Jayne S. Sutherland
A5065441409- Tuberculosis Research and Epidemiology
- Mycobacterium research and diagnosis
- Pneumonia and Respiratory Infections
- Infectious Diseases and Tuberculosis
- vaccines and immunoinformatics approaches
- Immunodeficiency and Autoimmune Disorders
- Diagnosis and treatment of tuberculosis
- T-cell and B-cell Immunology
- Pneumocystis jirovecii pneumonia detection and treatment
- Immunotherapy and Immune Responses
- Influenza Virus Research Studies
- Immune responses and vaccinations
- Immune Cell Function and Interaction
- Hematopoietic Stem Cell Transplantation
- Respiratory viral infections research
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- HIV Research and Treatment
- Hematological disorders and diagnostics
- Biochemical and Molecular Research
- COVID-19 Clinical Research Studies
- S100 Proteins and Annexins
- Immune cells in cancer
- Iron Metabolism and Disorders
- Complement system in diseases
- Vaccine Coverage and Hesitancy
MRC Unit the Gambia
2016-2025
Başkent University Hospital
2023
Universitat de Barcelona
2023
Barcelona Institute for Global Health
2023
European Respiratory Society
2023
Swansea Bay University Health Board
2020
Morriston Hospital
2020
Medical Research Council
2012-2019
London School of Hygiene & Tropical Medicine
2019
New York University
2013
Abstract The thymus undergoes age-related atrophy, coincident with increased circulating sex steroids from puberty. impact of thymic atrophy is most profound in clinical conditions that cause a severe loss peripheral T cells the ability to regenerate adequate numbers naive CD4+ indirectly correlating patient age. present study demonstrates androgen ablation results complete regeneration aged male mouse thymus, restoration cell phenotype and function enhanced following bone marrow...
Rationale: Contacts of patients with tuberculosis (TB) constitute an important target population for preventive measures because they are at high risk infection Mycobacterium and progression to disease.Objectives: We investigated biosignatures predictive ability incident TB.Methods: In a case–control study nested within the Grand Challenges 6-74 longitudinal HIV-negative African cohort exposed household contacts, we employed RNA sequencing, PCR, pair ratio algorithm in training/test set...
Abstract Age-associated thymic involution is accompanied by decreased output. This adversely affects general immune competence and T cell recovery following cytoreductive treatments such as chemotherapy. A causal link between increasing sex steroids age-related atrophy well established. Although castration has been demonstrated to regenerate the atrophied thymus, little known about how this initiated or kinetics of thymocyte regeneration. The present study shows that although impacts...
Tuberculosis (TB) remains a global health problem. The solution involves development of an effective vaccine, but has been limited by incomplete understanding what constitutes protective immunity during natural infection with Mycobacterium tuberculosis. In this study, M. tuberculosis-specific responses following overnight whole-blood assay were assessed intracellular cytokine staining and luminex, compared between TB cases exposed household contacts. had significantly higher levels...
<h3>Background</h3> User-friendly, rapid, inexpensive yet accurate TB diagnostic tools are urgently needed at points of care in resource-limited settings. We investigated host biomarkers detected serum samples obtained from adults with signs and symptoms suggestive primary healthcare clinics five African countries (Malawi, Namibia, South Africa, The Gambia Uganda), for the diagnosis disease. <h3>Methods</h3> prospectively enrolled individuals presenting warranting investigation pulmonary TB,...
New biomarkers of tuberculosis (TB) risk and disease are critical for the urgently needed control ongoing TB pandemic. In a prospective multisite study across Subsaharan Africa, we analyzed metabolic profiles in serum plasma from HIV-negative, TB-exposed individuals who either progressed to 3-24 months post-exposure (progressors) or remained healthy (controls). We generated trans-African biosignature TB, which identifies future progressors both on blinded test samples external data sets...
Abstract Improved tuberculosis diagnostics and tools for monitoring treatment response are urgently needed. We developed a robust simple, PCR-based host-blood transcriptomic signature, RISK6, multiple applications: identifying individuals at risk of incident disease, as screening test subclinical or clinical tuberculosis, treatment. RISK6 utility was validated by blind prediction using quantitative real-time (qRT) PCR in seven independent cohorts. Prognostic performance significantly...
