A5021986665- Tuberculosis Research and Epidemiology
- T-cell and B-cell Immunology
- Mycobacterium research and diagnosis
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- Hepatitis B Virus Studies
- Pneumocystis jirovecii pneumonia detection and treatment
- HIV Research and Treatment
- Infectious Diseases and Tuberculosis
- Monoclonal and Polyclonal Antibodies Research
- Hepatitis C virus research
- Diagnosis and treatment of tuberculosis
- Leprosy Research and Treatment
- Immune Response and Inflammation
- COVID-19 Clinical Research Studies
- Acute Lymphoblastic Leukemia research
- Health Policy Implementation Science
- Pneumonia and Respiratory Infections
- Immunodeficiency and Autoimmune Disorders
- SARS-CoV-2 and COVID-19 Research
- Chronic Lymphocytic Leukemia Research
- Lymphoma Diagnosis and Treatment
- HIV/AIDS Research and Interventions
- Glycosylation and Glycoproteins Research
- Cervical Cancer and HPV Research
Armauer Hansen Research Institute
2016-2025
Haramaya University
2019-2020
All Africa Leprosy Tuberculosis and Rehabilitation Training Centre
2015-2020
Ananthapuri Hospitals and Research Institute
2017-2019
Karolinska Institutet
2015
Stellenbosch University
2013
Addis Ababa University
2013
Animal and Plant Health Agency
2013
Swiss Tropical and Public Health Institute
2013
University of Basel
2013
The Pgp-1 glycoprotein was identified on a minor (27%) subset of peripheral Lyt-2+ or L3T4+ T cells. In contrast, mature medullary-type thymocytes (Lyt-2+ L3T4-, Lyt-2- L3T4+) were nearly devoid cells expressing detectable surface Pgp-1. appearance Pgp-1- found to be thymus dependent, as demonstrated by the diminished proportion after thymectomy and their virtual absence in athymic nude mice. subsequent acquisition stable differentiation event occurring concomitantly with primary antigenic...
Rationale: Contacts of patients with tuberculosis (TB) constitute an important target population for preventive measures because they are at high risk infection Mycobacterium and progression to disease.Objectives: We investigated biosignatures predictive ability incident TB.Methods: In a case–control study nested within the Grand Challenges 6-74 longitudinal HIV-negative African cohort exposed household contacts, we employed RNA sequencing, PCR, pair ratio algorithm in training/test set...
A minor subset of immature (CD4-,8-) thymocytes that lack expression the B2A2 antigen was found to express low levels surface TCR protein as detected by mAbs F23.1 and KJ16 (reacting with products V beta 8 gene family). Interestingly, F23.1/KJ16 determinants were expressed on a two- three-fold higher proportion B2A2- than mature lymph node T cells in four independent haplotypes. When expanded short-term culture PMA calcium ionophore, retained their overexpression showed fivefold elevated...
New biomarkers of tuberculosis (TB) risk and disease are critical for the urgently needed control ongoing TB pandemic. In a prospective multisite study across Subsaharan Africa, we analyzed metabolic profiles in serum plasma from HIV-negative, TB-exposed individuals who either progressed to 3-24 months post-exposure (progressors) or remained healthy (controls). We generated trans-African biosignature TB, which identifies future progressors both on blinded test samples external data sets...
Abstract The World Health Organization [WHO] recommends a genotype-specific human papillomavirus [HPV] vaccination as primary prevention strategy to control the burden of cervical cancer globally. In Ethiopia, where non-vaccine-targeted HPV genotypes have not been adequately studied, initiative was launched in 2018 targeting HPV-6,-11, -16, and -18 for girls aged 14–18 years. co-existence both vaccine-targeted non-targeted is serious concern, it can accelerate progression. Therefore, this...
T lymphocytes with the surface phenotype CD4+8- and CD4-8+ are considered to be representative of functionally mature cells. We show here that adult murine thymus contains a subpopulation cells differ from found in periphery they do not express cell receptor-associated CD3 molecular complex. Such CD3-4-8+ thymocytes cortisone sensitive rapidly cycling situ. Furthermore, contrast cells, most low levels CD5 high B2A2 antigen. fail respond variety mitogenic stimuli vitro but give rise upon...
BackgroundTuberculosis (TB) remains a global health threat with 9 million new cases and 1.4 deaths per year. In order to develop protective vaccine, we need define the antigens expressed by Mycobacterium tuberculosis (Mtb), which are relevant immunity in high-endemic areas. MethodsWe analysed responses 23 Mtb total of 1247 subjects different HIV TB status across 5 geographically diverse sites Africa (South Africa, The Gambia, Ethiopia, Malawi Uganda). We used 7-day whole blood assay followed...
