A5056006726- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- HIV Research and Treatment
- T-cell and B-cell Immunology
- HIV-related health complications and treatments
- HIV/AIDS Research and Interventions
- Gut microbiota and health
- vaccines and immunoinformatics approaches
- Cancer Immunotherapy and Biomarkers
- IL-33, ST2, and ILC Pathways
- CAR-T cell therapy research
- HIV/AIDS drug development and treatment
- RNA Interference and Gene Delivery
- Cytomegalovirus and herpesvirus research
- Immune Response and Inflammation
- Eosinophilic Esophagitis
- Spondyloarthritis Studies and Treatments
- Rheumatoid Arthritis Research and Therapies
- Diet and metabolism studies
- Reproductive tract infections research
- Psoriasis: Treatment and Pathogenesis
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- COVID-19 Clinical Research Studies
- Syphilis Diagnosis and Treatment
University of Colorado Denver
2013-2024
University of Colorado Anschutz Medical Campus
2011-2023
The Medical Center of Aurora
2020
Office of Infectious Diseases
2013-2020
University of Colorado Hospital
2014-2017
National Jewish Health
2013
University of Colorado Health
2001-2007
Denver Health Medical Center
2007
Infectious Disease Consultants
2005
University of Colorado System
2003
Background. Defining the association of non–AIDS-defining events with inflammation and immune activation among human immunodeficiency virus (HIV)–infected persons antiretroviral therapy (ART)–associated virological suppression is critical to identifying interventions decrease occurrence these events. Methods. We conducted a case-control study HIV-infected subjects who had achieved within 1 year after ART initiation. Cases were patients experienced events, defined as myocardial infarction,...
The impacts of interferon (IFN) signaling on COVID-19 pathology are multiple, with both protective and harmful effects being documented. We report here a multiomics investigation systemic IFN in hospitalized patients, defining the biosignatures associated varying levels 12 different type I, II, III IFNs. antiviral transcriptional response circulating immune cells is strongly specific subset IFNs, most prominently IFNA2 IFNG. In contrast, proteomics signatures indicative endothelial damage...
Background. The relationships of inflammation, immune activation, and immunosenescence markers with functional impairment in aging human immunodeficiency virus type 1 (HIV-1)–infected persons are unknown.
To explore darunavir/ritonavir (DRV/r) plus raltegravir (RAL) combination therapy in antiretroviral-naive patients.
Altered tryptophan catabolism has been identified in inflammatory diseases like rheumatoid arthritis (RA) and spondyloarthritis (SpA), but the causal mechanisms linking metabolites to disease are unknown. Using collagen-induced (CIA) model, we alterations metabolism, specifically indole, that correlated with disease. We demonstrated both bacteria dietary were required for indole supplementation was sufficient induce their absence. When mice CIA on a low-tryptophan diet supplemented observed...
Abstract Elevated expression of inhibitory receptors on virus-specific T cells has been implicated as a mechanism by which viruses evade host immune surveillance. Blockade these pathways during chronic infection leads to increased cell function and improved control viral replication. To explore the association between costimulatory HIV replication, we examined programmed death 1 (PD-1), CTLA-4, Ig domain mucin 3 (TIM-3), CD28 HIV-specific CD4+ from HIV-infected subjects. Greater than 30%...
People with HIV (PWH) experience accentuated biological aging, as defined by markers of inflammation, immune dysfunction, and the epigenetic clock. They also have an elevated risk multiple age-associated comorbidities. To discuss current knowledge, research gaps, priorities in aging age-related comorbidities treated infection, NIH program staff organized a workshop held Bethesda, Maryland September 2019. This review article describes highlights discussions led Pathogenesis/Basic Science...
