin, kanamycin, capreomycin, streptomycin,...
On May 5, 2021, CDC's Tuberculosis Trials Consortium and the National Institutes of Health (NIH)-sponsored AIDS Clinical Group (ACTG) published results from a randomized controlled trial indicating that 4-month regimen containing rifapentine (RPT), moxifloxacin (MOX), isoniazid (INH), pyrazinamide (PZA) was as effective standard 6-month for tuberculosis (TB) treatment (1).On basis these findings, CDC recommends option U.S. patients aged ≥12 years with drug-susceptible pulmonary TB provides...
Drug-resistant tuberculosis poses a persistent public health threat. The ReSeqTB platform is collaborative, curated knowledgebase, designed to standardize and aggregate global Mycobacterium complex (MTBC) variant data from whole genome sequencing (WGS) with phenotypic drug susceptibility testing (DST) clinical data. We developed unified analysis pipeline (UVP) ( https://github.com/CPTR-ReSeqTB/UVP ) identify variants assign lineage MTBC sequence Stringent thresholds quality control measures...
It is currently unknown how often and in which ways a genetic diagnosis given to patient with epilepsy associated clinical management outcomes. To evaluate diagnoses patients are This was retrospective cross-sectional study of referred for multigene panel testing between March 18, 2016, August 3, 2020, outcomes reported May November 2020. The setting included commercial laboratory multicenter practices. Patients epilepsy, regardless sociodemographic features, who received pathogenic/likely...
During July 7-11, 2023, CDC received reports of two patients in different states with a tuberculosis (TB) diagnosis following spinal surgical procedures that used bone allografts containing live cells from the same deceased donor. An outbreak associated similar product manufactured by tissue establishment (i.e., manufacturer) occurred 2021. Because concern these cases represented second outbreak, and Food Drug Administration worked to determine this was obtained donor one implicated 2021...
Continued progress in addressing challenges associated with detection and management of tuberculosis requires new diagnostic tools. These tools must be able to provide rapid accurate information for detecting resistance guide selection the treatment regimen each patient. To achieve this goal, globally representative genotypic, phenotypic, clinical data are needed a standardized curated platform. A global partnership academic institutions, public health agencies, nongovernmental organizations...
ABSTRACT Ethambutol resistance in clinical Mycobacterium tuberculosis isolates is associated primarily with missense mutations the embB gene. However, recent reports have described presence of mutations, especially those at codon 306, susceptible to ethambutol. To clarify role ethambutol resistance, we sequenced resistance-determining region spontaneous ethambutol-resistant mutants. In our study, 66% mutants contained a single point mutation , 55% these occurring 306. The MIC for was...
ABSTRACT Vibrio vulnificus is an opportunistic pathogen that contaminates oysters harvested from the Gulf of Mexico. In humans with compromising conditions, especially excess levels iron in plasma and tissues, consumption contaminated seafood or exposure wounds to water can lead systemic infection disfiguring skin extremely high mortality. V. -associated diseases are noted for rapid replication bacteria host extensive tissue damage. this study we examined virulence attributes three virulent...
ABSTRACT Vibrio vulnificus is an opportunistic gram-negative pathogen that commonly contaminates oysters. Predisposed individuals who consume raw oysters can die within days from sepsis, and even otherwise healthy people are susceptible to serious wound infection after contact with contaminated seafood or seawater. Numerous secreted cell-associated virulence factors have been proposed account for the fulminating destructive nature of V. infections. Among putative elastolytic metalloprotease....
Summary Objectives Broth‐based culture (BBC) systems are increasingly being used to detect Mycobacterium tuberculosis complex (MTBC) in resource‐limited. We evaluated the performance, time detection and cost of Capilia TB identification test from broth cultures positive for acid‐fast bacilli (AFB) Thailand. Methods From October–December 2007, that grew AFB specimens submitted by district clinics Bangkok city laboratory were tested MTBC using standard biochemical tests. Isolates identified as...
ABSTRACT Multidrug-resistant (MDR) isolates of Mycobacterium tuberculosis complex (MTBC) are defined by resistance to at least rifampin (RMP) and isoniazid (INH). Rapid accurate detection multidrug is essential for effective treatment interruption disease transmission (TB). Overdiagnosis MDR TB may result in with second-line drugs that more costly, less effective, poorly tolerated than first-line drugs. CDC offers rapid confirmation the molecular drug (MDDR) mutations associated RMP INH...
We conducted a multicenter study to determine whether Mycobacterium tuberculosis complex (MTBC) cultures in automated broth-based systems could reliably be considered negative sooner than 6 weeks. Laboratory sites used Bactec MGIT or BacT/Alert and tracked results of time detection all mycobacteria (TTD-all, n = 1547) MTBC (TTD-MTBC, 466) over 6-month periods from primarily (93%) respiratory specimens. Cumulative percentages by day detected median TTD initial follow-up specimens were...
Since 1953, through the cooperation of state and local health departments, U.S. Centers for Disease Control Prevention (CDC) has collected information on incident cases tuberculosis (TB) disease in United States. In 2009, TB case rates declined -11.4%, compared to an average annual -3.8% decline since 2000. The unexpectedly large raised concerns that may have gone unreported. To address unexpected decline, we examined trends from multiple sources treatment initiation, medication sales,...
Ethambutol (EMB) is used as a part of drug regimens for treatment tuberculosis (TB). Susceptibility Mycobacterium complex (MTBC) isolates to EMB can be discerned by DNA sequencing detect mutations in the embB gene associated with resistance. US Public Health Laboratories (PHL) primarily use growth-based susceptibility test (DST) methods determine The Centers Disease Control and Prevention (CDC) provides service molecular detection resistance (MDDR) concurrent DST using agar proportion. PHL...
Objectives. To assess SARS-CoV-2 transmission within a correctional facility and recommend mitigation strategies.Methods. From April 29 to May 15, 2020, we established the point prevalence of COVID-19 among incarcerated persons staff in Arkansas. Participants provided respiratory specimens for testing completed questionnaires on symptoms factors associated with transmission.Results. Of 1647 128 tested, 30.5% (range by housing unit = 0.0%-58.2%) 2.3% tested positive SARS-CoV-2. Among those...
CDC worked closely with the Puerto Rico Department of Health and other federal, state, nongovernmental partners in providing technical support aid reestablishing laboratory operations during emergency response after Hurricane Maria.
Summary Vibrio vulnificus is Gram‐negative bacterium that contaminates oysters, causing highly lethal sepsis after consumption of raw oysters and wound infection. We previously described two sets V. strains with different levels virulence in subcutaneously inoculated iron dextran‐treated mice. Both virulent, clinical attenuated, environmental could be recovered high numbers from skin lesions livers; however, the attenuated required significantly higher colony‐forming units (cfu) inoculum to...
Abstract Tuberculosis is the primary infectious disease killer worldwide, with a growing threat from multidrug-resistant cases. Unfortunately, classic growth-based phenotypic drug susceptibility testing (DST) remains difficult, costly, and time consuming, while current rapid molecular options are limited by diversity of antimicrobial-resistant genotypes that can be detected at once. Next-generation sequencing (NGS) offers opportunity for rapid, comprehensive DST without or cost burden tests...
CS1 pili are important virulence factors of enterotoxigenic Escherichia coli strains associated with human diarrheal disease. They the prototype for a family that share extensive sequence similarity among their structural and assembly proteins. Only four linked genes, cooB, cooA, cooC, cooD, required to produce in E. K-12. To identify amino acids function major pilin CooA, we used alanine substitution mutagenesis targeting conserved residues N C termini protein. test function, examined cooA...