Background A nonsputum blood test capable of predicting progression healthy individuals to active tuberculosis (TB) before clinical symptoms manifest would allow targeted treatment curb transmission. We aimed develop a proteomic biomarker risk TB for ultimate translation into point-of-care diagnostic. Methods and findings Proteomic signatures were discovered in longitudinal cohort 6,363 Mycobacterium tuberculosis-infected, HIV-negative South African adolescents aged 12–18 years (68% female)...
The development of a fast and accurate, non-sputum-based point-of-care triage test for tuberculosis (TB) would have major impact on combating the TB burden worldwide. A new fingerstick blood has been developed by Cepheid (the Xpert MTB Host Response [MTB-HR] prototype), which generates "TB score" based messenger RNA (mRNA) expression 3 genes. Here we describe first prospective findings MTB-HR prototype.
The experiences of frontline healthcare professionals are essential in identifying strategies to mitigate the disruption services caused by COVID-19 pandemic.We conducted a cross-sectional study TB and HIV low middle-income countries (LMIC). Between May 12 August 6, 2020, we collected qualitative quantitative data using an online survey 11 languages. We used descriptive statistics thematic analysis analyse responses.669 respondents from 64 completed survey. Over 40% stated that it was either...
To determine if temporarily blocking sex steroids prior to stem cell transplantation can increase thymus function and thus enhance the rate of T regeneration.This was a pilot study luteinizing hormone-releasing hormone agonist (LHRH-A) goserelin given 3 weeks allogeneic or autologous hemopoietic administered up months posttransplantation. Patients (with without LHRH-A administration) were assessed from 1 week 12 posttransplantation for multiple immunologic variables by flow cytometry...
Background Mycobacterium tuberculosis (MTb) infects approximately 2 billion people world-wide resulting in almost million deaths per year. Determining biomarkers that distinguish different stages of (TB) infection and disease will provide tools for more effective diagnosis ultimately aid the development new vaccine candidates. The current diagnostic kits utilising production IFN-γ response to TB antigens can detect MTb but are unable between disease. aim this study was assess if use a longer...
Background. Anemia is common in tuberculosis, and multiple etiologies necessitate targeted interventions. The proportion of iron-responsive anemia due to iron deficiency compared with iron-unresponsive impaired absorption/redistribution from tuberculosis-associated immune activation or inflammation unknown. This impedes selection safe effective treatment appropriate intervention timing. Methods. Baseline hemoglobin, ferritin, hepcidin, soluble transferrin receptor (sTfR), were measured 45...
ABSTRACT The Mycobacterium tuberculosis genome includes the large family of pe_pgrs genes, whose functions are unknown. Because precedents in other pathogens which gene families showing high sequence variation involved antigenic variation, a similar role has been proposed for genes. However, impact immune selection on genes not examined. Here, we sequenced 27 94 clinical strains from five phylogenetic lineages M. complex (MTBC). We found that were overall more diverse than remainder MTBC...
IL-17 producing cells have been shown to be important in the early stages of Mycobacterium tuberculosis (Mtb) infection animal models. However, there is very little data on role human studies TB. We recruited TB patients and their highly exposed contacts who were further categorised based results from an IFN-γ release assay (IGRA): 1) IGRA+ at recruitment (latently infected (LTBI)), 2) IGRA negative 6 months (non-converters (NC)) 3) positive (converters (C)). Whole blood was stimulated with...
Background: Tuberculosis (TB) remains a major threat to global health. Currently, diagnosis of active TB is hampered by the lack specific biomarkers that discriminate disease from other (lung) diseases or latent infection (LTBI). Integrated human gene expression results have shown genes encoding complement components, in particular different C1q chains, were expressed at higher levels compared LTBI. Methods: protein determined using ELISA sera patients, geographically distinct populations,...
Up to fifty percent of microbiologically cured tuberculosis (TB) patients may be left with permanent, moderate or severe pulmonary function impairment. Very few studies have systematically examined outcomes in understand the pathophysiologic basis and long-term socio-economic consequences this injury. The planned multi-country, multi-centre observational TB cohort study, aims advance understanding clinical, microbiological, immunological risk factors affecting outcome TB. It will also...