Innate lymphoid cells (ILCs) are a diverse family of that play critical roles in mucosal immunity. One subset the ILC family, Group 3 ILCs (ILC3s), has been shown to aid gut homeostasis through production IL-22. IL-22 promotes its functional effect on epithelial barrier. When barrier integrity is compromised, such as Human Immunodeficiency Virus (HIV) infection and inflammatory bowel disease (IBD), microbes from lumen translocate into lamina propria, inducing multitude potentially pathogenic...
Abstract DNA-based immunization strategies designed to elicit cellular antitumor immunity offer an attractive alternative protein- or peptide-based approaches. In the present study we have evaluated feasibility of DNA vaccination for induction CTL reactivity five different melanoma Ags in vitro. Cultured, monocyte-derived dendritic cells (DC) were transiently transfected with plasmid encoding human MART-1/Melan-A, pMel-17/gp100, tyrosinase, MAGE-1, MAGE-3 by particle bombardment and used...
Abstract Intestinal dendritic cells (DCs) play key roles in mediating tolerance to commensal flora and inflammatory responses against mucosal pathogens. The mechanisms by which intestinal “conditioning” influences human DC microbial stimuli remain poorly understood. Infections with viruses, such as HIV-1, that target tissue result epithelial barrier breakdown increased translocation of bacteria into the lamina propria (LP). It is unclear whether innate LP concurrent viral bacterial influence...
Physiologic aging has been associated with gut dysbiosis. Although short exercise interventions have linked to beneficial changes in microbiota younger adults, limited data are available from older populations. We hypothesized that would produce shifts and short-chain fatty acid (SCFA) levels persons.Stool samples were collected before at completion of a supervised 24-week cardiovascular resistance intervention among 50-75-year-old participants. SCFA analyzed by gas chromatography microbiome...
Human peripheral blood contains antigen-presenting cells (APC), including dendritic (DC) and monocytes, that may encounter microbes have translocated from the intestine to periphery in disease states like HIV-1 infection inflammatory bowel disease. We investigated response of DC monocytes mononuclear (PBMC) a panel representative commensal enteric bacteria, Escherichia coli, Enterococcus sp., Bacteroides fragilis. All three bacteria induced significant upregulation maturation activation...
BackgroundHIV-1 infection and physiological aging are independently linked to elevated systemic inflammation changes in enteric microbial communities (dysbiosis). However, knowledge of the direct effect HIV on microbiome potential links is lacking.MethodsIn a cross-sectional study older people living with (PLWH) (median age 61.5 years, N = 14) uninfected controls 58 n 22) we compared stool microbiota, levels metabolites (short-chain fatty acid levels, SCFA) inflammatory biomarkers by...
We examined changes in soluble inflammatory cytokines and T-cell activation after antiretroviral therapy (ART) initiation an AIDS Clinical Trials Group (ACTG) nested case-control study. Cases were 143 human immunodeficiency virus (HIV)–infected adults who developed a non-AIDS event; 315 controls remained event-free. Specimens tested pre-ART, year 1 post-ART, at the visit preceding event. Conditional logistic regression evaluated associations of biomarker with events. Inflammatory most...
Elevated immune activation is associated with an increased risk of HIV acquisition. Tenofovir (TFV) has immunomodulatory properties in vitro, but how this extends vivo remains unknown.HIV-negative adults received daily coformulated TFV disoproxil fumarate 300 mg/emtricitabine (FTC) 200 mg for 30 days followed by a 30-day washout. Markers T-cell activation, inflammation, and cytokines were measured before drug on (on drug) 60 (30-day washout). Data analyzed using one-way analysis...
An important aspect of vaccine development involves delivery antigens to antigen-presenting cells for the induction potent antigen-specific T lymphocyte responses. We investigated effect a cationic liposome, lipofectin, on whole proteins human dendritic (DCs) derived from blood mononuclear by culture in interleukin 4 and granulocyte-monocyte colony-stimulating factor stimulation immunodeficiency virus type 1 (HIV-1)-specific memory cytotoxic (CTL) Delivery HIV-1 Gag, Pol, Env DCs lipofectin...