We investigated host-derived biomarkers that were previously identified in QuantiFERON supernatants, a large pan-African study. recruited individuals presenting with symptoms of pulmonary TB at seven peripheral healthcare facilities six African countries, prior to assessment for disease. then evaluated the concentrations 12 stored supernatants using Luminex platform. Based on laboratory, clinical and radiological findings pre-established algorithm, participants classified as disease or other...
Abstract Background COVID-19 pandemic has a devastating impact on the economies and health care system of sub-Saharan Africa. Healthcare workers (HWs), main actors system, are at higher risk because their occupation. Serology-based estimates SARS-CoV-2 infection among HWs represent measure HWs’ exposure to virus could be used as guide prevalence in community valuable combating COVID-19. This information is currently lacking Ethiopia other African countries. study aimed develop an in-house...
Cervical cancer is a vaccine-preventable sexually transmitted disease. In the year 2020, there were an estimated 604,000 new cases and 342,000 deaths worldwide. Although its incidence global, it much higher in sub-Saharan African countries. Ethiopia, scarcity of data about prevalence high-risk HPV infection association with cytological profiles. Therefore, this study was conducted to fill information gap. A hospital-based cross-sectional from April 26 August 28, 2021, enrolled 901 active...
The impact of diabetes mellitus on tuberculosis (TB) treatment outcomes has not been well investigated in most sub-Saharan countries including Ethiopia. current study aimed to determine the association between and unsuccessful TB among drug-susceptible patients treated at selected health facilities Addis Ababa,
Abstract Intestinal dendritic cells (DCs) play key roles in mediating tolerance to commensal flora and inflammatory responses against mucosal pathogens. The mechanisms by which intestinal “conditioning” influences human DC microbial stimuli remain poorly understood. Infections with viruses, such as HIV-1, that target tissue result epithelial barrier breakdown increased translocation of bacteria into the lamina propria (LP). It is unclear whether innate LP concurrent viral bacterial influence...
Dengue is one of the most common arboviral diseases with increased outbreaks annually in tropical and subtropical areas. In Ethiopia, there are no data regarding clinical, hematological biochemical parameters which very important clinical management dengue patients. Hence this study was carried out to provide first baseline profiles patients infected virus. A cross-sectional among febrile northwest Ethiopia from March 2016 May 2017. Blood samples were collected presumed cases tested against...
The immunologic environment during HIV/M. tuberculosis co-infection is characterized by cytokine and chemokine irregularities that have been shown to increase immune activation, viral replication, T cell dysfunction. We analysed ex vivo plasma samples from 17 HIV negative 16 pulmonary co infected cases using Luminex assay see impact of on level cytokines chemokines patients before after anti Tuberculosis treatment. median IFN-γ, IL-4, MCP-3, MIP-1β IP-10 was significantly different (P <...
Background Dengue is one of the most serious and rapidly spreading arboviral diseases in world. Despite many acute febrile illnesses Ethiopia, burden illness due to dengue country largely unknown. Thus, present study aimed provide first baseline data on seroprevalence associated risk factors virus (DENV) infection country. Methods A cross-sectional patients who were visiting Metema Humera hospitals Northwest Ethiopia from March 2016 May 2017 was conducted. Blood samples collected each...
The development of a non-sputum-based, point-of-care diagnostic test for tuberculosis (TB) is priority in the global effort to combat this disease, particularly resource-constrained settings. Previous studies have identified host biomarker signatures which showed potential, but there need validate and refine these as test. We recruited 1,403 adults presenting with symptoms suggestive pulmonary TB at primary healthcare clinics six countries from West, East Southern Africa. Of study cohort,...
Background: Flow cytometric characterisation of acute leukaemia is a key diagnostic approach for clinical management patients, but minimally practised in resource-constrained settings like Ethiopia.Objective: This study aimed to determine the immunophenotypes by flow cytometry at Tikur Anbessa Specialised Hospital, Addis Ababa, Ethiopia.Methods: A cross-sectional was conducted on adolescent and adult inpatients consecutively admitted from April 2019 June 2021. Peripheral blood samples were...
OBJECTIVE Thiopurine S-methyltransferase (TPMT) is a cytosolic transmethylase enzyme that catalyzes the S-methylation of thiopurine drugs, mainstay acute lymphoblastic leukemia treatment. TPMT activity shows interindividual variability, which can partly be explained by genetic variants in gene. In this study, we aimed to investigate concordance between genotype and phenotype gene cohort patients with from Ethiopia. METHODS present 73 on treatment 32 completed were included. was measured...